A rapid isolation method of extracellular proteins produced by Pseudomonad strains

Abstract

Manufacturing of protein/enzymes by biotechnological methods may contribute to develop new protein/enzyme preparations useful in various sectors of industry. Microbial enzymes released in growing medium and difficult to isolate. In this study, a rapid method has been developed for isolating extracellular proteins from cultured nutrient broth. After removing the bacterial cells from cultured broth through 0.4mm filter paper and dissolved proteins recovered by precipitation with 5% trichloroacetic acid (TCA). Sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the isolated crude enzymes from bacterial cell free culture filtrate showed the presence of different bands in the electrophoretic field.