A rapid method of measuring erythrocyte diameters

Hynes, Martin D.; Martin, Laurent

doi:10.1002/path.1700430111

Cited by 8 publications

(2 citation statements)

References 3 publications

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“…This was regarded by as a possible compensatory mechanism of the anaemic state. Although the shift observed by Richards and Strauss was at the abnormal plasma pH of 7.64, a similar shift of the oxygen dissociation curve to the right at the standard plasma pH of 7.4 was later reported by Dill and co-workers (1928) and by Isac, Matthes and Yamanaka (1938). Until now, a satisfactory explanation of this phcnomenon has been lacking.…”

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The Influence of Red‐Cell Thickness on the Oxygen Dissociation Curve of Blood

Valtis

Baikie

1955

Br J Haematol

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IT is known that in anaemia the oxygen dissociation curve of the blood is shifted to the right (Richards and Strauss, 1927). This was regarded by as a possible compensatory mechanism of the anaemic state. Although the shift observed by Richards and Strauss was at the abnormal plasma pH of 7.64, a similar shift of the oxygen dissociation curve to the right at the standard plasma pH of 7.4 was later reported by Dill and co-workers (1928) and by Isac, Matthes and Yamanaka (1938). Until now, a satisfactory explanation of this phcnomenon has been lacking. The shift was attributed by Dill and colleagues to the relative acidity of red cells in anaemia, which had previously been directly measured by Hampson and Maizels (1927). In a study of the oxygen dissociation curve in various human anaemias, Kennedy and Valtis (1954) found that the curve, even after correction to standard cell pH, remained shifted to the right. The only exception to this rule was the blood of patients with spherocytic anaemias in which the curve was normal in position after correction to standard cell pH. Kennedy and Valtis suspected that this difference was due to the greater thickness of the spherocytic red cells. The object of the present study was to observe the effect of alterations in red-cell shape on the blood oxygen dissociation curve and, by animal experiments, to isolate this effect from the unknown effects of anaemia. MATERIAL AND METHODSAll the observations were made on the blood of adult male guinea-pigs. Thirteen animals were used for the preliminary study of the oxygen dissociation curve of normal guinea-pigs. Spherocytosis of red cells without anaemia was produced in five animals by the intraperitoneal injection of anti-guinea-pig red-cell serum prepared in rabbits by a modification of the method of Dameshek, Schwartz and Gross (1938). Red cells thinner than normal were obtaincd from nine guinea-pigs splenectomized 2 to 3 months before. In addition, anaemias of various kinds were induced in other groups of guinea-pigs. In four animals an acute posthaemorrhagic anaemia was produced by repeated withdrawal of blood by sterile cardiac puncture; three animals were rendered anaemic by chronic lead poisoning; and in fivc animals larger doses of immune serum than had been used to produce spherocytosis alone were used to produce spherocytosis with anaemia.

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confidence: 69%

The Influence of Red‐Cell Thickness on the Oxygen Dissociation Curve of Blood

Valtis

Baikie

1955

Br J Haematol

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“…As the present series included 101 aborigines and 140 Europeans it was felt that 50 cells, measured along maximum and minimum diameters, sufficed. The mean diameter obtained in this way is possibly not as good an estimate of the "true" mean diameter of the cell as that obtained by the area method of Hynes and Martin (1936) but it permits calculation of tlie non-circularity and eccentricity of the cells.…”

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confidence: 87%

The Haematology of the Central Australian Aborigine

Casley‐Smith

1959

Aust J Exp Biol Med

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SUMMARY Total leukocyte and differential counts were performed on a group of aborigines. These lived under semi‐detribalized conditions at Haast's Bluff. A control series was taken of Europeans living at Adelaide. The total leukocyte and absolute lymphocyte counts were slightly but significantly raised in the aborigines. The eosinophil counts were markedly increased in the aborigines. Direct eosinophil counts were performed on nomads visiting Haast's Bluff. These also had greatly raised values. Many of them had positive Casoni tests. Those with positive tests had more eosinophilia than those with negative reactions. Hospital natives had fewer positive reactions.

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Die Erkrankungen des erythrocytären Systems

Heilmeyer

1942

Blutkrankheiten

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A rapid method of measuring erythrocyte diameters

Cited by 8 publications

References 3 publications

The Influence of Red‐Cell Thickness on the Oxygen Dissociation Curve of Blood

The Influence of Red‐Cell Thickness on the Oxygen Dissociation Curve of Blood

The Haematology of the Central Australian Aborigine

Die Erkrankungen des erythrocytären Systems

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