A rapid method for identification of Mycobacterium species by polyacrylamide gel electrophoresis of soluble cell proteins

Abstract

Summary. The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of an easily and rapidly prepared soluble protein fraction were used in conjunction with conventional techniques to identify different strains of Mycobacterium tuberculosis, M. bovis, M . bovis BCG, M . africanum, M. avium, M . kansasii, M . marinurn, M . gastri, M . simiae and M . malmoense. Complete concordance of results from both methods was obtained with all species except those of the M. tuberculosis complex. With the SDS-PAGE technique, all strains of the M. tuberculosis complex were recognised as belonging to one species. By visual analysis of the SDS-PAGE polypeptide profiles, only minor differences between strains of the same species were seen and each species showed a characteristic polypeptide profile. Quantitation of the data by calculation of the Dice coefficient of similarity of the band positions obtained by densitometry indicated that the similarity between different strains of one species was 90-100% and the similarity between the species was in the range 30-45%. The results indicate that SDS-PAGE is a simple and rapid method for identifying mycobacterial strains.

Biochemical properties and whole‐cell protein profiles of group G streptococci isolated from dogs

“…Whole cell-protein patterns have been used to type (Delmett et al Want 1989, 1992) and differentiate bacterial species (De Jong et al 1991;Merquior et al 1994). In this study, however, they did not provide the basis for the establishment of a typing scheme.…”

Protein synthesis patterns ofParacoccidioides brasiliensisisolates in stage-specific forms and during cellular differentiation

“…The characterization of isolates through protein profiles has been described in several micro-organisms [11,12,14,24,25]. The clustering tendency evidenced for isolates in the yeast phase suggests the discriminatory capacity of the technique in characterizing the isolates.…”

“…Stage specific proteins, like a 70 kDa species, are inhibited during yeast to mycelium phase transition. The analysis of protein patterns in specific phases of growth may constitute a way of characterizing P. brasiliensis isolates, as has been found with other pathogens [11][12][13][14], and in addition may contribute to basic biological knowledge of the fungus. The identification of molecular alterations during the initial stages of cellular differentiation may be important in the characterization of proteins that could be related to dimorphism.…”

Simultaneous detection and strain differentiation of Mycobacterium tuberculosis complex in paraffin wax embedded tissues and in stained microscopic preparations

“…All strains were identified in our laboratory by using AccuProbe (Gen-Probe Inc, San Diego, California, USA), as described previously. [13][14][15] DNA EXTRACTION FROM PARAFFIN WAX…”