“…The effects of modified heating conditions and acid concentration on the assay of estrone, estradiol, and estriol were investigated (97), and the excitation and emission spectra of these and other estrogens were recorded (581). Simplified fluorometric methods were studied and improvements suggested (127, 156). Automated procedures were described for the assay of urinary estrogens in nonpregnancy (646), pregnancy (444), and late pregnancy (709), and for ethinylestradiol in pharmaceutical preparations (336).…”