A rapid method for the sequential separation of polonium, plutonium, americium and uranium in drinking water

Lemons, B. G.; Khaing, H.; Ward, Anderson L.; Thakur, P.

doi:10.1016/j.apradiso.2018.02.008

Cited by 16 publications

(2 citation statements)

References 18 publications

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“…The complete MinION data can be further analyzed and polished after the entire sequencing run to obtain accurate, whole genomes that provide detailed data on subtyping, virulence genes, antimicrobial resistance genes, and other genetic characteristics. Same-day detection of antimicrobial resistance genes with 99.75% accuracy (with polishing) after enriching for plasmid DNA and MinION sequencing has been recently demonstrated 47 . Here, we also demonstrate the potential of the un-polished, MinION-only results for rapid phylogenetic inference with identical topology as compared to the reference published dataset after only 30 mins of sequencing, with consistent results at all other analyzed time points.…”

Section: Discussionmentioning

confidence: 99%

Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology

Taylor

Volkening²,

DeJesus

et al. 2019

Preprint

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18United States public health agencies are focusing on next-generation sequencing (NGS) to 19 quickly identify and characterize foodborne pathogens. Here, the MinION nanopore, long-read 20 sequencer was used to simultaneously sequence the entire chromosome and plasmids of Salmonella 21 enterica subsp. enterica serovar Bareilly and Escherichia coli O157:H7. A rapid, random sequencing 22 approach, coupled with de novo genome assembly within a customized data analysis workflow, that can 23 resolve highly-repetitive genomic regions, was developed. In sequencing runs, as short as four hours, 24 using nanopore data alone, full-length genomes were obtained with an average identity of 99.87% for 25Salmonella Bareilly and 99.89% for E. coli in comparison to the respective MiSeq references. These long-26 read assemblies provided information on serotype, virulence factors, and antimicrobial resistance genes. 27Using a custom-developed, SNP-selection workflow, the potential of the nanopore-only assemblies 28 (after only 30 minutes of sequencing) for rapid phylogenetic inference, with identical topology 29 compared to the published dataset, was demonstrated. To achieve maximum quality assemblies, the 30 developed bioinformatics workflow employed additional polishing steps to correct the systematic errors 31 produced by the nanopore-only assemblies. Nanopore sequencing provided a shorter (10 hours library 32 preparation and sequencing) turnaround time compared to other NGS technologies. 33 34 Keywords: MinION, complete genome sequencing, plasmids, foodborne pathogen, long-reads, assembly 35 workflow, tracing. 36 basecalled using Albacore (ONT v 2.0.2b) and subsampled for assembly using Filtlong (v.0.2.0) 24 to a 132

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Section: Discussionmentioning

confidence: 99%

Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology

Taylor

Volkening²,

DeJesus

et al. 2019

Preprint

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“…210 Po 4+ adsorbed to extraction chromatography resin together with the transuranium isotope ions can be removed before eluting Pu, Am and Cm. For example, 210 Po can be removed using 8 M HNO 3 from TRU column before eluting Am + Cm [51] and also with 8 M HNO 3 from TEVA column [52]. It is also possible to elute Pu from a TRU column with a solution containing the reducing reagent TiCl 3 , instead of more commonly used ammonium oxalate solution, for keeping any residual tetravalent 210 Po 4+ in the resin column while eluting trivalent Pu 3+ [53].…”

Section: Future Aims For the Radioanalytical Developmentmentioning

confidence: 99%

Analyzing alpha emitting isotopes of Pu, Am and Cm from NPP water samples: an intercomparison of Nordic radiochemical laboratories

Salminen‐Paatero

Hou

Olszewski

et al. 2021

J Radioanal Nucl Chem

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Radioanalytical methods for the determination of isotopes of Pu, Am and Cm in water samples from nuclear power plants were compared and further developed in a Nordic project (Optimethod) through two intercomparison exercises among Nordic laboratories. With this intercomparison, the analytical performance of some laboratories was improved by modification of the analytical method and adopting new techniques. The obtained results from the two intercomparisons for alpha emitting transuranium isotopes are presented, and the lessons learnt from these intercomparison exercises are discussed.

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Synthesis of thiol-functionalized hydrotalcite and its application for adsorption of uranium (VI)

Yin

et al. 2018

J Radioanal Nucl Chem

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A rapid method for the sequential separation of polonium, plutonium, americium and uranium in drinking water

Cited by 16 publications

References 18 publications

Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology

Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology

Analyzing alpha emitting isotopes of Pu, Am and Cm from NPP water samples: an intercomparison of Nordic radiochemical laboratories

Synthesis of thiol-functionalized hydrotalcite and its application for adsorption of uranium (VI)

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