A Rapid Determination Method for Anthocyanin Profiling in Grape Genetic Resources

Shiraishi, Masayuki; Yamada, Masahiko; Mitani, Nobuhito; Ueno, Takatoshi

doi:10.2503/jjshs.76.28

Cited by 34 publications

(18 citation statements)

References 13 publications

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“…The total anthocyanin content in grape skin was analyzed by the method of Shiraishi et al (2007), and was expressed as mg of cyanidin-3-monoglucoside (Extrasynthèse, Genay, France) equivalent per g of fresh berry skin. Total flavonol contents in grape skin Hort.…”

Section: Analysis Of Anthocyanin and Flavonol Contentsmentioning

confidence: 99%

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

et al. 2015

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Flavonoid biosynthesis in grape (Vitis spp.) berry skin is affected by environmental factors such as light and temperature. However, the components of the light signaling and low-temperature-induced abscisic acid (ABA) signaling networks related to flavonoid accumulation in grape berry skin have not been fully elucidated, and how environmental conditions affect these components remains unclear. To clarify the details of the possible light-and ABA-related signal transduction networks, we developed a grape oligo-DNA microarray (38,549 independent probes) using the publicly available genomic sequence of grape, and performed comprehensive transcriptome analysis using detached 'Pione' grape (Vitis labruscana × V. vinifera) berries cultured under different light and temperature conditions. Using the microarray data, we explored the light-inducible and low-temperature-inducible genes in post-veraison grape berries. We identified 40 lightinducible genes, 55 low-temperature-inducible genes, and 34 genes induced by light plus low temperature. Among these, we selected elongated hypocotyl 5 (HY5), open stomata 1 (OST1), and enhanced response to ABA 1 (ERA1) as candidate light-inducible, low-temperature-inducible, and light-plus low-temperature-inducible genes, respectively. We investigated their detailed expression characteristics in grape accessions by means of quantitative real-time polymerase chain reaction analyses, and hypothesized that HY5, OST1, and ERA1 might be involved in flavonoid biosynthesis via light signaling and low-temperature signaling networks. We also established an extensive catalog of gene expression patterns to support future investigations of other candidate genes that respond to light and temperature in grape berry skin.

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Section: Analysis Of Anthocyanin and Flavonol Contentsmentioning

confidence: 99%

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

et al. 2015

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“…5). Because the relationship between skin color and anthocyanin content varies among cultivars (Shiraishi et al, 2007;Watanabe and Shiraishi, 1991), we calculated the linear regression separately for each cultivar. The anthocyanin contents in the berry skins at harvest could be predicted by using a combination of the regressions shown in Figure 5 and the regressions to predict the skin color rating (Table 4).…”

Section: Prediction Of the Anthocyanin Content In Berry Skinsmentioning

confidence: 99%

“…A few clusters harvested in each vineyard in 2011 were used to measure the anthocyanin content of the berry skins by the method of Shiraishi et al (2007) with a slight modification. After rating the skin color as above, about 0.1 g of skin near the equator of each of 10 berries from the same cluster was removed, combined, blotted dry on paper towels (Crecia, Japan), macerated in 10 mL of 50% v/v aqueous acetic acid for 12 h at 4°C in the dark, and the suspension was filtered (No.…”

Section: Anthocyanin Content In the Berry Skinmentioning

confidence: 99%

Prediction of Skin Coloration of Grape Berries from Air Temperature

et al. 2018

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To develop a method for predicting the skin color of grape berries of three cultivars of Vitis labrusca L. × Vitis vinifera L. grown in Japan, we investigated the relationship between skin color and air temperature in the grape production areas of 18 prefectures. When mean air temperature during the 40 days before harvest date was ≥24°C, the skin color ratings of 'Kyoho', 'Pione', and 'Suzuka' were significantly negatively correlated with temperature. Skin color ratings decreased by about 1 unit per 1°C increase; at a given mean air temperature during this period, the rating of 'Suzuka' was higher (by 0.7 units) than that of 'Kyoho', which was higher (by 1.0 unit) than that of 'Pione'. Because an approach to predict harvest date has not been established, we developed a method to predict skin color at harvest based on air temperature after the fullflowering date. We found the times that had a strong negative correlation between the mean air temperature and the skin color rating at harvest was 43 days from 50 DAF (days after full flowering) for 'Kyoho', 46 days from 46 DAF for 'Pione', and 42 days from 52 DAF for 'Suzuka'. We obtained a linear regression equation for the relationship between the skin color rating at harvest and the mean air temperature during the periods. If the full-flowering date is known, it is possible to predict skin color at harvest by using this equation and the predicted air temperature after full flowering. We also developed a method for predicting anthocyanin contents in berry skins at harvest using significant regressions among the skin color rating, the skin anthocyanin content, and mean air temperature.

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“…Anthocyanins were extracted and analyzed according to a slight modification of Shiraishi et al's method [19]. One gram of skin powder prepared in liquid nitrogen with a mortar and pestle was homogenized in 40 mL of 50% (v/v) aqueous acetic acid with a mortar and pestle.…”

Section: Extraction Of Anthocyaninsmentioning

confidence: 99%

Flavonoid Profiles of Wild Grapes Native to Japan: Vitis coignetiae Pulliat and Vitis ficifolia Bunge var. ganebu Hatusima

Shiozaki¹,

Murakami²

2017

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Flavonoids are a group of natural compounds in plants with versatile health benefits for humans. Grapes are a dietary source of flavonoids and the flavonoid components in grape berries can depend on the grape species and cultivar. In this experiment, proanthocyanidins, flavonols, and anthocyanins were analyzed in Vitis coignetiae and V. ficifolia var. ganebu, wild grapes native to Japan, and compared with those in V. labruscana cv. Muscat Bailey A, to evaluate the potential of the wild grapes as a grape resource. Proanthocyanidin contents in seeds were lower in the two wild grapes than in Muscat Bailey A.However, the skin of V. ficifolia var. ganebu was the richest source of proanthocyanidins. Flavonol levels in the skins of the two wild grapes were lower than that in the skin of Muscat Bailey A. Colorimetry determined that the total anthocyanin content in the skin of V. ficifolia var. ganebu was 6 times and 7 times higher, respectively, than those of V. coignetiae and Muscat Bailey A.Although monoglucoside anthocyanin levels analyzed by high-pressure liquid chromatography (HPLC) were in the order Muscat Bailey A > V. ficifolia var. ganebu > V. coignetiae, most of the diglucoside and acylated monoglucoside and diglucoside anthocyanin levels identified by HPLC-mass spectrometry were highest in V. ficifolia var. ganebu. These data suggest that V. ficifolia var. ganebu might be a novel source of flavonoids and superior to V. coignetiae as a source of flavonoids.

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A Rapid Determination Method for Anthocyanin Profiling in Grape Genetic Resources

Cited by 34 publications

References 13 publications

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

Prediction of Skin Coloration of Grape Berries from Air Temperature

Flavonoid Profiles of Wild Grapes Native to Japan: <i>Vitis coignetiae </i>Pulliat and<i> Vitis ficifolia </i>Bunge var. <i>ganebu</i> Hatusima

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A Rapid Determination Method for Anthocyanin Profiling in Grape Genetic Resources

Cited by 34 publications

References 13 publications

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

Prediction of Skin Coloration of Grape Berries from Air Temperature

Flavonoid Profiles of Wild Grapes Native to Japan: &lt;i&gt;Vitis coignetiae &lt;/i&gt;Pulliat and&lt;i&gt; Vitis ficifolia &lt;/i&gt;Bunge var. &lt;i&gt;ganebu&lt;/i&gt; Hatusima

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Flavonoid Profiles of Wild Grapes Native to Japan: <i>Vitis coignetiae </i>Pulliat and<i> Vitis ficifolia </i>Bunge var. <i>ganebu</i> Hatusima