A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts

Suberu, John O.; Song, Lijiang; Slade, Susan E.; Sullivan, Neil F.; Barker, Guy C.; Lapkin, Alexei

doi:10.1016/j.jpba.2013.06.025

Cited by 44 publications

(34 citation statements)

References 42 publications

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“…Surprisingly, S rootstocks stimulated production of AN in G scions where neither AN nor GLTs were previously detectable. Using the more sensitive method of HPLC tandem mass spectrometry, others (Suberu et al 2013) also reported possible trace levels of AN in nG (analysis was inconclusive), so G may have weak AN production capability. Together these results demonstrated that the S rootstock stimulated production of DHAA, AN, and dAN in both S and 15 scions.…”

Section: Rootstock Effects On Artemisinic Metabolitesmentioning

confidence: 99%

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Root regulation of artemisinin production in Artemisia annua: trichome and metabolite evidence

Wang

Towler

Weathers

2016

Planta

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Roots of plants with high artemisinin-producing leaves increased leaf production of artemisinin in low-producing plants and vice versa indicating roots are involved in controlling artemisinin biosynthesis in shoots. The anti-malarial sesquiterpene, artemisinin, is produced and stored in glandular trichomes (GLTs) of Artemisia annua. Evidence suggested roots, which produce no significant artemisinin nor precursor compounds, regulate production of artemisinin biosynthesis in the leaves. Using grafting, we studied the relationship between rootstock and scion by measuring GLTs and five artemisinic metabolites (artemisinin, deoxyartemisinin, dihydroartemisinic acid, artemisinic acid, arteannuin B) in scions of ungrafted, self-grafted, and cross-grafted plants among three cultivars: S and 15 both having GLTs with artemisinin at 1.49 and 0.57 %, respectively, and G producing neither GLTs nor detectable artemisinin. All artemisinin-producing self-grafts, e.g., S/S (scion/rootstock) and 15/15, produced more artemisinin than ungrafted plants, likely from grafting stress. S/S grafts also produced more GLTs. The 15/S grafts produced more artemisinin than S/15, suggesting rootstocks from high producing S plants stimulated artemisinin production in 15 scions. S/15 grafts yielded less artemisinin than S/S, but more than either 15/15 or ungrafted n15 and nS; S/15 grafts also had a lower density of GLTs than S/S, suggesting rootstock inhibition of the scion. The S rootstock induced trace artemisinin production in G scions, but did not induce GLT formation in G/S grafts. Different grafts exhibited different trichome morphologies and effects on artemisinic pathway flux. This study provides new information regarding the role of roots in GLT development and artemisinin production in this important medicinal plant.

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Section: Rootstock Effects On Artemisinic Metabolitesmentioning

confidence: 99%

“…Of more than 40 flavonoids found in the plant (Ferreira et al 2010), at least ten have some weak antimalarial activity (Lehane and Saliba 2008;Ganesh et al 2012;Suberu et al 2013), and some work synergistically with AN against falciparum malaria (Liu et al 1992).…”

Section: Rootstock Effects On Flavonoidsmentioning

confidence: 99%

Root regulation of artemisinin production in Artemisia annua: trichome and metabolite evidence

Wang

Towler

Weathers

2016

Planta

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“…There is extensive and continuing interest in developing attractive and fast methods of studying ART drug. Current methods developed for the analysis of ART include high performance or ultra-high performance liquid chromatography with mass spectrometry 9 . ART is an electroactive molecule and can be analysed through electrochemical means.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Mazzochette

Newton

2017

Anal. Methods

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“…High performance liquid chromatography (HPLC) is considered as a robust method in numerous applications of qualitative and quantitative analyses of TCM for its easy operation, high accuracy and wide suitability. In quantification, determination of a group of principal active constituents with similar or different structures in one medicinal plant has been widely implemented [22], [23], [24], [25].…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Wild Paris Based on Near Infrared Spectroscopy and High Performance Liquid Chromatography Combined with Multivariate Analysis

Zhao

Zhang

Tianjun³

et al. 2014

PLoS ONE

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Different geographical origins and species of Paris obtained from southwestern China were discriminated by near infrared (NIR) spectroscopy and high performance liquid chromatography (HPLC) combined with multivariate analysis. The NIR parameter settings were scanning (64 times), resolution (4 cm−1), scanning range (10000 cm−1∼4000 cm−1) and parallel collection (3 times). NIR spectrum was optimized by TQ 8.6 software, and the ranges 7455∼6852 cm−1 and 5973∼4007 cm−1 were selected according to the spectrum standard deviation. The contents of polyphyllin I, polyphyllin II, polyphyllin VI, and polyphyllin VII and total steroid saponins were detected by HPLC. The contents of chemical components data matrix and spectrum data matrix were integrated and analyzed by partial least squares discriminant analysis (PLS-DA). From the PLS-DA model of NIR spectrum, Paris samples were separated into three groups according to the different geographical origins. The R2X and Q2Y described accumulative contribution rates were 99.50% and 94.03% of the total variance, respectively. The PLS-DA model according to 12 species of Paris described 99.62% of the variation in X and predicted 95.23% in Y. The results of the contents of chemical components described differences among collections quantitatively. A multivariate statistical model of PLS-DA showed geographical origins of Paris had a much greater influence on Paris compared with species. NIR and HPLC combined with multivariate analysis could discriminate different geographical origins and different species. The quality of Paris showed regional dependence.

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A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts

Cited by 44 publications

References 42 publications

Root regulation of artemisinin production in Artemisia annua: trichome and metabolite evidence

Root regulation of artemisinin production in Artemisia annua: trichome and metabolite evidence

Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Discrimination of Wild Paris Based on Near Infrared Spectroscopy and High Performance Liquid Chromatography Combined with Multivariate Analysis

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