A rapid procedure for the purification of ferredoxin from clostridia using polyethyleneimine

Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea

“…Ferredoxin and hydrogenase from C. pasteurianum were prepared according Schönheit et al (23) and Li et al (19), respectively.…”

A Bacterial Electron-bifurcating Hydrogenase

“…Physiological hydrogenase activity with NAD ϩ and ferredoxin was measured in buffer 1 at 340 nm and 430 nm, respectively (⑀ NADH ϭ 6.2 mM Ϫ1 cm Ϫ1 , ⑀ Fd ϭ 13.1 mM Ϫ1 cm Ϫ1 ). Ferredoxin was purified from Clostridium pasteurianum as described (28). For inhibition studies with CO and determination of the temperature optimum, the enzyme was preincubated for 5 min with CO at 30°C or at the temperature indicated, respectively.…”

The Ferredoxin:NAD+ Oxidoreductase (Rnf) from the Acetogen Acetobacterium woodii Requires Na+ and Is Reversibly Coupled to the Membrane Potential

“…Measurement of CO-mediated ferredoxin:NAD ϩ oxidoreductase activity was performed at a pressure of 0.5 ϫ 10 5 Pa of CO with 30 g/ml Acs/CODH, 30 M ferredoxin, and 150 g/ml washed membranes. Ferredoxin was purified from Clostridium pasteurianum as described (13). The reaction was started by the addition of NAD ϩ .…”