A Rapid Method for Inoculating Pea Seedlings with Fusarium oxysporum f. sp. pisi

Haglund, W. A.

doi:10.1094/pd-73-0457

Cited by 32 publications

(20 citation statements)

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“…Techniques to screen peas for resistance to wilt include : 1) pruning roots while submersed in a conidial suspension (Wells et al ., 1949 ;Haglund, 1989) ; 2) pouring mycelial fragments and conidia into a trough adjacent to seedling roots growing in sand (Armstrong & Armstrong, 1974) ; and 3) pouring conidia and hyphal fragments into holes punched into potting soil with a pointed rod to wound roots (Doling, 1963) . In Washington state, resistance to race 1 wilt is determined under field conditions at Pullman, Washington, where a race 1 field nursery was established by Dr. F. J. Muehlbauer, USDA/ARS .…”

Section: Common Root Rot Of Peasmentioning

confidence: 99%

Screening techniques and sources of resistance to root rots and wilts in cool season food legumes

et al. 1993

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Section: Common Root Rot Of Peasmentioning

confidence: 99%

Screening techniques and sources of resistance to root rots and wilts in cool season food legumes

et al. 1993

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“…Selected isolates of the FOC were tested for pathogenicity by a slight modification of the root dipping method described by Haglund [27]. Pea seeds (cv.…”

Section: Methodsmentioning

confidence: 99%

Population structure and pathogenicity of members of the Fusarium oxysporum complex isolated from soil and root necrosis of pea (Pisum sativum L.)

Skovgaard

Bødker²,

Rosendahl

2002

FEMS Microbiology Ecology

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Forty-nine strains of the Fusarium oxysporum complex were isolated from five different sample locations within two neighboring pea fields. Of these, 39 strains were isolated from soil and 10 from pea plants showing symptoms of root rot. Twenty-eight of the isolates were tested for pathogenicity towards pea. Based on percentage discoloration of the roots and stem base, the isolates were divided into three groups: seven strains were pathogenic, 14 strains were weakly pathogenic, and seven strains were non-pathogenic towards pea. To assess the genetic relatedness of all 49 strains, gene genealogies were constructed from aligned DNA sequences from part of the translation elongation factor, nitrate reductase, beta tubulin, and mitochondrial small subunit rDNA. Maximum parsimony analysis of the combined data set yielded a single most-parsimonious tree containing three strongly supported clades which may represent cryptic species. No correlation was observed between the multigene phylogeny and pathogenicity toward pea, strain geographic origin and substrate (soil or plant) from which the strains were isolated. Strains that were non-pathogenic, weakly pathogenic or pathogenic sometimes shared the same multilocus genotype. These results suggest that strains pathogenic and putatively non-pathogenic to pea are very closely related genetically.

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“…Techniques for inoculation have been reviewed previously (Haglund, 1989) and involve removing approximately one-third of the root system from 7-10-day-old seedlings and submerging the rest of the root system in inoculum adjusted to 1 × 10 6 spores ml −1 . Methods for inoculum production can be found in Haglund (1989) and McPhee et al (1999). The difference in techniques is based on whether the roots are trimmed while submerged in the inoculum (dipand-cut) or prior to submersion (cut-and-dip).…”

Section: Greenhouse and Laboratory Screeningmentioning

confidence: 99%

Screening techniques and sources of resistance to root diseases in cool season food legumes

et al. 2006

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Soil-borne fungal diseases are among the most important factors, limiting the yield of grain legumes in many countries worldwide. Root rot, caused by Aphanomyces euteiches, Rhizoctonia solani, Fusarium solani and wilt, caused by several formae speciales of Fusarium oxysporum are the most destructive soil-borne diseases of pea, chickpea, lentil, fababean and lupin. The most effective control of these diseases is achieved through the use of resistant varieties. In this paper, recent advances in conventional and innovative screening methods for disease resistance are presented. Many grain legume accessions, which are maintained in national and international germplasm collections, have been evaluated for disease resistance and numerous resistant varieties have been released following incorporation of identified resistance genes from these sources. Recent identification of molecular markers tightly linked to resistance genes has greatly enhanced breeding programs by making marker assisted selection (MAS) possible and allowing the development of varieties with multiple disease resistance. Progress in the understanding of the biology of soil-borne fungal pathogens of grain legumes is also reviewed with particular reference to the genetic structure of their populations, diagnosis and host-pathogen interaction.

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A Rapid Method for Inoculating Pea Seedlings with Fusarium oxysporum f. sp. pisi

Cited by 32 publications

References 0 publications

Screening techniques and sources of resistance to root rots and wilts in cool season food legumes

Screening techniques and sources of resistance to root rots and wilts in cool season food legumes

Population structure and pathogenicity of members of the Fusarium oxysporum complex isolated from soil and root necrosis of pea (Pisum sativum L.)

Screening techniques and sources of resistance to root diseases in cool season food legumes

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