A Rapid, Fluorescence Switch-On Biosensor for Early Diagnosis of Sorghum Mosaic Virus

Abstract

For the first time, a nanobiosensor was established for Sorghum mosaic virus (SrMV) detection. The biosensor consists of cadmium telluride quantum dots (CdTe QDs) conjugated to the specific antibody (Ab) against SrMV coat protein (CP) and carbon quantum dots (C QDs) labeled with SrMV coat protein. The formation of the fluorophore-quencher immunocomplex CdTe QDs-Ab+C QDs-CP led to a distinct decrease in the fluorescence intensity of CdTe QDs. Conversely, the emission intensity of CdTe QDs recovered upon the introduction of unlabeled CP. The developed biosensor showed a limit of detection of 44 nM in a linear range of 0.10–0.54 μM and exhibited the strongest fluorescence intensity (about 47,000 a.u.) at 552 nm. This strategy was applied to detect purified CP in plant sap successfully with a recovery rate between 93–103%. Moreover, the feasibility of the proposed method was further verified by the detection of field samples, and the results were consistent with an enzyme-linked immunosorbent assay (ELISA). Contrarily to ELISA, the proposed biosensor did not require excessive washing and incubation steps, thus the detection could be rapidly accomplished in a few minutes. The high sensitivity and short assay time of this designed biosensor demonstrated its potential application in situ and rapid detection. In addition, the fluorescence quenching of CdTe QDs was attributed to dynamic quenching according to the Stern-Volmer equation.

Recent advances in biosensors for screening plant pathogens

“…2.5 copies of MCMV coat protein genes Leaves 0.96 pg of the total RNA Dual-uorescent signal 68 Tobacco mosaic virus 0.08 fM for FAM and 0.07 fM for TAMRA Leaves Fluorescent 69 Sorghum mosaic virus (SrMV) 44 nM Leaves from sugarcane plants Colorimetric 73 Apple necrotic mosaic virus (ApNMV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple scar skin viroid (ASSVd) 250 viral copies per reaction for ASPV and ASGV; 2500 copies for the ApNMV, ACLSV, and ASSVd…”

“…In light of this, Han et al fabricated a fluorescence switch-on biosensor for sorghum mosaic virus detection leveraging cadmium telluride quantum dots (CdTe QDs) as a fluorescent tag. 69 The CdTe QDs were conjugated to specific antibody (Ab) against the coat protein of the sorghum mosaic virus to enhance the specificity of sorghum mosaic virus detection. The CdTe QD-based biosensors possessed a LOD of 44 nM in a linear range of 0.10–0.54 μM and showed the strongest fluorescence intensity (roughly 47 000 a.u.)…”

Breaking boundaries in microbiology: customizable nanoparticles transforming microbial detection

“…The limit of the detection is found to be 2.2 µg mL −1 . 56 The use of a bioactive hybrid metal-organic framework (MOF) nanobased biosensor successfully detected the presence of the SARS-CoV-2 spike antigen. The metal-organic framework is prepared by a facile and high gravity technique followed by conjugation with the SARS-CoV-2 spike antigen.…”

Optimization and Validation of Reverse Transcription Recombinase-Aided Amplification (RT-RAA) for Sorghum Mosaic Virus Detection in Sugarcane

“…However, these methods have limitations too, and the detection cycle might take up to an hour since they require sophisticated laboratory equipment and costly temperature-controlled amplification devices. In addition, a more advanced nanobiosensor has also been established for the detection of SrMV, which can be completed in a few minutes but still requires a relevant fluorescence observation device [ 14 ].…”