A rapid lateral flow immunoassay for serological diagnosis of pertussis

Multiplex Point-of-Care Tests for the Determination of Antibodies after Acellular Pertussis Vaccination

“…In conclusion, this exploratory study indicates that the developed multiplex LFIA can be a suitable option for the point-of-care measurement of antibodies against pertussis. Compared to ELISAs and other multiplex formats, the LFIA offers a very rapid readout, a low amount of antigens and samples, and ease of use [20]. The specificity of the spatially multiplexing design gives an encouraging glance for future work especially with antigens, which are not included in the current acellular vaccines.…”

“…Label conjugates: A total of 375 µg of Protein A (#RL240425, Thermo Fisher Scientific, Meridian Rd., Rockford, IL, USA) was conjugated with activated fluoro-Max carboxyl modified microparticles containing europium chelates (diameter 99 nm, Thermo Fisher Scientific), as described earlier [20]. Protein A has the ability to bind to anti-human IgA, IgG, and IgM simultaneously, and has been preferred in our assays due to its small size in comparison to larger secondary antibody conjugates [22,23].…”

“…Lateral flow test strips and multiplex assay: The preparation of the strips and the test procedure was performed following a similar method to Salminen et al [20] with the following modifications: The captured reagents on the test strips were purified PRN, PT and FHA-kindly provided by GlaxoSmithKline (Belgium), and rabbit anti-goat IgG antibody on the control line ( Figure 1). Lateral flow test strips were assembled on a plastic support (G&L Precision Die Cutting, San Jose, CA, USA) with a cellulose absorbent pad (CFSP223000, Millipore, Bedford, MA, USA) and a glass fiber sample pad (G041, Millipore).…”

“…Cost-efficient and high-throughput multiplex serological assays in the pertussis field already have a broad application [16][17][18][19]. We have reported earlier on a simple, quantitative and rapid lateral flow (LF)-based platform for anti-PT IgG serological diagnostics of pertussis without the complexity of common laboratory practicalities [20]. The apparent advantages of multiplexing a serological response with LF immunoassays (LFIA) are related to the easily accomplished test setting through multiple test lines and a common label without any further complications either to design or to perform the test in comparison to single test line-based tests.…”

The use of lateral flow immunoassays for the detection of fentanyl in seized drug samples and postmortem urine

“…A promising solution for rapid fentanyl detection is the use of paper-based lateral flow immunoassays (LFIs). Use of LFIs for the simple, rapid detection of various biological and chemical compounds has gained popularity in a variety of disciplines including, but not limited to, point-of-care medicine (e.g., pertussis, streptococcus) [9,10], agricultural surveillance (e.g., red fire ant, Grapevine Leafroll-Associated Virus) [11,12], biodefense (e.g., Bacillus anthracis, Yersinia pestis) [13,14], and more recently, forensic science (e.g., illegal drugs) [15,16]. These assays employ antibodies directed against the target (i.e., fentanyl), which are incorporated into the surface of the assay.…”