A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein

Chen, Xuan; Liu, Xianglian; Zhang, Chuanyu; Meng, Hao; Liu, Biwu; Wei, Xueyong

doi:10.1016/j.talanta.2022.123661

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…Compared to the traditional sandwich method, this SA–Bio sandwich FLISA method further improves the sensitivity (∼4.25 fold) and expands the detection range, which indicates the introduction of the SA-Bio system to realize the signal amplification of sandwich FLISA. However, the LOD in this SA–Bio FLISA for CRP detection is not comparable to that of the Cd-based QD and novel Au-based FLISA. − For low-toxicity In-based QD labels, , this SA–Bio FLISA reveals the lowest LOD (Table S5).…”

Section: Resultsmentioning

confidence: 87%

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Zhang,

Xu,

Zhang

et al. 2024

Inorg. Chem.

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Nontoxic, highly sensitive InP quantum dot (QD) fluorescent immunoassay probes are promising biomedical detection modalities due to their unique properties. However, InP-based QDs are prone to surface oxidation, and the stability of InP QD-based probes in biocompatible environments remains a crucial challenge. Although the thick shell can provide some protection during the phase transfer process of hydrophobic QDs, the photoluminescence quantum yield (PLQY) is generally decreased because of the contradiction between lattice stress relaxation and thick shell growth. Herein, we developed thick-shell InP-based core/shell QDs by inserting a ZnSeS alloy layer. The ternary ZnSeS intermediate shell could effectively facilitate lattice stress relaxation and passivate the defect states. The synthesized InP/ZnSe/ZnSeS/ZnS core/alloy shell/shell QDs (CAS-InP QDs) with nanostructure tailoring revealed a larger size, high PLQY (90%), and high optical stability. After amphiphilic polymer encapsulation, the aqueous CAS-InP QDs presented almost constant fluorescence attenuation and stable PL intensity under different temperatures, UV radiation, and pH solutions. The CAS-InP QDs were excellent labels of the fluorescence-linked immunosorbent assay (FLISA) for detecting C-reactive protein (CRP). The biotin–streptavidin (Bio–SA) system was first introduced in the FLISA to further improve the sensitivity, and the CAS-InP QDs-based SA–Bio sandwich FLISA realized the detection of CRP with an impressive limit of detection (LOD) of 0.83 ng/mL. It is believed that the stable and sensitive InP QD fluorescent probes will drive the rapid development of future eco-friendly, cost-effective, and sensitive in vitro diagnostic kits.

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Section: Resultsmentioning

confidence: 87%

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Zhang,

Xu,

Zhang

et al. 2024

Inorg. Chem.

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“…Chen et al. argue that any detection time under 40 minutes qualifies as rapid detection, [39] and there are numerous fluorescence sensors with detection times of not less than 40 min were reported. For instance, Vaishanav et al.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, though the reaction time of 40 min was longer in this work, it is considered acceptable. Chen et al argue that any detection time under 40 minutes qualifies as rapid detection, [39] and there are numerous fluorescence sensors with detection times of not less than 40 min were reported. For instance, Vaishanav et al utilized Mn 2 + -doped CdTe/ZnS QDs to develop a folic acid sensor with a reaction time of 40 min; [40] Peng et al reported an aptamer-based fluorescent biosensor for ATP detection based on the CRISPR-Cas12a system, and the reaction time was also 40 min; [41] A fluorescent progesterone biosensor was successfully established with a reaction time of 2.5 hours; [42] A ratiometric fluorescent sensor with dual target recognition sites was designed for the sensitive detection of Burkholderia pseudomallei DNA, and the detection time was 40 min; [43] Li et al developed a method for the rapid detection of ochratoxin A based on aptamer-regulated DNA-silver nanoclusters with a reaction time of 45 min; [44] Zhang et al reported A ratiometric fluorescence sensor for determination of choline based on gold nanoclusters and enzymatic reaction with the reaction time of 50 min, [45] and so on.…”

Section: Establishment Of Fa Sensormentioning

confidence: 99%

A Highly Sensitive and Selective Fluorescent Sensor for Folic Acid Detection Based on D‐penicillamine Stabilized Ag/Cu Alloy Nanoclusters

Mei Zhang,

Xue Dong,

Li Wu

et al. 2024

ChemBioChem

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In this work, a highly sensitive and selective method for detecting folic acid (FA) was developed using D‐penicillamine (DPA) stabilized Ag/Cu alloy nanoclusters (DPA@Ag/Cu NCs). The yellow emission of DPA@Ag/Cu NCs was found to be quenched upon the addition of FA to the system. The fluorescence intensity quenching value demonstrated a linear relationship with FA concentrations ranging from 0.01 to 1200 μM, with a limit of detection (LOD) of 5.3 nM. Furthermore, the detection mechanism was investigated through various characterization analyses, including high resolution transmission electron microscopy, fluorescence spectra, ultraviolet‐visible absorption spectra, and fluorescence lifetime. The results indicated that the fluorescence quenching induced by FA was a result of electron transfer from FA to the ligands of DPA@Ag/Cu NCs. The selectivity of the FA sensor was also evaluated, showing that common amino acids and inorganic ions had minimal impact on the detection of FA. Moreover, the standard addition method was successfully applied to detect FA in human serum, chewable tablets and FA tablets with promising results. The use of DPA@Ag/Cu NCs demonstrates significant potential for detecting FA in complex biological samples.

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“…The most commonly used techniques for CRP detection in clinical laboratories include enzyme-linked immunosorbent assays (ELISAs) [ 6 , 7 , 8 ], immunoturbidimetric assays [ 9 , 10 , 11 ], latex immunoagglutination assays [ 12 , 13 ], fluorescence assays [ 14 , 15 , 16 ], chemiluminescence assays [ 17 , 18 ], surface plasmon resonance-based assays [ 19 ], and electrochemical assays [ 20 , 21 , 22 , 23 , 24 , 25 ]. However, these methods are time-consuming and require skilled operators.…”

Section: Introductionmentioning

confidence: 99%

Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein

Chen,

Lu,

Tseng

et al. 2024

Biosensors

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A microfluidic immuno-biosensor detection system consisting of a microfluidic spectrum chip and a micro-spectrometer detection device is presented for the rapid point-of-care (POC) detection and quantification of high-sensitivity C-reactive protein (hs-CRP) in urine. The detection process utilizes a highly specific enzyme-linked immunosorbent assay (ELISA) method, in which capture antibodies and detection antibodies are pre-deposited on the substrate of the microchip and used to form an immune complex with the target antigen. Horseradish peroxidase (HRP) is added as a marker enzyme, followed by a colorimetric reaction using 3,3′,5,5′-tetramethylbenzidine (TMB). The absorbance values (a.u.) of the colorimetric reaction compounds are measured using a micro-spectrometer device and used to measure the corresponding hs-CRP concentration according to the pre-established calibration curve. It is shown that the hs-CRP concentration can be determined within 50 min. In addition, the system achieves recovery rates of 93.8–106.2% in blind water samples and 94.5–104.6% in artificial urine. The results showed that the CRP detection results of 41 urine samples from patients with chronic kidney disease (CKD) were highly consistent with the conventional homogeneous particle-enhanced turbidimetric immunoassay (PETIA) method’s detection results (R2 = 0.9910). The experimental results showed its applicability in the detection of CRP in both urine and serum. Overall, the results indicate that the current microfluidic ELISA detection system provides an accurate and reliable method for monitoring the hs-CRP concentration in point-of-care applications.

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A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein

Cited by 12 publications

References 22 publications

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

A Highly Sensitive and Selective Fluorescent Sensor for Folic Acid Detection Based on D‐penicillamine Stabilized Ag/Cu Alloy Nanoclusters

Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein

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