A rapid method for the separation of vitamin D and its metabolites by ultra-high performance supercritical fluid chromatography–mass spectrometry

Determination of fat‐ and water‐soluble vitamins by supercritical fluid chromatography: A review

“…SFC-TOF-MS was applied by Jumaah et al [81] to separate nine vitamin D metabolites on Acquity UPC 2 1-AA at the temperature of 35 • C. As a mobile phase, CO 2 modified by methanol with the addition of 0.2% of ammonium formate was used. The applied method resulted in all compounds separation to the baseline.…”

Theory and Practice of UHPLC and UHPLC–MS

“…However, SFCeMS is still much less widely utilized compared with HPLC-MS, although a significant increase in the number of published papers has occurred very recently . The main purposes to employ SFC in bioanalysis were: (1) to analyze groups of compounds that are challenging in RP-HPLC, such as antibiotics (Yuan et al, 2016;Eom et al, 2016), lipids, and fat-soluble vitamins (Pilarová et al, 2016;Jumaah et al, 2016;Taguchi et al, 2014), (2) to develop enantioselective methods for the quantification of drugs and their chiral metabolites , and finally, (3) to obtain different selectivity for the analysis of metabolites (Sen et al, 2016;Spaggiari et al, 2014). Chromatograms exemplifying the benefits of UHPSFCeMS in analysis of structural isomers of vitamin E in human serum are shown in Fig.…”

“…Because SFCeMS is quite a new approach in bioanalysis, many studies focus on detailed optimization of the individual parameters of SFCeMS, including ion source set-up, mobile phase composition, make-up solvent composition, temperature, and BPR pressure (Jumaah et al, 2016;Pilarová et al, 2016;Sen et al, 2016;Yuan et al, 2016;Yang et al, 2016;Spaggiari et al, 2014). Matrix effects are an important obstacle of HPLCeMS and their determination is now obligatory in bioanalytical methods.…”

Investigation of chromatographic peak broadening in supercritical fluid chromatography/atmospheric pressure chemical ionization mass spectrometry

“…ESI, enabling efficient ionization of moderate to high polarity compounds, is now used in most of SFC/MS analyses. Nevertheless, separation of low to moderate polarity substances remains the important target of SFC application in food analysis, metabolomics, clinical analysis and pharmaceutical industry [3][4][5][6][7][8][9][10][11][12]. Although APCI can be more favorable for low to moderate polarity compounds than ESI it occupies only a small share of SFC/MS publications (21% out of 157 references on Web of Science published in 2000-2018 [2]).…”