A rapid LC-PDA method for the simultaneous quantification of metformin, empagliflozin and linagliptin in pharmaceutical dosage form

Gollu, Gowri; Gummadi, Sowjanya

doi:10.1016/j.pharma.2021.06.003

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Many articles aim to assay both LNG and EMPA using HPLC with a photodiode-array (PDA) detector (9) and reverse-phase RP-HPLC (10). The Chromatographic Analysis HPLC method with UV detection (11), (RP-HPLC method) (12) was demonstrated for the simultaneous analysis of three antidiabetic drugs.…”

Section: Introductionmentioning

confidence: 99%

A specific new analytical method for determining linagliptin in pure and combination drug products (Empagliflozin and Metformin)

Sahloul

Salami

2023

Preprint

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Linagliptin is an oral antidiabetic agent, used to treat type 2 diabetes. It is taken orally independently or in combination with Empagliflozin and Metformin HCl. Chromatographic Analysis methods were demonstrated for simultaneous analysis of two or three antidiabetic drugs (Linagliptin, Empagliflozin and/or Metformin). This article is interested in the validation of a simple, fast and specific method that relies on chemical derivatization and uses a VIS spectrophotometer to determine LINA in pure and combination drug products alongside Empagliflozin and Metformin. The developed method was reached by reacting LNG with Para-Dimethylaminobenzaldehyde (PDAB) 5% in an acidic medium (Hydrochloride 37%) in a water bath to produce a yellow Schiff base which can be measured at a wavelength of 407 nm. The developed method has good accuracy and precision. PDAB does not interact with Empagliflozin or Metformin under these optimal conditions. Therefore, this developed method is distinguished by its accuracy and specificity in assaying Linagliptin both alone and in combination drug products.

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Section: Introductionmentioning

confidence: 99%

A specific new analytical method for determining linagliptin in pure and combination drug products (Empagliflozin and Metformin)

Sahloul

Salami

2023

Preprint

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“…[9][10][11] Literature survey disclosed numerous analytical approaches for the assessment of EPZ, LGT and MTH independently and in combined dosage form by means of UV spectrophotometry, [12][13] HPLC, [14][15][16][17][18][19][20] HPTLC. [21][22][23][24] Moreover, few methods were described for the estimation of EPZ, LGT and MTH in combined formulation/synthetic mixture using HPLC, [25][26][27][28][29] and HPTLC. 2 Nevertheless, the simultaneous estimation of EPZ, LGT and MTH in the ternary mixture has not been reported so far by anyone by UV spectroscopic methods.…”

Section: Introductionmentioning

confidence: 99%

Novel UV Spectroscopic Methods for Simultaneous Assessment of Empagliflozin, Linagliptin and Metformin in Ternary Mixture

Sen¹,

Pandey²,

Maheshwari³

et al. 2022

Ind. J. Pharm. Edu. Res

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Background and Aim: Trijardy XR ® consisting of empagliflozin, linagliptin, and metformin hydrochloride, a fixed-dose combination (tablets) that improves glycemic control in individuals with diabetes mellitus (type 2). The present work presents four spectrophotometric methods that are quick, effortless, accurate and reproducible for the concurrent assessment of the ternary mixture. Materials and Methods: The 1 st approach works on the principle of solving established equations (simultaneous) by measuring absorbance at 224.6, 226 and 237.2 nm for empagliflozin, linagliptin and metformin hydrochloride, respectively. The second method namely ratio difference spectroscopy works by measuring the difference in amplitude at two different wavelengths in the ratio spectra. Whereas, the derivative ratio spectrum zero-crossing approach (third approach) relied on the utilization of the derivative ratio signals at zero-crossing locations. The fourth approach is the double divisor-ratio spectra derivative approach in which the first derivative of ratio spectrum was acquired and the concentrations of all 3 drugs in their combination were quantified. Results and Discussion: All the three drugs exhibited excellent linear correlation in the concentration series of 2-10 µg/ml for simultaneous equation method and 0.5-10 µg/ml for all the other methods with an exceptional correlation coefficient value. Furthermore, the projected approaches were validated as per ICH strategies and which displayed good precision, accuracy and sensitivity. Conclusion:The developed spectrophotometric approaches when compared to other analytical procedures are regarded to be more cost-effective because they do not require expensive solvents or sophisticated instruments. Therefore, the projected methods could be effectively employed for the concurrent assessment of empagliflozin, linagliptin and metformin hydrochloride in ternary mixture.

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Empagliflozin containing chitosan-alginate nanoparticles in orodispersible film: preparation, characterization, pharmacokinetic evaluation and itsin-vitroanticancer activity

Suhani

Sonali

Garg

et al. 2022

Drug Development and Industrial Pharmacy

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A rapid LC-PDA method for the simultaneous quantification of metformin, empagliflozin and linagliptin in pharmaceutical dosage form

Cited by 4 publications

References 10 publications

A specific new analytical method for determining linagliptin in pure and combination drug products (Empagliflozin and Metformin)

A specific new analytical method for determining linagliptin in pure and combination drug products (Empagliflozin and Metformin)

Novel UV Spectroscopic Methods for Simultaneous Assessment of Empagliflozin, Linagliptin and Metformin in Ternary Mixture

Empagliflozin containing chitosan-alginate nanoparticles in orodispersible film: preparation, characterization, pharmacokinetic evaluation and itsin-vitroanticancer activity

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