A rapid method of fruit cell isolation for cell size and shape measurements

Abstract

Background: Cell size is a structural component of fleshy fruit, contributing to important traits such as fruit size and texture. There are currently a number of methods for measuring cell size; most rely either on tissue sectioning or digestion of the tissue with cell wall degrading enzymes or chemicals to release single cells. Neither of these approaches is ideal for assaying large fruit numbers as both require a considerable time to prepare the tissue, with current methods of cell wall digestions taking 24 to 48 hours. Additionally, sectioning can lead to a measurement of a plane that does not represent the widest point of the cell.

Adjustment of milling, mash electroporation and pressing for the development of a PEF assisted juice production in industrial scale

“…Values for apple mash redrawn from Schobinger (2001) are given as a reference (ref) for comparison.for carrot cells and byBain and Robertsen (1951) andMcAtee et al (2009) for apple cells.…”

High performance liquid chromatography method for the detection of released purinergic and biogenic amine signaling molecules from in vitro ileum tissue

“…The tissue area was calculated by a pixel counting algorithm which has been widely used 34–36. Photographic images were taken of the modified tissue well plate in which the tissue samples were placed and the image was processed using IMAGE J software 37 to accurately measure the area of the pinned out isolated ileum tissue sections in pixel numbers, which was converted to cm 2 . The concentrations of the signaling molecules from the chromatographic assay (obtained from calibration curves of the analytes) was then normalized to the area of the tissue.…”

Organ-wide and ploidy-dependent regulations both contribute to cell size determination: evidence from a computational model of tomato fruit

“…Indeed, mechanical constraints acting on real tissues as well as vascularisation can largely modify cell shape, resulting in elongated or multi-lobed cells (Ivakov and Persson, 2013). Thus, if the orientation of 2D slices can potentially affect the resulting cell area estimation, possible differences between in-vivo tissues and dissociated cells should also be systematically checked (McAtee et al 2009). The size of the dataset is also important to correctly characterized the expected distribution shape.…”