A rapid multiplex assay of human malaria parasites by digital PCR

Liu, Dong; Li, Weijia; Xu, Qing; Gu, Jianfei; Kang, Zhihua; Chen, Jian; Xu, Xin; Zhang, Xinju; Zhang, Xiuming; Jiang, Hanqiang; Guan, Ming

doi:10.1016/j.cca.2022.12.001

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…The detection of possible problems and the tuning of thermal cycle parameters for improved performance are made easier with this information. The efficiency of reactant mixing is improved by looping in the design of microfluidic PCR chips, which boosts amplification performance [7,37]. Looping enables the controlled transport and dispersion of target DNA, primers, and enzymes by providing circular flow channels within the chip.…”

Section: Resultsmentioning

confidence: 99%

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Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Austria,

Garcia,

Caparanga

et al. 2023

Computation

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Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for the detection of malaria. However, the collection and transportation of samples and the processing and dissemination of results via conventional PCR, especially when used for routine clinical practice, can hamper the technique’s sensitivity and specificity. The rampancy of such disease in the Philippines is aggravated by the limited supply of medical machinery and the poor economic state of the country; thus, the need to innovate a device for the early detection of malaria is necessary. With that, this study focuses on designing a microfluidic device that will mimic the function of a conventional genus-specific PCR based on the 18S rRNA gene to detect malaria parasites (Plasmodium falciparum) at low-grade parasitemia. The design was intended to be portable, accessible, and economical, which none from past literature has dealt with specifically for malaria detection. This in silico design is a first in the country specially crafted for such reasons. The proposed device was developed and simulated using ANSYS software for Computational Fluid Dynamics (CFD) analyses. The simulation shows that adding loops to the design increases its relative deviation but minimally compared to having only a straight path design. This indicates that looping is acceptable in designing a microfluidic device to minimize chip length. It was also found that increasing the cross-sectional area of the fluid path decreases the efficiency of the design. Lastly, among the three materials utilized, the chip made of polypropylene is the most efficient, with a relative deviation of 0.94 compared to polycarbonate and polydimethylsiloxane, which have relative deviations of 2.78 and 1.92, respectively. Future researchers may mesh the 44-cycle microfluidic chip due to the limitations of the software used in this study, and other materials, such as biocomposites, may be assessed to broaden the application of the design.

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Section: Resultsmentioning

confidence: 99%

“…The foundation of PCR is the enzymatic amplification of specific DNA sequences. It uses the capacity to synthesize DNA strands of the heat-resistant Taq DNA polymerase [7]. The specific DNA region of the malaria parasite is amplified exponentially, producing millions of copies that are readily detectable.…”

Section: Introductionmentioning

confidence: 99%

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Austria,

Garcia,

Caparanga

et al. 2023

Computation

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“…Applications of dPCR have been developed for the diagnosis of protozoa that have an important epidemiological impact in specific geographical areas, such as Trypanosoma cruzi [49] and Plasmodium spp. [50][51][52] with good results. It should be taken into account that a reliable count of the parasitisation index, in the case of Plasmodium spp., is important to define the appropriate treatment so dPCR can make a contribution in this respect.…”

Section: Applications In Parasitology and Protozoamentioning

confidence: 94%

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Sancha Dominguez,

Cotos Suárez,

Sánchez Ledesma

et al. 2024

Diagnostics

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Infectious diseases account for about 3 million deaths per year. The advent of molecular techniques has led to an enormous improvement in their diagnosis, both in terms of sensitivity and specificity and in terms of the speed with which a clinically useful result can be obtained. Digital PCR, or 3rd generation PCR, is based on a series of technical modifications that result in more sensitive techniques, more resistant to the action of inhibitors and capable of direct quantification without the need for standard curves. This review presents the main applications that have been developed for the diagnosis of viral, bacterial, and parasitic infections and the potential prospects for the clinical use of this technology.

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Section: Applications In Parasitology and Protozoamentioning

confidence: 94%

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Sancha Domínguez,

Cotos Suárez,

Sánchez Ledesma

et al. 2024

Preprint

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A Infectious diseases account for about 3 million deaths per year. The advent of molecular techniques has led to an enormous improvement in their diagnosis, both in terms of sensitivity and specificity and in terms of the speed with which a clinically useful result can be obtained. Digital PCR, or 3rd generation PCR, is based on a series of technical modifications that result in more sensitive techniques, more resistant to the action of inhibitors and capable of direct quantification, without the need for standard curves.This review presents the main applications that have been developed for the diagnosis of viral, bacterial and parasitic infections, and the potential prospects for the clinical use of this technology.

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A rapid multiplex assay of human malaria parasites by digital PCR

Cited by 6 publications

References 23 publications

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

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