A Rapid Laboratory Method of Assessing Susceptibility of <i>Hevea</i> Clones to <i>Oidium Heveae</i>

Lim, T. M.

doi:10.1017/s0014479700005809

Cited by 6 publications

(3 citation statements)

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“…This is the first report of significant marker-trait associations for resistance against foliar diseases caused by Phytophthora spp., Corynespora cassiicola, and Colletotrichum acutatum in rubber. Similar to what has been reported for resistance to several other fungal pathogens of rubber [28][29][30], continuous variation was observed, and multiple loci of small effect were detected. Among the mapping progeny, four clones carried favorable alleles at all 12 resistance-associated SNPs.…”

Section: Discussionsupporting

confidence: 86%

Analysis of Genetic Diversity and Resistance to Foliar Pathogens Based on Genotyping-by-Sequencing of a Para Rubber Diversity Panel and Progeny of an Interspecific Cross

Roy

Goonetilleke

Joseph

et al. 2022

Plants

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Para rubber trees (Hevea brasiliensis) are the largest major source of natural rubber in the world. Its major pathogens are Phytophthora spp., Corynespora cassiicola, and Colletotrichum spp. A rubber diversity panel of 116 clones using over 12,000 single nucleotide polymorphisms (SNPs) from DArTSeq genotyping revealed clear phylogenetic differences in clones that originated from different geographical regions of the world. An integrated linkage map constructed with an F1 progeny of 86 from an interspecific cross between H. brasiliensis and H. benthamiana using 23,978 markers [10,323 SNPs and 13,655 SilicoDArTs] spanned 3947.83 cm with 0.83 cm average marker-interval. The genome scaffolds that were anchored to the linkage map, covering 1.44 Gb of H. brasiliensis reference genome, revealed a high level of collinearity between the genetic map and reference genome. Association analysis identified 12 SNPs significantly associated with the resistance against Phytophthora, Corynespora, and Colletotrichum in six linkage groups: 2, 6, 12, 14, 17, and 18. Kompetitive Allele-Specific PCR marker assays were developed for those 12 SNPs, screened with 178 individuals, and detected clear separation between two genotypes. Within the proximity to those SNPs, 41 potentially key genes that have previously been reported to associate with plant disease resistance were predicted with high confidence.

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Section: Discussionsupporting

confidence: 86%

Analysis of Genetic Diversity and Resistance to Foliar Pathogens Based on Genotyping-by-Sequencing of a Para Rubber Diversity Panel and Progeny of an Interspecific Cross

Roy

Goonetilleke

Joseph

et al. 2022

Plants

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“…Each petri dish setup included one lentil leaf, and leaflets were oriented abaxial surface facing upward. Abaxial surfaces of the leaves were inoculated using a fine paint brush covered with fresh conidia from greenhouse-grown lentil plants until the surfaces were perceptibly tinged with white color (20). The paint brush was disinfected by rinsing in 95% ethanol followed by air-drying between treatments.…”

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confidence: 99%

Erysiphe trifolii Causing Powdery Mildew of Lentil (Lens culinaris)

et al. 2009

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The taxonomy of the powdery mildew fungus infecting lentil in the Pacific Northwest (PNW) of the United States was investigated on the basis of morphology and rDNA internal transcribed spacer (ITS) sequences. Anamorphic characters were in close agreement with descriptions of Erysiphe trifolii. However, teleomorphs formed chasmothecial appendages with highly branched apices, whereas E. trifolii has been described as producing flexuous or sometimes loosely branched appendages. Branched appendages have been described in Erysiphe diffusa, a fungus reported from species of Lens, Glycine, and Sophora, raising the possibility that the PNW fungus could be E. diffusa. Examination of morphological characters of an authentic specimen of E. trifolii from Austria determined that it included chasmothecial appendages resembling those seen in PNW specimens. Furthermore, ITS sequences from five powdery mildew samples collected from lentils in PNW greenhouses and fields from 2006 to 2008 were identical to one another, and exhibited higher similarity to sequences of E. trifolii (99%) than to those of any other Erysiphe spp. available in GenBank. Parsimony analysis grouped the lentil powdery mildew into a clade with Erysiphe baeumleri, E. trifolii, and E. trifolii–like Oidium sp., but indicated a more distant relationship to E. diffusa. In greenhouse inoculation studies, the lentil powdery mildew fungus did not infect soybean genotypes known to be susceptible to E. diffusa. The pathogenicity of E. trifolii on lentil was confirmed using modified Koch's postulates. This is the first report of E. trifolii infecting lentil. E. diffusa and E. trifolii have different host ranges, so the discovery of E. trifolii on lentil has implications both for determining species of powdery mildews on cool-season grain legumes, and in disease management.

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“…Conidia from P. sativum were used to inoculate detached leaves of M. albus and Lens culinaris , and conidia from M. albus and Lens culinaris were used separately to inoculate detached leaves of P. sativum . Conidia were applied on the abaxial leaf surfaces using a fine paint brush (Lim, 1973) until a white powdery appearance was visible. The paint brush was disinfested by rinsing in 95% ethanol, followed by air‐drying, between each treatment.…”

Section: Methodsmentioning

confidence: 99%

Erysiphe trifolii– a newly recognized powdery mildew pathogen of pea

et al. 2010

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Diversity of powdery mildew pathogens infecting pea (Pisum sativum) in the US Pacific Northwest was investigated using both molecular and morphological techniques. Phylogenetic analyses based on rDNA ITS sequences, in combination with assessment of morphological characters, defined two groups of powdery mildews infecting pea. Group I (five field samples and three glasshouse samples) had ITS sequences 99% similar to those of Erysiphe pisi in GenBank and exhibited simple, mycelioid type of chasmothecial appendages typical of E. pisi. Erysiphe pisi is normally considered as the powdery mildew pathogen of pea. Group II (four glasshouse samples and two field samples) had ITS sequences 99% similar to those of E. trifolii and produced chasmothecia with dichotomously branched appendages similar to those of E. trifolii. There are fourteen nucleotide differences in the ITS region between the two groups. The correlation of rDNA ITS sequences with teleomorphic features for each of the two groups confirms their identity. Repeated samplings and artificial inoculations indicate that both E. pisi and E. trifolii infect pea in the US Pacific Northwest. Erysiphe trifolii is not previously known as a pathogen of pea. The existence of two distinct powdery mildew species infecting pea in both glasshouse and field environments may interfere with the powdery mildew-resistance breeding programmes, and possibly explains putative instances of breakdown of resistance in previously resistant pea breeding lines.

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A Rapid Laboratory Method of Assessing Susceptibility of Hevea Clones to Oidium Heveae

Abstract: SUMMARYA detached-leaf technique is described for assessing susceptibility of Hevea clones to Oidium heveae, based on intensity of sporulation. This offers a rapid, systematic method of screening clones in vitro against the disease, in place of the current nursery and field methods.

Cited by 6 publications

References 0 publications

Analysis of Genetic Diversity and Resistance to Foliar Pathogens Based on Genotyping-by-Sequencing of a Para Rubber Diversity Panel and Progeny of an Interspecific Cross

Analysis of Genetic Diversity and Resistance to Foliar Pathogens Based on Genotyping-by-Sequencing of a Para Rubber Diversity Panel and Progeny of an Interspecific Cross

Erysiphe trifolii Causing Powdery Mildew of Lentil (Lens culinaris)

Erysiphe trifolii– a newly recognized powdery mildew pathogen of pea

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