A rapid PCR protocol for identification of differentially expressed genes from a cDNA library.

The Polymerase Chain Reaction in Molecular and Micro-biology

“…The use of the PCR for cloning, mutation, gene fusion and sequencing as described above, although highly significant, only scratches the surface of the many exploitations of this powerful technique to simplify or speed up molecular biological methods including: Si protection assays (Blakely and Carman, 1991), DNaseI footprinting (Tanguay, Pfeifer and Riggs, 1990), DNA-protein binding studies (Higuchi, Krummel and Saiki, 1988;Kinzler and Vogelstein, 1989;Pollock and Treisman, 1990;Rudert and Truce , 1990), genomic walking (Fors, Saavedra and Hood, 1990;Rosenthal and Jones, 1990;Arnold and Hodgson, 1991;Parks, Chang and Shenk, 1991) and assaying gene expression (Mutchler, Klemish and Russo, 1992;Sperisen et al, 1992). The potential of the PCR in research applications appears to be limited only by the imagination of research scientists, and novel methods are under continuous evolution.…”