A Rapid Method for the Determination of Fucoxanthin in Diatom

Wang, Lijuan; Fan, Yong; Parsons, Ronald L.; Hu, Guang-Rong; Zhang, Peiyu; Li, Fuli

doi:10.3390/md16010033

Cited by 66 publications

(69 citation statements)

References 30 publications

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“…The spectra of the supernatant from 350 to 750 nm were recorded using a Perkin Elmer spectrophotometer (PTP-6 Peltier System). Chlorophyll a and c concentrations were calculated according to (Ritchie, 2006) and fucoxanthin concentration according to (Wang et al , 2018). For extended darkness, 24 hours after induction cells were transferred to darkness for 21 days.…”

Section: Methodsmentioning

confidence: 99%

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Avilán

Lebrun

Puppo

et al. 2020

Preprint

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Chloroplasts retain elements of a bacterial stress response pathway that is mediated by the signalling nucleotides guanosine penta-and tetraphosphate, or (p)ppGpp. In the model flowering plant Arabidopsis, ppGpp acts as a potent regulator of plastid gene expression and influences photosynthesis, plant growth and development.However, little is known about ppGpp metabolism or its evolution in other photosynthetic eukaryotes.• Here, we studied the function of ppGpp in the diatom P. tricornutum using transgenic lines containing an inducible system for ppGpp accumulation. We used these lines to investigate the effects of ppGpp on growth, photosynthesis, lipid metabolism and protein expression.• We demonstrate that ppGpp accumulation reduces photosynthetic capacity and promotes a quiescent-like state with reduced proliferation and ageing. Strikingly, using non-targeted proteomics, we discovered that ppGpp accumulation also leads to the coordinated upregulation of a protein protection response in multiple cellular compartments.• Our findings highlight the importance of ppGpp as a fundamental regulator of chloroplast function across different domains of life, and lead to new questions about the molecular mechanisms and roles of (p)ppGpp signalling in photosynthetic eukaryotes.

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Section: Methodsmentioning

confidence: 99%

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Avilán

Lebrun

Puppo

et al. 2020

Preprint

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“…To obtain robust data, spectrophotometric methods require the previous adjustment of the quantification protocol to the specific experimental conditions: establish the correct titration of the sample, adapt reading parameters and equations to different solvents, and determine adequate negative and positive controls [44,69,70]. The specific quantification of fucoxanthin by spectrophotometry has been scarcely developed [44,71]. Nevertheless, they have been described as easier, faster and cheaper than LC.…”

Section: Quantification and Identification Of Fucoxanthinmentioning

confidence: 99%

“…A study analyzed the content of fucoxanthin extracts using a microplate reader. This technique allowed results with lower standard errors (<5%) than HPLC to be obtained [71]. Spectrophotometric methods are cheaper since they reduce experimental times, do not require highly trained personnel or equipment for their development, nor do they use a high volume of organic solvents, so they can be also considered as greener techniques than those that are LC-based.…”

Section: Quantification and Identification Of Fucoxanthinmentioning

confidence: 99%

Scientific Approaches on Extraction, Purification and Stability for the Commercialization of Fucoxanthin Recovered from Brown Algae

Lourenço-Lopes

García-Oliveira

Carpena

et al. 2020

Foods

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The scientific community has corroborated the numerous beneficial activities of fucoxanthin, such as its antioxidant, anti-inflammatory, anticancer or neuroprotective effects, among others. These properties have attracted the attention of nutraceutical, cosmetic and pharmacological industries, giving rise to various possible applications. Fucoxanthin may be chemically produced, but the extraction from natural sources is considered more cost-effective, efficient and eco-friendly. Thus, identifying suitable sources of this compound and giving a general overview of efficient extraction, quantification, purification and stabilization studies is of great importance for the future production and commercialization of fucoxanthin. The scientific research showed that most of the studies are performed using conventional techniques, but non-conventional techniques begin to gain popularity in the recovery of this compound. High Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance (NMR) and spectroscopy techniques have been employed in the quantification and identification of fucoxanthin. The further purification of extracts has been mainly accomplished using purification columns. Finally, the stability of fucoxanthin has been assessed as a free molecule, in an emulsion, or encapsulated to identify the variables that might affect its further industrial application.

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“…Fukosantin telah banyak diteliti pemanfaatannya sebagai salah satu suplemen makanan yang aman dikonsumsi oleh manusia. Beberapa manfaat dari fukosantin yaitu digunakan sebagai anti-inflamasi, anti-tumor, anti-obesitas, anti-diabetes, anti-malaria dan berbagai macam aktivitas fisiologi lainnya (Li et al, 2018).…”

Section: Pendahuluanunclassified

Kandungan Klorofil dan Fukosantin serta Pertumbuhan Skeletonema costatum pada Pemberian Spektrum Cahaya Yang Berbeda

et al. 2019

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Skeletonema costatum mengandung klorofil-a, klorofil-c, dan fukosantin yang menyebabkan selnya berwarna hijau kecoklatan. Klorofil dan fukosantin memiliki berbagai manfaat, salah satunya dalam bidang kesehatan sebagai anti-bakteri, anti-oksidan, anti-inflamasi, anti-obesitas, anti-diabetes. Cahaya merupakan faktor lingkungan yang dapat mempengaruhi pertumbuhan dan kandungan pigmen pada mikroalga. Penelitian ini bertujuan untuk menentukan spektrum cahaya yang memiliki pengaruh terhadap pertumbuhan, kandungan klorofil dan fukosantin S. costatum. Penelitian ini dilakukan pada bulan Januari-Maret 2018 di Laboratorium Biologi, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro dan pengujian laboratoris di Laboratorium BPIK Srondol, Semarang. Metode yang digunakan adalah metode eksperimental laboratoris. Diatom S. costatum dikultivasi dengan tiga spektrum cahaya yang berbeda yaitu putih, biru, dan merah. Pertumbuhan sel S. costatum diamati sampai 2 x 24 jam kemudian dipanen untuk perhitungan biomassanya. Biomassa kering hasil kultivasi diekstraksi menggunakan metanol. Kadar pigmen ekstrak metanol dianalisis menggunakan spektrofotometer UV-Vis dan identifikasi pigmen dengan uji Kromatografi Lapis Tipis (KLT). Hasil penelitian menunjukkan bahwa perlakuan cahaya putih secara signifikan meningkatkan kandungan klorofi- a S. costatum dibandingkan spektrum cahaya merah, namun tidak berbeda nyata terhadap spektrum cahaya biru. Pertumbuhan, kandungan klorofil-c dan fukosantin S. costatum pada pemberian spektrum cahaya yang berbeda tidak menunjukkan perbedaan nyata. Skeletonema costatum contains chlorophyll-a, chlorophyll-c, and fucoxanthin giving to its cells. Chlorophyll and fucoxanthin have various benefits, e.g. in the medicine field as anti-bacterial, anti-oxidant, anti-inflammatory, anti-obesity, and anti-diabetes. Light is one of the environmental factor that affects the growth and pigment content of microalgae. This study aims to determine the spectrum of light that influences growth, chlorophyll content and fucoxanthin of S. costatum. This research was conducted in January-March 2018 at the Biology Laboratory, Faculty of Fisheries and Marine Sciences, Diponegoro University and laboratory testing at the BPIK Srondol Laboratory, Semarang. The method used was a laboratory experimental method. Diatom S. costatum was cultivated with three different spectrums of light (white, blue, and red). Growth of S. costatum cells was observed up to 2x24 hours and then harvested for biomass calculations. Dry biomass was extracted using methanol. Pigment content of The S. costatum methanol extract was analyzed using UV-Vis spectrophotometer and pigments identification using Thin Layer Chromatography (TLC). The results showed that the chlorophyll content of S. costatum under white light spectrum was significantly higher from the red light spectrum, but not significantly different from blue light spectrum. Growth, chlorophyll-c and fucoxanthin content of S. costatum didn’t show significant differences under different light spectra.

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A Rapid Method for the Determination of Fucoxanthin in Diatom

Cited by 66 publications

References 30 publications

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Scientific Approaches on Extraction, Purification and Stability for the Commercialization of Fucoxanthin Recovered from Brown Algae

Kandungan Klorofil dan Fukosantin serta Pertumbuhan Skeletonema costatum pada Pemberian Spektrum Cahaya Yang Berbeda

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