A rapid method for detection and identification of flaviviruses by polymerase chain reaction and nucleic acid hybridization

Puri, Beena; Henchal, Erik A.; Burans, James P.; Porter, Kevin R.; Nelson, William M.; Watts, Douglas M.; Hayes, Curtis G.

doi:10.1007/bf01379104

Cited by 22 publications

(4 citation statements)

References 23 publications

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“…Electrochemical impedance spectroscopy (EIS) is a sensitive and effective technique to probe the interfacial properties of modified electrodes, and was used in this work to compare confirmed dengue fever, DHF and non-infected sera samples. The labelfree biosensor reached a detection limit below 10 fM within 30 min and sensitivity more than 1000 times higher than the reported method using 32 P-labeled probes [62] and more than 10,000 times higher with ethidium bromide staining [63]. As with other lectins, the specificity of concanavalin-A glycoprotein for carbohydrate moieties can be used for developing specific biosensors for sugars.…”

Section: Electrochemicalmentioning

confidence: 79%

Biosensors and rapid diagnostic tests on the frontier between analytical and clinical chemistry for biomolecular diagnosis of dengue disease: A review

Teles

2011

Analytica Chimica Acta

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The past decades have witnessed enormous technological improvements towards the development of simple, cost-effective and accurate rapid diagnostic tests for detection and identification of infectious pathogens. Among them is dengue virus, the etiologic agent of the mosquito-borne dengue disease, one of the most important emerging infectious pathologies of nowadays. Dengue fever may cause potentially deadly hemorrhagic symptoms and is endemic in the tropical and sub-tropical world, being also a serious threat to temperate countries in the developed world. Effective diagnostics for dengue should be able to discriminate among the four antigenically related dengue serotypes and fulfill the requirements for successful decentralized (point-of-care) testing in the harsh environmental conditions found in most tropical regions. The accurate identification of circulating serotypes is crucial for the successful implementation of vector control programs based on reliable epidemiological predictions. This paper briefly summarizes the limitations of the main conventional techniques for biomolecular diagnosis of dengue disease and critically reviews some of the most relevant biosensors and rapid diagnostic tests developed, implemented and reported so far for point-of-care testing of dengue infections. The invaluable contributions of microfluidics and nanotechnology encompass the whole paper, while evaluation concerns of rapid diagnostic tests and foreseen technological improvements in this field are also overviewed for the diagnosis of dengue and other infectious and tropical diseases as well.

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Section: Electrochemicalmentioning

confidence: 79%

Biosensors and rapid diagnostic tests on the frontier between analytical and clinical chemistry for biomolecular diagnosis of dengue disease: A review

Teles

2011

Analytica Chimica Acta

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“…As previously reported, both the sensitivity and specificity of ELISA are more than 95% after the 7th day of disease onset, and specimens collected before 7 days may be negative. 20 Due to a high degree of sensitivity and specificity, the RT-PCR method has a potential application for routine diagnosis of flaviviral infections. More importantly, the samples collected from the areas where JE and dengue are both endemic may show cross-reactivity at the IgM level.…”

Section: Discussionmentioning

confidence: 99%

Viral Etiological Factors Causing Acute Encephalitis Syndrome (AES) In Gaya Division, India

et al. 2019

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Background: From Gaya and adjoining regions, the trend in patients admitted with acute neurological illness was investigated. Illnesses were identified as sudden outbreaks of Japanese virus encephalitis (JE), Herpes simplex virus encephalitis (HSV1&2), and other acute encephalitis syndrome (AES). Objective: In the current study, an investigation was carried out to assess potential infectious pathogens in patients aged 16 years or younger who were admitted to Anugrah Narayan Magadh Memorial Medical College Hospital, Gaya, with encephalitis-like symptoms. Methods: Cross-epidemiological, serological, and molecular biological studies were performed on samples collected from 71 patients below 16 years of age. Patients’ clinical histories, i.e. fever, socio-demographic characteristics, and other clinical data, were extracted from patient files. Results: The results showed confirmed AES cases, including 49.30% JE and 7.04% HSV positive patients. A higher casefatality rate of 40% in JE and 40% HSV cases below 7 years of age were observed during treatment would become an unavoidable concern. The epidemical sex ratio was observed to be higher in girls than in boys (1.26:1). Conclusion: The results suggested that JE virus was found to be a main causative risk factor responsible for disease transmission in the outbreak area.

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“…Because of high degree of sensitivity and specificity, the RT-PCR method has potential application for routine diagnosis of flaviviral infections. 31 The CSF samples of the cases from the areas, where JE and Dengue are both endemic, may show cross reactivity at IgM level in MAC-ELISA so in such cases PCR assay is more useful. Cross-reactions do not occur among different flavivirus with the use of internal primers in the nested PCR.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of antigen detection ELISA and reverse transcriptase PCR in acute stage of Japanese encephalitis prevalent in endemic areas of North-Eastern part of Uttar Pradesh, India

et al. 2015

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A rapid method for detection and identification of flaviviruses by polymerase chain reaction and nucleic acid hybridization

Cited by 22 publications

References 23 publications

Biosensors and rapid diagnostic tests on the frontier between analytical and clinical chemistry for biomolecular diagnosis of dengue disease: A review

Biosensors and rapid diagnostic tests on the frontier between analytical and clinical chemistry for biomolecular diagnosis of dengue disease: A review

Viral Etiological Factors Causing Acute Encephalitis Syndrome (AES) In Gaya Division, India

Comparative evaluation of antigen detection ELISA and reverse transcriptase PCR in acute stage of Japanese encephalitis prevalent in endemic areas of North-Eastern part of Uttar Pradesh, India

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