A Rapid Hæmagglutination Test for Hepatitis-B Antigen

Cayzer, I; Dane, D. S.; Cameron, C.H.; Denning, J.V.

doi:10.1016/s0140-6736(74)91259-8

Cited by 75 publications

(24 citation statements)

References 5 publications

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“…All donors' sera for 2.5 years were screened at a dilution of 1 in 8 for HBsAg by the original Hepatest [2] (Wellcome Reagents Ltd, Beckenham, Kent), and all positive sera were titrated against the Hepatest test cells. The specificity of low titre sera was confirmed by neutralisation and absorp tion.…”

Section: Introduction Methodsmentioning

confidence: 99%

Lognormal Distribution of HBsAg Strengths in an English Population

Renton¹,

Taberner²,

Roach³

1979

Vox Sanguinis

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The frequency distribution of reverse passive haemagglutination titres for HBsAg in the sera of 78 positive blood donors found during a 2.5-year period closely follows a lognormal distribution curve. This lognormal distribution suggests that the total population of HBsAg-positive donors during this period was approximately 84. The modified Hepatest now in use will be expected to detect 82 of these, and to miss only 1 HBsAg-positive donor in 50,000 screening tests of Manchester blood donors.

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Section: Introduction Methodsmentioning

confidence: 99%

Lognormal Distribution of HBsAg Strengths in an English Population

Renton¹,

Taberner²,

Roach³

1979

Vox Sanguinis

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“…Serum specimens were tested for HBsAg either (until 1974) by immunoelectroosmophoresis (IEOP) (White et al 1971) or subsequently by reversed passive haemagglutination (RPHA) (Cayzer et al 1974) using the Hepatest kit (Wellcome Reagents Ltd). RPHA titres were determined by testing doubling dilutions from 1/8 to 1/128000.…”

Section: Methodsmentioning

confidence: 99%

e-Antigen: a link between immune response and infectivity in hepatitis B?

Turner

Green

Blundell

1978

J. Hyg.

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SUTMMARYIn a study of the distribution of e-antigen and anti-e in subjects whose blood was positive for hepatitis B surface antigen (HBsAg), patients with acute hepatitis B who were tested during the incubation period were all e-antigen-positive but after the onset of illness e-antigen was detected in only 11%. Persistence, and in some instances reappearance of e-antigen in those who became long-term carriers of HBsAg was associated with high titres of HBsAg. There was a high incidence of e-antigen in those conditions in which cell-mediated immunity may be depressed, including Down's syndrome and chronic renal failure.The majority of HBsAg carriers identified as sources of infection were e-antigenpositive. A positive reaction for e-antigen is evidently associated with a defective immune response to hepatitis B virus infection which permits continued replication of virus in liver cells accompanied by high titres of HBsAg, numerous Dane particles and detectable DNA polymerase in the blood with consequently a greater likelihood of transmitting infection.Although it cannot be assumed that anti-e positive carriers of HBsAg are not infective, it may be necessary, in the assessment of passive or active immunization for the control of hepatitis B, to take into account the e-antigen/antibody status of possible sources of infection.

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“…Hepatest (Wellcome Reagents Ltd) uses turkey erythrocytes coated with purified horse antibody to HBsAg [1]. The test was carried out according to the manufac turer's instructions, adding 25 [A of erythrocyte suspension to 25 [A 1:8 dilution of the test sample.…”

Section: Methodsmentioning

confidence: 99%

Detection of Hepatitis-B Surface Antigen in Plasma Samples by Reversed Passive Haemagglutination Tests

Combridge¹,

Kennedy²

1976

Vox Sanguinis

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A comparison of two commercial reversed passive haemagglutination tests with a counter-immunoelectrophoretic test, used to detect hepatitis-B surface antigen in reconstituted freeze-dried plasma, showed an increase in the number of positive results by the haemagglutination tests. No difficulty was encountered with the appearance of non-specific agglutination provided the plasma was heated at 60°C for 30 min, frozen overnight at -35°C, thawed and centrifuged before testing.

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A Rapid Hæmagglutination Test for Hepatitis-B Antigen

Cited by 75 publications

References 5 publications

Lognormal Distribution of HBsAg Strengths in an English Population

Lognormal Distribution of HBsAg Strengths in an English Population

e-Antigen: a link between immune response and infectivity in hepatitis B?

Detection of Hepatitis-B Surface Antigen in Plasma Samples by Reversed Passive Haemagglutination Tests

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