A Rapid Method of Infecting Mice and Other Laboratory Animals with Schistosoma mansoni: Subcutaneous Injection

Peters, Pierre; Warren, Kenneth S.

doi:10.2307/3277297

Cited by 151 publications

(76 citation statements)

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“…Our results are in agreement with those of PETERS & WARREN 8 , with respect to the greater effectiveness of the cercarial subcutaneous injection when compared to the percutaneous infection through the tail. However, it was evidentiated that this relation was opposite in the confrontation between subcutaneous route and the percutaneous infection through abdominal skin.…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Schistosoma mansoni: comparative evaluation of different routes of experimental infection

Tendler¹,

Rm²,

Cortês³

et al. 1985

Rev. Inst. Med. trop. S. Paulo

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SUMMARYExperiments were carried out with Sw albino mice and it was concluded that the percutaneous route via abdominal skin was significatively more efficient than tail immersion method and subcutaneous infection; the subcutaneous injection was significatively more efficient than the percutaneous infection through the tail; this latter and the intraperitoneal injection, resulted in similar infections, but were significatively less efficient than the others. Significative difference was also observed in the comparison between the subcutaneous route and percutaneous infection through ear pinna. The influence of the site of skin infection by percutaneous route was also discussed.

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Section: Discussionsupporting

confidence: 83%

“…The syringe and needle were those used for counting the cercariae. In the subcutaneous inoculation (PETERS & WAR REN 8 ) the skin in the neck region was lifted and the needle gently introduced. Mice intraperitoneally inoculated, were maintained in su pine position.…”

Section: Subcutaneous and Intraperitoneal Infectionsmentioning

confidence: 99%

Schistosoma mansoni: comparative evaluation of different routes of experimental infection

Tendler¹,

Rm²,

Cortês³

et al. 1985

Rev. Inst. Med. trop. S. Paulo

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“…The suspension was injected into the loose skin of the back of the mouse using an insulin syringe (1 cm length) [19].…”

Section: Infection Of Micementioning

confidence: 99%

A molecular Approach for Evaluation of Experimental Trials of Anti Schistosomal Vaccination in Murine Models

Etewa

Hady

Metwally

et al. 2016

Afro-Egyptian Journal of Infectious and Endemic Diseases

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“…CBA/J mice were infected s.c. at 7-8 wk of age with 35 cercariae of the Puerto Rican strain of S. mansoni (14). RAG2…”

Section: Infections With S Mansoni and Histologymentioning

confidence: 99%

CD4+ TCR Repertoire Heterogeneity inSchistosoma mansoni-Induced Granulomas

Hogan

Wang

Suresh

et al. 2002

The Journal of Immunology

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The hallmark of Schistosoma mansoni infection is the formation of liver granulomas around deposited ova. The initiation of granuloma formation is T cell-dependent since granulomas are not formed in their absence. We investigated whether a few T cells arrive to initiate the inflammatory lesion and subsequently expand locally, or whether a large repertoire of systemically activated T cells home to the delayed type hypersensitivity reaction induced by the ova. The TCR repertoire of single granulomas from the same liver were analyzed by PCR using Vβ-specific primers and CDR3 analysis. Each granuloma has a very diverse TCR repertoire indicating that most of the T cells recruited to these lesions are activated systemically. At the same time, sequence analysis of individually sized CDR3 products from single granuloma indicate that a fraction of T cells expand locally at the lesion site. Using TCR transgenic mice containing a pigeon cytochrome c-specific T cell population or lymphocytic choriomeningitis virus infection tracked with lymphocytic choriomeningitis virus-specific tetramers, we demonstrated that nonspecific T cells home to the granuloma if they are activated. However, recombinase-activating gene 2−/− pigeon cytochrome c-specific TCR transgenic mice fail to form granulomas in response to S. mansoni ova even after T cell activation, suggesting a requirement for egg-specific T cells in the initiation of these inflammatory lesions. Understanding the mechanism of T cell recruitment into granulomas has important implications for the rational design of immunotherapies for granulomatous diseases.

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A Rapid Method of Infecting Mice and Other Laboratory Animals with Schistosoma mansoni: Subcutaneous Injection

Cited by 151 publications

References 0 publications

Schistosoma mansoni: comparative evaluation of different routes of experimental infection

Schistosoma mansoni: comparative evaluation of different routes of experimental infection

A molecular Approach for Evaluation of Experimental Trials of Anti Schistosomal Vaccination in Murine Models

CD4+ TCR Repertoire Heterogeneity inSchistosoma mansoni-Induced Granulomas

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