A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Toribio, Luz; Handali, Sukwan; Marin, Yazmin; Pérez, Erika; Castillo, Yesenia; Bustos, Javier A.; O’Neal, Seth E.; Garcı́a, Héctor H.

doi:10.4269/ajtmh.22-0598

Cited by 5 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most promising pair of capture and detection mAbs (TsW8 and TsW5, respectively) was used in a sandwich ELISA format, as described here. A recent study has also proved an optimal performance of this assay in detecting urinary antigen in a rapid test format [36]. TsW8 and TsW5 recognized surface structures of the neck and cyst wall, and they have already demonstrated specificity against E. granulosus VF, T. hydatigena VF, and F. hepatica E/S [26].…”

Section: Discussionmentioning

confidence: 92%

Consistent Measurement of Parasite-Specific Antigen Levels in Sera of Patients with Neurocysticercosis Using Two Different Monoclonal Antibody (mAb)-Based Enzyme-Linked Immunosorbent Assays

et al. 2023

Self Cite

View full text Add to dashboard Cite

Monoclonal antibody (mAb)-based enzyme-linked immunosorbent assay (ELISA) is a complementary diagnosis technique for neurocysticercosis (NCC), which detects circulating parasite antigen (Ag) indicative of viable infection and Ag levels that correlate well with the parasite burden. In this study, we compared the performance of two Ag-ELISA techniques for the detection of NCC. We assessed the agreement between our in-house TsW8/TsW5 Ag-ELISA and the widely used B158/B60 Ag-ELISA for measuring T. solium antigen levels in the sera from 113 patients with calcified, parenchymal, and subarachnoid NCC. Concordance was demonstrated evaluating the limits of agreement (LoAs) stratified by the type of NCC. Both ELISA’s detected 47/48 (97.8%) subarachnoid NCC cases. In parenchymal and calcified NCC, the B158/B60 Ag-ELISA detected 19/24 (79.2%) and 18/41 (43.9%) cases, while the TsW8/TsW5 Ag-ELISA detected 21/24 (87.5%) and 13/41 (31.7%), respectively. Parenchymal and calcified NCC obtained a perfect agreement (100%), indicating that all sample results were within the predicted LoA, while for subarachnoid NCC, the agreement was 89.6%. The high concordance between the assays was confirmed by Lin’s concordance coefficient (LCC = 0.97). Patients with viable parenchymal NCC (LCC = 0.95) obtained the highest concordance between assays, followed by subarachnoid NCC (LCC = 0.93) and calcified NCC (LCC = 0.92). The TsW8/TsW5 Ag-ELISA and B158/B60 Ag-ELISA showed high Ag measurement correlations across diverse types of NCC.

show abstract

Section: Discussionmentioning

confidence: 92%

Consistent Measurement of Parasite-Specific Antigen Levels in Sera of Patients with Neurocysticercosis Using Two Different Monoclonal Antibody (mAb)-Based Enzyme-Linked Immunosorbent Assays

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…[ 23 ] did not report that the mAbs TsW8/TsW5 cross-react with antigens from the vesicular fluid of T. hydatigena cysts [ 23 ], this does not necessarily exclude the possibility of reaction against other antigenic components of the parasite as demonstrated in our study. However, cross-reaction with T. hydatigena is not a limitation for its potential use as a diagnostic tool for antigen detection in human NCC [ 32 – 34 ], given that this parasite only infects humans very exceptionally. On the other hand, the T. hydatigena cross-reaction of the TsW8/TsW5 Ag-ELISA in experimentally infected pigs limits its potential use to estimate T. solium infection in porcine populations in areas co-endemic with T. hydatigena [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Concordance between two monoclonal antibody-based antigen detection enzyme-linked immunosorbent assays for measuring cysticercal antigen levels in sera from pigs experimentally infected with Taenia solium and Taenia hydatigena

Arroyo,

Toribio,

Garrido

et al. 2024

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

Background Antigen detection in Taenia solium cysticercosis confirms viable infection in the intermediate host (either pig or human). The reference B158/B60 monoclonal antibody (mAb)-based Ag-enzyme-linked immunosorbent assay (ELISA) has acceptable levels of sensitivity and specificity in human neurocysticercosis with multiple brain cysts, although its sensitivity is lower in cases with single brain cysts, whereas in porcine cysticercosis the assay specificity is affected by its frequent cross-reaction with Taenia hydatigena, another common cestode found in pigs. Our group has produced 21 anti-T. solium mAbs reacting against antigens of the whole cyst, vesicular fluid, and secretory/excretory products, identifying TsW8/TsW5 as the most promising pair of mAbs for an Ag-ELISA. Methods We report the use of the TsW8/TsW5 Ag-ELISA to measure cysticercus antigen levels [expressed as optical density (OD) values] in two panels of sera collected from day 0 (baseline) to day 90 postinfection (PI) from pigs experimentally infected with T. solium (n = 26) and T. hydatigena (n = 12). At baseline and on days 28 and 90 PI, we used Bland–Altman (BA) analysis and Lin’s concordance correlation coefficients (CCC) to determine the concordance between the TsW8/TsW5 and the B158/B60 Ag-ELISA. Results The TsW8/TsW5 Ag-ELISA was able to efficiently measure circulating antigen levels in T. solium-infected pigs, similar to that obtained with the B158/B60 Ag-ELISA. Almost all paired log-OD differences between assays were within the limits of agreement (LoA) in the BA analysis at baseline and on days 28 and 90 PI (92.3%, 100%, and 100%, respectively), and a high concordance of log-ODs between assays was also found (Lin’s CCC: 0.69, 0.92, and 0.96, respectively, all P < 0.001). In pigs infected with T. hydatigena, almost all paired log-OD differences were within the LoA in the BA analysis, whereas the concordance of log-ODs between assays was low at baseline (Lin’s CCC: 0.24) but increased on days 28 and 90 PI (Lins’ CCC: 0.88 and 0.98, P < 0.001). Conclusions/significance The TsW8/TsW5 Ag-ELISA recognizes antigens in pigs with T. solium cysticercosis and is highly concordant with the B158/B60 Ag-ELISA. However, its diagnostic use is hampered by cross-reactions with T. hydatigena, as in other mAb-based Ag-ELISAs. Graphical Abstract

show abstract

“…Although the previous study of Paredes did not report that mAbs TsW8 / TsW5 cross-react with antigens from vesicular uid of T. hydatigena [23], this does not necessarily exclude the possibility of reaction against other antigenic components of the parasite as demonstrated in our study. However, cross reaction with T. hydatigena is not a limitation for its potential use as a diagnostic tool for antigen detection in human NCC [31,33],…”

Section: Discussionmentioning

confidence: 99%

Concordance between two monoclonal antibody (mAb)-based enzyme-linked immunosorbent assays for the measurement of cysticercal antigen levels in sera of pigs experimentally infected with Taenia solium and Taenia hydatigena

Arroyo,

Toribio,

Garrido

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background Antigen detection in Taenia solium cysticercosis confirms viable infection in the intermediate host (either pig or human). The reference B158 / B60 antigen-detection ELISA (Ag-ELISA) has high levels of sensitivity and specificity in human neurocysticercosis (94% and 99% according to the manufacturer), although its sensitivity is lower in cases with single brain cysts, whereas in porcine cysticercosis the assay performance is affected by its frequent cross-reaction with Taenia hydatigena. Our group has produced 21 anti-T. solium monoclonal antibodies (mAbs) reacting against antigens of the whole cyst, vesicular fluid, and secretory/excretory products, identifying TsW8 / TsW5 as the most promising pair of mAbs for an Ag-ELISA. Methods We report the use of our TsW8 / TsW5 Ag-ELISA for the measurement of parasite antigen levels in two panels of sera from pigs experimentally infected with T. solium (n = 26) and T. hydatigena (n = 12) from day 0 to day 90 post-infection (PI) and assessed the concordance of our assay with the reference B158 / B60 Ag-ELISA using Bland-Altman (BA) plots and Lin’s concordance coefficients (LCC). Results Our TsW8 / TsW5 Ag-ELISA measured antigen levels efficiently during the course of T. solium cysticercosis infections in pigs with a strong agreement with the reference B158 / B60 Ag-ELISA in almost all paired log-transformed optical density (OD) values. The limits of agreement (LoA) in the BA plot at days 0, 28, and 90 PI were 92.3%, 100% and 100%, respectively, and high concordance between assays was also found (LCC: 0.69, 0.92, and 0.96, respectively, all P < 0.001). In pigs infected with T. hydatigena, almost all paired log-transformed ODs were also within the LoA, whereas concordance between assays was low or moderate at days 0 and 28 PI, (LCC: 0.24 and 0.88), but higher at day 90 PI (LCC: 0.98, P < 0.001). Conclusions / significance: The TsW8 / TsW5 Ag-ELISA recognizes antigens in pigs with T. solium cysticercosis and is highly concordant with the B158 / B60 Ag-ELISA. However, its diagnostic use is hampered by cross-reactions with T. hydatigena, as in other mAb antigen detection assays.

show abstract

A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Cited by 5 publications

References 12 publications

Consistent Measurement of Parasite-Specific Antigen Levels in Sera of Patients with Neurocysticercosis Using Two Different Monoclonal Antibody (mAb)-Based Enzyme-Linked Immunosorbent Assays

Consistent Measurement of Parasite-Specific Antigen Levels in Sera of Patients with Neurocysticercosis Using Two Different Monoclonal Antibody (mAb)-Based Enzyme-Linked Immunosorbent Assays

Concordance between two monoclonal antibody-based antigen detection enzyme-linked immunosorbent assays for measuring cysticercal antigen levels in sera from pigs experimentally infected with Taenia solium and Taenia hydatigena

Concordance between two monoclonal antibody (mAb)-based enzyme-linked immunosorbent assays for the measurement of cysticercal antigen levels in sera of pigs experimentally infected with Taenia solium and Taenia hydatigena

Contact Info

Product

Resources

About