A Rapid Luteinizing Hormone Radioimmunoassay for the Prediction of Ovulation

Djahanbakhch, O.; McNeilly, Alan S.; Hobson, B. M.; Templeton, Allan

doi:10.1111/j.1471-0528.1981.tb01690.x

Cited by 37 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All women were healthy, aged 32-45 yr, with regular menstrual cycles and had not received any form of hormonal treatment for at least 3 months before taking part in the study. The date of the LH surge was determined by estimation of LH concentrations in serial early morning urine samples collected before operation (19). On this basis, three corpora lutea classified as early luteal (LHϩ1 to LHϩ5), three as midluteal (LHϩ6 to LHϩ10), and three as late luteal (LHϩ11 to LHϩ14) were investigated.…”

Section: Collection Of Corpora Luteamentioning

confidence: 99%

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

Duncan

McNeilly²,

Illingworth

1998

The Journal of Clinical Endocrinology & Metabolism

View full text Add to dashboard Cite

Luteolysis is associated with tissue remodeling probably involving the matrix metalloproteinases (MMPs) and their specific tissue inhibitors (TIMPs). This study investigated the expression and localization of the major MMPs and TIMPs in the human corpus luteum throughout the luteal phase and after luteal rescue with hCG. Corpora lutea (n = 9) were collected at hysterectomy and were dated by serial urinary LH estimation. In addition, corpora lutea (n = 3) were collected from women who had received daily doubling doses of hCG to mimic the hormonal changes of early pregnancy. MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2, and TIMP-3 were investigated by zymography, reverse zymography, Northern blotting, and in situ hybridization. There was no change in the expression of MMP-1, TIMP-1, and TIMP-2 throughout the luteal phase or after luteal rescue. Little TIMP-3 could be detected in the corpus luteum. MMP-9 activity peaked in the early and late luteal phase. The expression and activity of MMP-2 were maximal in the late luteal phase. Exposure to hCG during luteal rescue in vivo was associated with a reduction (P < 0.05) in the expression and activity of MMP-2. Messenger ribonucleic acids (mRNAs) for MMP-1, MMP-2, and TIMP-2 were localized to the connective tissue stroma and the thecal-lutein cells of the corpus luteum. In contrast, TIMP-1 mRNA was localized to the granulosa-lutein cells, and MMP-9 mRNA was expressed in scattered cells within the steroidogenic and nonsteroidogenic cell layers. In conclusion, during maternal recognition of pregnancy, hCG prevents the normal increase in MMP-2 in the late luteal phase. MMPs can function in an environment containing large amounts of TIMP-1, as they have a different cellular localization.

show abstract

Section: Collection Of Corpora Luteamentioning

confidence: 99%

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

Duncan

McNeilly²,

Illingworth

1998

The Journal of Clinical Endocrinology & Metabolism

View full text Add to dashboard Cite

show abstract

“…Plasma concentrations of LH and FSH were measured by radioimmunoassay as described previously (Djahanbakhch et al, 1981;Hunter & Bennie, 1979) while plasma prolactin was measured in a two-site immunoradiometric assay (NETRIA, St Bartholomew's Hospital, London) (Wu et al, 1990). The intra and inter-assay coefficients of variation were < 8 and < 11 Yo respectively for LH, FSH and prolactin.…”

Section: Hormone Assaysmentioning

confidence: 99%

Abnormal twenty‐four hour pattern of pulsatile luteinizing hormone secretion and the response to naloxone in women with hyperprolactinaemic amenorrhoea

Tay

Glasier

Illingworth

et al. 1993

Clinical Endocrinology

View full text Add to dashboard Cite

The suppression of normal ovarian cycles in women with hyperprolactinaemic amenorrhoea is due to a significant reduction in frequency of LH (GnRH) secretion which is not due to an increase in hypothalamic opioid activity. As normal ovarian cycles can occur or be induced by exogenous GnRH in hyperprolactinaemia, it is unlikely that a high level of prolactin by itself inhibits follicular development and ovulation.

show abstract

“…Daily blood samples were collected from all 10 patients for at least 2 days before and 14 days after hysterectomy. In those subjects for whom daily blood samples were available at the appropriate time ovulation was assumed to occur on the day after mid-cycle LH peak (Djahanbakhch, McNeilly, Hobson & Templeton, 1981). In the remainder, in whom only urinary total oestrogen and pregnanediol excretion were available, ovulation was assumed to occur on the day when the urinary excretion of pregnanediol rose at least 2-fold above the level found during the follicular phase.…”

Section: Introductionmentioning

confidence: 99%

Effect of enucleation of the corpus luteum at different stages of the luteal phase of the human menstrual cycle on subsequent follicular development

Baird¹,

Bäckström²,

McNeilly³

et al. 1984

Reproduction

Self Cite

View full text Add to dashboard Cite

Summary. To investigate the mechanism of suppression of follicular development during the luteal phase of the human menstrual cycle, the corpus luteum was enucleated surgically from 10 women at various times after ovulation. In the 24 h after CL enucleation there was an immediate and rapid fall in the concentration of oestradiol and progesterone and a temporary decline in the concentration of FSH and LH. Within 3 days, however, all 10 women showed evidence of renewed follicular activity as indicated by a progressive rise in the concentration of oestradiol. This rise was preceded by a rise in the concentration of FSH and LH, and ovulation, as indicated by a mid-cycle surge in LH and rise in the concentration of plasma progesterone, occurred 16\p=n-\19 days after enucleation. There was no significant difference in the time to ovulation following enucleation at different times of the luteal phase. The post \ x=req-\ operative follicular phase, measured from the time of enucleation, was 3 days longer than that observed pre-operatively from the first day of menstrual bleeding. In the follicular phase of post-operative cycles the concentration of FSH was higher and that of oestradiol lower than the corresponding values before surgery.These results indicate that the absence of healthy antral follicles in the luteal phase of the cycle is due to the inhibitory effects of the corpus luteum. The fact that, after CL enucleation, emergence of the dominant follicle was always preceded by a rise in the concentration of FSH and LH suggests that suppression of gonadotrophins by ovarian steroids secreted by the corpus luteum is responsible for the inhibition of follicular development during the luteal phase of the cycle.

show abstract

A Rapid Luteinizing Hormone Radioimmunoassay for the Prediction of Ovulation

Cited by 37 publications

References 13 publications

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

Abnormal twenty‐four hour pattern of pulsatile luteinizing hormone secretion and the response to naloxone in women with hyperprolactinaemic amenorrhoea

Effect of enucleation of the corpus luteum at different stages of the luteal phase of the human menstrual cycle on subsequent follicular development

Contact Info

Product

Resources

About