A rapid method for determination of l-lactic acid in real samples by amperometric biosensor utilizing nanocomposite

Monošík, Rastislav; Stredansky, Matuš; Greif, Gabriel; Šturdı́k, Ernest

doi:10.1016/j.foodcont.2011.07.021

Cited by 52 publications

(26 citation statements)

References 33 publications

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“…By following the above approaches, has already demonstrated how the biocompatibility property of CS guarantees the improvement of the limit of detection and enzyme stability (Cruz et al 2000). Several authors mixed CS with MWCNTs to enhance electrochemical behavior of electrochemical biosensors regarding electrical conductivity and electrocatalytic activity properties (Cui et al 2007;Chen et al 2004;Monošík et al 2012;Zhou and Hartmann 2012) Encouraged by the improvement of the stability, reproducibility and repeatability of the lactate electrochemical biosensor, this study depicts the manufacture of a bio-enzymatic biosensor using both Horseradish Peroxidase (HRP) and the enzyme Lactate oxidase LOx together with a redox mediator (ferrocene methanol) for the determination of lactate, according to similar strategy described in the literature (Pérez and Fàbregas 2012 Enzyme activity of LOx decays quickly when removed from its natural matrix (Minagawa et al 1998), therefore it is necessary to store it correctly, and for that reason periodical enzymatic activity measurements were carried out. 100 units (U) of LOx were dissolved in 0.1 M PBS and separated in 50 eppendorfs of 20 µL each (2 U) and stored at -20 ºC (Romero et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Hernández-Ibáñez

García‐Cruz

Montiel

et al. 2016

Biosensors and Bioelectronics

135

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Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures, Biosensors and Bioelectronic, http://dx.doi.org/10.1016/j.bios.2015.11.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. This novel electrochemical lactate biosensors analytical efficacy is explored towards the sensing of lactate in model (buffer) solutions and is found to exhibit a linear response towards lactate over the concentration range of 30.4 and 243.9 µM in phosphate buffer solution, with a corresponding limit of detection (based on 3-sigma) of 22.6 µM and exhibits a sensitivity of 3417 ± 131 µA M -1 according to the reproducibility study. These novel electrochemical lactate biosensors exhibit a high reproducibility, with a relative standard deviation of less than 3.8 % and an enzymatic response over 82 % after 5 months stored at 4 ºC. Furthermore, high performance liquid chromatography technique has been utilized to independently validate the electrochemical lactate biosensor for the determination of lactate in a commercial embryonic cell culture medium providing excellent agreement between the two analytical protocols.

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Section: Introductionmentioning

confidence: 99%

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Hernández-Ibáñez

García‐Cruz

Montiel

et al. 2016

Biosensors and Bioelectronics

135

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“…Lactate oxidase (EC 1.13.12.4) belongs to the family of oxidoreductases which catalyses the oxidation of l ‐lactate to pyruvate with the generation of hydrogen peroxide as a side product (Monošík et al . ; Taurino et al . ).…”

Section: Resultsmentioning

confidence: 99%

Rapid lactate oxidase‐based assay for lactate content in milk to ascertain its hygienic quality

Kumar

Sharma

Thakur

et al. 2016

Int J of Dairy Tech

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A rapid method for qualitative and quantitative assaying of L-lactate in milk is described. The method is based on oxidation of lactate by lactate oxidase with the simultaneous formation of H 2 O 2 which subsequently oxidises 2, 4, 6-tribromo 3-hydroxy benzoic acid (TBHBA) in the presence of peroxidase. Oxidised TBHBA then reacts with 4-amino antipyrine to obtain magenta colour (k max = 523 nm). In the qualitative assay, colour change becomes visible in raw milk samples spiked with 300 mg lactate/L. The absorbance increases linearly up to 1000 mg/L. The method can be used for rapid assessment of the hygienic quality of milk.

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“…Так, Monošík et al розробили амперометрич-ний біосенсор з використанням одношарових вуглецевих нанотрубок для вимірювання кон-центрації лактату у вині та харчових продуктах [9]. Gajovic et al розробили біосенсор для ви-значення пірувату у сироватці крові на основі рекомбінантної піруватоксидази [7].…”

Section: вступunclassified

Development of Amperometric Biosensor System for Simultaneous Determination of Pyruvate and Lactate

Topolnikova¹,

Knyzhnykova²,

Кучеренко³

et al. 2017

SEMST

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Анотація. В роботі описано розробку біосенсорної системи, що складається з двох моно-біосенсорів на основі піруватоксидази та лактатоксидази для визначення пірувату та лактату, відповідно. Для іммобілізації ензимів у складі біоселективної мембрани було використано ме-тод захоплення в полімер PVA-SbQ на поверхні амперометричного перетворювача. Як пере-творювачі виступали платинові дискові електроди. В роботі було підібрано єдині умови ви-готовлення та функціонування монобіосенсорів для поєднання їх у біосенсорну систему. Було досліджено оптимальні умови іммобілізації ензимів та параметри буферного розчину для одно-часної роботи біосенсорів, зокрема рН, буферну ємність та іонну силу. Перевірено перехрес-ний вплив субстратів та кофакторів на роботу ензимів. Біосенсорна система характеризувалась гарною операційною стабільністю та відтворюваністю відгуків на піруват та лактат. Отримані аналітичні характеристики біосенсорної системи свідчать про можливість її використання для аналізу лактату та пірувату в реальних біологічних рідинах.Ключові слова: лактат, піруват, біосенсорна система, іммобілізовані ензими, лактатоксида-за, піруватоксидаза

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A rapid method for determination of l-lactic acid in real samples by amperometric biosensor utilizing nanocomposite

Cited by 52 publications

References 33 publications

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Rapid lactate oxidase‐based assay for lactate content in milk to ascertain its hygienic quality

Development of Amperometric Biosensor System for Simultaneous Determination of Pyruvate and Lactate

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