A rapid method to estimate Westergren sedimentation rates

Alexy, Tamás; Pais, Eszter; Meiselman, Herbert J.

doi:10.1063/1.3212564

Cited by 12 publications

(6 citation statements)

References 10 publications

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“…In most evaluations, new analyser methods were compared with the standardized method with diluted samples (Thomas & Karpic, 1993; Happe et al. , 2002; Romero, Munoz & Ramirez, 2003; Al‐Fadhli & Al‐Awadhi, 2005; Ajubi, Bakker & van den Berg, 2006; Mahlangu & Davids, 2008; Shelat, Chacosky & Shibutani, 2008; Alexy, Pais & Meiselman, 2009; Hardeman et al. , 2010a,b; Perovic, Bakovic & Valcic, 2010).…”

Section: Review Of the Literature: Use Of The Esr Reference And Standmentioning

confidence: 99%

“…, 2002; Al‐Fadhli & Al‐Awadhi, 2005; Ozdem et al. , 2006; Osei‐Bimpong, Meek & Lewis, 2007; Mahlangu & Davids, 2008; Shelat, Chacosky & Shibutani, 2008; Alexy, Pais & Meiselman, 2009; Perovic, Bakovic & Valcic, 2010). Some of these involve automation of the Westergren method with diluted or undiluted samples while others use very new technologies.…”

Section: Evaluation Of New Analysersmentioning

confidence: 99%

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ICSH review of the measurement of the erythocyte sedimentation rate

Jou¹,

Lewis²,

Briggs³

et al. 2011

International Journal of Laboratory Hematology

116

130

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In recognition of the need for a standardization of the measurement of the erythrocyte sedimentation rate (ESR), the International Council for Standardization in Haematology makes the following recommendations: (i) The reference method for measurement of the ESR should be based on the Westergren method, which is a specific test for the ESR, with modifications, (ii) The reference method for measurement of the ESR should use either whole blood anticoagulated with EDTA and later diluted with sodium citrate or saline (4 : 1) or whole blood anticoagulated with sodium citrate (4 : 1) in Westergren pipettes, (iii) The ESR pipettes can be of glass or plastic (with specific characteristics). It must be colourless; a minimum sedimentation scale of 200 mm, a minimum bore of 2.55 mm, which should be constant within 5%. A protocol for the evaluation of alternative methodologies against the reference method is outlined: The new technologies must be tested over a range of ESR values of 2-120 mm. In this comparison, 95% of the differences should be 5 mm or less, with larger differences associated with higher ESR values. A minimum of 40 samples should be tested in 3 different groups of values: 1-20, 21-60 and more than 60 mm. The statistical methods recommended for ESR evaluations are the coefficient of correlation, the Passing-Bablock regression and the Bland-Altman statistical method. This reference method replaces all earlier standardized and reference methods.

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Section: Review Of the Literature: Use Of The Esr Reference And Standmentioning

confidence: 99%

Section: Evaluation Of New Analysersmentioning

confidence: 99%

ICSH review of the measurement of the erythocyte sedimentation rate

Jou¹,

Lewis²,

Briggs³

et al. 2011

International Journal of Laboratory Hematology

116

130

View full text Add to dashboard Cite

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“…Among the blood components, RBCs and plasma significantly determine the biophysical properties of blood, such as viscosity, 2-6 viscoelasticity, [7][8][9] the hematocrit, [10][11][12][13][14][15] deformability, [16][17][18][19] RBC aggregation, [20][21][22][23][24] and the erythrocyte sedimentation rate (ESR). [25][26][27][28][29][30][31][32] Hemodynamic flows in the microcirculation, including arterioles, capillaries, and postcapillary venules, are predominantly determined by the biophysical properties of blood. 1 The biophysical properties of blood collected from the patients with hematological diseases and circulatory disorders are substantially altered.…”

Section: Introductionmentioning

confidence: 99%

Continuous and simultaneous measurement of the biophysical properties of blood in a microfluidic environment

Kang

2016

Analyst

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The biophysical properties of blood have been considered as promising indices for effectively screening the cardiovascular diseases. In this study, a method for the continuous and simultaneous measurement of the biophysical properties of blood, including viscosity, viscoelasticity, and RBC (red blood cell) aggregation is suggested, using a microfluidic device. The microfluidic device has two inlets (A, B), two outlets (A, B), two identical side channels, and one bridge channel. To sequentially induce steady and transient flows of blood samples, a blood sample is carefully delivered into the inlet (A) at a pulsatile flow rate (Q) (Q = 1 mL h, Q = 0 mL h, T = 240 s). By operating a pinch valve connected to the outlet (A), the blood flow is stopped or passed in the left-lower side channel. Three biophysical properties of the blood sample are quantified by analyzing the flow rate in the left-upper side channel (Q), the image intensity in the left-lower side channel (〈I〉), and the blood-filled width in the right-lower side channel (α). First, based on the modified parallel flow method, the blood viscosity (μ) is measured by analyzing the variation in α. Second, using a discrete fluidic circuit model, the time constant (λ) is evaluated by analyzing temporal variations in Q and 1/(1 - α). Then, the blood elasticity (G) is calculated by assuming the linear Maxwell model (i.e., λ = μ/G). Third, the RBC aggregation is quantified in terms of three parameters (〈I〉, A, and A) obtained by analyzing temporal variations in the image intensity. From the experimental demonstrations using various blood samples, it is concluded that the proposed method has the ability to measure the biophysical properties of blood with consistency, as compared with the previous methods. In the near future, the proposed method will be employed for evaluating variations in the biophysical properties of blood, circulating in the extracorporeal rat bypass loop.

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“…ESR is a non-specific, affordable, and reasonably easy haematological test for identifying inflammation that may be brought on by an infection, a cancer, or an autoimmune illness. (8) The ICSH has proposed alternative methods which are considered to be as reliable as conventional methods to evaluate ESR In order to validate the results (8,9). The statistical methods of bland and Altman and coefficient of correlation are frequently used to assess ESR.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Automated (ISED) ESR Estimation with Conventional (WESTERGREN) Method for Validation of Automated Results

Bashir¹,

Tashfeen²,

Akhtar³

et al. 2022

PJMHS

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Background: ESR inspite of being considered as a non-specific heamatological test is widely used for the detection of autoimmune diseases, infections and cancers. Although conventional gold standard methods are considered accurate and authentic but are having few biohazard risks and are more time consuming so are thought to be replaced by automated methods which are less time consuming and not much expensive but need to be validated by comparing them with conventional manual (Westergren) method. Objective: To compare the ESR values by conventional ESR method i.e. Westergren method with automated ESR estimation by ISED (ALCOR) to validate the results obtained by automated method. Study design: A Prospective cross-sectional study Study duration and settings: Study was conducted at Pathology Department Pak Emirates Military Hospital Rawalpindi from November 2021 to April 2022. Material and methods: All samples were processed within 1 hour of venipuncture. Hemolyzed samples were discarded. To separate EDTA sample tubes were taken and 3ml venous blood was collected in each tube respectively. Results: 200 samples in total were used for Westergren's (Manual Method) and ISED's ESR estimates (Automated Method). Mean age of the patients was 50.74 ± 16.84 years range from 8 to 88 years, out of total 54 % patients were male and 46 % were females, 51 % patients from OPD and 49 % were admitted patients Conclusion: The conventional Gold standard method has proven to be more efficient by CV % but p-value calculated individually as well as in all cases was statistically insignificant > 0.05%. Keywords: Automated method, Erythrocyte Sedimentation Rate, Westergren method.

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A rapid method to estimate Westergren sedimentation rates

Cited by 12 publications

References 10 publications

ICSH review of the measurement of the erythocyte sedimentation rate

ICSH review of the measurement of the erythocyte sedimentation rate

Continuous and simultaneous measurement of the biophysical properties of blood in a microfluidic environment

Comparison of Automated (ISED) ESR Estimation with Conventional (WESTERGREN) Method for Validation of Automated Results

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