A rapid procedure for bacterial identification and antimicrobial susceptibility testing directly from positive blood cultures

Abstract

This semi-automatic procedure only takes 3.5 h to obtain bacterial identification and antimicrobial susceptibility testing results starting from positive blood cultures.

Rapid determination of Proteus mirabilis susceptibility to antibiotics using infrared spectroscopy in tandem with random forest

“…As expected, the spectral differences between the resistant and sensitive isolates of P. mirabilis are minute (Figures 2-4) and subtle. These differences appear mainly in spectral features' intensities because they reflect the minor biochemical changes in resistivity [54]. These minor changes are supported by findings in our previous studies regarding E. coli and K. pneumoniae [28,29,53].…”

“…Other studies reported the application of surfaceenhanced Raman scattering for a rapid bacterial antibiotic susceptibility test and minimum inhibitory concentration of S. aureus and wild-type E. coli, as well as clinical isolates [50][51][52][53][54][55]. Combined Raman Spectroscopy and dielectrophoresis were used to determine the susceptibility of E. coli MG1655 to the second-generation fluoroquinolone antibiotic [56][57][58][59].…”

Recent advances of Raman spectroscopy for the analysis of bacteria

“…93 Samples were incubated in D 2 O-containing antibiotics and then C-D Raman bands were measured to distinguish bacterial strains. Similar studies were conducted for blood cultures 94 and clinical strains. 95,96 Similar to antibiotics, there are studies that investigated bacterial responses to environmental stressors, 97,98 heavy metals, and herbicides.…”

“… 93 Samples were incubated in D 2 O‐containing antibiotics and then C‐D Raman bands were measured to distinguish bacterial strains. Similar studies were conducted for blood cultures 94 and clinical strains. 95 , 96 …”

Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging

“…CRS imaging methods provide similar capabilities but at a much higher throughput. SRS imaging has shown to be a powerful tool for rapid antimicrobial susceptibility testing. − For example, Chen et al developed a metabolic imaging method to determine antifungal susceptibility within 4 h . By monitoring the intracellular conversion of D 2 O or glucose- d 7 to C–D bonds in proteins, lipids, and DNA, the authors successfully demonstrated the use of metabolic change as a marker for rapid susceptibility testing (Figure a.i).…”