A rapid method of determining growth characteristics of plant cell populations in batch suspension culture

Gilissen, L.J.W.J.; Cate, Ch. H. Hänisch Ten; Keen, B.

doi:10.1007/bf00269148

Cited by 30 publications

(6 citation statements)

References 9 publications

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“…The growth of cultures was monitored by fresh weight (determined from vacuum-filtered cells), protein (measured by the micro-assay according to Biorad) and packed cell volume (determined as previously described [10]). …”

Section: Methodsmentioning

confidence: 99%

Peroxidase production by cell suspension and hairy root cultures of horseradish (Armoracia rusticana)

1990

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The potentml of cell suspension and Imiry root' cultures of horseradish for production of pero~ddase was assessed and the factors limiting productmn in culture were examined. Peroxidase activity is closely related to growth in both cell suspension cultures and 'hairy root' cultures of horseradish. Under the growth conditions used in the present study however, cell suspensmn cultures produce twice the total activity of 'han-y root' cultures, and up to 8% of tins activity is released into the culture filtrate at high specific activity. The growth and peroxidase activity of cell suspension cultures are limited by both phosphate and sucrose and the rate of oxygenatmn of the culture is critical for maximising growth rate, biomass accumulation and peroxidase activity. The isoelectric focussing banding pattern of peroxidase from the 'hairy root' cultures was identical with commercmlly available horseradish permddase whilst that from cell suspension cultures differed with an additional two bands at high pI.

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Section: Methodsmentioning

confidence: 99%

Peroxidase production by cell suspension and hairy root cultures of horseradish (Armoracia rusticana)

1990

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“…Every 2 days, the flasks were briefly removed from the orbital shaker, the cells allowed to settle for 10 min and the volume of settled cells (SCV) recorded [7]. From these measurements, the specific growth rate of the cultures was determined.…”

Section: Experimental Conditionsmentioning

confidence: 99%

Influence of exogenous L-proline on embryogenic cultures of larch (Larix leptoeuropaeaDengler), sitka spruce (Picea sitchensis(Bong.) Carr.) and oak (Quercus roburL.) subjected to cold and salt stress

Gleeson

Lelu‐Walter²,

Parkinson

2004

Ann. For. Sci.

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NoteInfluence of exogenous L-proline on embryogenic cultures of larch (Larix leptoeuropaea Dengler), sitka spruce (Picea sitchensis (Bong.) Carr.) and oak (Quercus robur L.) subjected to cold and salt stress Deirdre GLEESON a Abstract -The effect of exogenous L-proline (1 mM, 10 mM, and 100 mM) on embryogenic cultures of larch, sitka spruce and oak subjected to environmental stresses was examined. Low temperature (4°C) completely inhibited growth of the cultures and this was partially alleviated by the addition of proline. Our studies show that not only can cultures survive low temperatures, but are capable of active growth while the cold stress is still being applied. Growth was inversely related to [NaCl] with complete inhibition at 200 mM. Proline stimulated growth at all concentrations tested permitting growth with 200 mM NaCl even at low (1 mM) proline concentrations. Release of internal cellular potassium was inversely related to freezing temperature and this release was reduced by exogenous proline. These results for cultures of forest species are consistent with findings previously reported for deciduous herbaceous angiosperms and suggest that proline may have a role in protection of forest species from environmental stresses. trees / chilling / environmental stress / toleranceRésumé -Effet de la L-proline apportée de manière exogène à des cultures embryogènes, de mélèze hybride (Larix leptoeuropaea Dengler), d'épicéa de sitka (Picea sitchensis Bong.) et de chêne (Quercus robur L.), soumises à des stress au froid et salin. Des cultures embryogènes de mélèze hybride, d'épicéa de sitka et de chêne ont été soumises à différentes conditions de culture et leur croissance étudiée en fonction de l'ajout de L-proline au milieu de culture (1 mM, 10 mM, et 100 mM). Si des températures basses (4°C) inhibent totalement la croissance des cultures, celle-ci redevient partiellement normale en présence de proline. Nos résultats montrent que les cultures non seulement survivent à de basses températures mais sont aussi capables de croître activement au cours de la durée d'application du froid. De même, la croissance est inversement corrélée à la concentration en sel avec sa complète inhibition en présence de 200 mM de NaCl. L'addition de L-proline au milieu de culture (quelles que soient les concentrations testées) stimule la croissance des cultures, même en présence de 200 mM de NaCl. La libération de potassium intracellulaire est inversement corrélée à la température de congélation, libération qui est réduite en présence de proline exogène. Ces résultats, obtenus pour des cultures d'espèces forestières, sont en accord avec ceux précédemment rapportés pour des espèces herbacées. Ils suggèrent le rôle potentiel de la proline dans la protection de ces espèces forestières soumises à des stress abiotiques.arbre / froid / stress abiotique / tolérance

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“…Weekly, 9 ml of cells of a 7 days old growing suspension culture were transferred to 40 ml fresh medium in a 250-nil Erlenmeyer flask closed with a cotton plug. The flasks were kept on an orbital shaker at 20°C under continuous 10 fimol m"ŝ "' FAR fluorescent light (Philips TLD 36 W/33), Growdi of tbe cells was measured after Gilissen et al, (1983),…”

Section: Plant Ttnaterialmentioning

confidence: 99%

Respiratory energy requirements and rate of protein turnover in vivo determined by the use of an inhibitor of protein synthesis and a probe to assess its effect

Bouma

Visser²,

Janssen

et al. 1994

Physiologia Plantarum

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Protein turnover is generally regarded as a major maintenance process, but experimental evidence to support this contention is scarce. Here we quantify the component of dark respiration rate associated with overall protein turnover of tissues in vivo. The effect of an inhibitor of cytosolic protein synthesis (cycloheximide, CHM) on dark respiration was tested on a cell suspension from potato (Solanum tuberosum L.) and quantified on leaf discs of expanding and full‐grown primary leaves of bean (Phaseolus vulgaris L.). The in vivo effect of CHM on protein biosynthesis was assessed by monitoring the inhibition of the induction of the ethylene‐forming enzyme (EFE) activity. The present method yields the energy costs of turnover of the total pool of proteins irrespective of their individual turnover rates. Average turnover rates were derived from the respiratory costs and the specific costs for turnover. Inhibition of respiration by CHM was readily detectable in growing‐cell suspensions and discs of expanding leaves, The derived respiratory costs of protein turnover in expanding leaves were maximally 17–37% of total respiration. Turnover costs in full‐grown primary leaves of bean amounted to 17–21% of total dark respiration. The maximum degradation constants (i.e. Kd‐values) derived for growing and full‐grown leaves were up to 2.42 × 10−6 and 1.12 × l0−6 s−1, respectively.

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A rapid method of determining growth characteristics of plant cell populations in batch suspension culture

Cited by 30 publications

References 9 publications

Peroxidase production by cell suspension and hairy root cultures of horseradish (Armoracia rusticana)

Peroxidase production by cell suspension and hairy root cultures of horseradish (Armoracia rusticana)

Influence of exogenous L-proline on embryogenic cultures of larch (Larix leptoeuropaeaDengler), sitka spruce (Picea sitchensis(Bong.) Carr.) and oak (Quercus roburL.) subjected to cold and salt stress

Respiratory energy requirements and rate of protein turnover in vivo determined by the use of an inhibitor of protein synthesis and a probe to assess its effect

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