A rapid assay for evaluation of antiviral activity against coxsackie virus B3, influenza virus A, and herpes simplex virus type 1

In Vivo and in Vitro Antiviral Activities of Calycosin-7-O-.BETA.-D-glucopyranoside against Coxsackie Virus B3

“…Infected cells receiving no drugs served as the virus control, and infected cells treated by ribavirin were included as the positive control. When 100% CPE was observed at 3-4 d post-infection, 12) the drug concentration that achieved 50% inhibition of the observed CPE was determined as IC 50 , which was calculated from three independent assays. The selectivity index (SI), or the ratio of CC 50 to IC 50 , was also calculated to show the specificity of the drug's inhibitory effect.…”

Molecular mechanism of a specific capsid binder resistance caused by mutations outside the binding pocket

“…Plaque reduction assays were performed (with approximately 30-40 plaque-forming units of wt-CVB3 97927 or its variants and up to 8.0 µg/ml of pleconaril) and cytopathic effect (CPE) inhibition assays were performed in HeLa cell monolayers as described previously (Schmidtke et al, 2001) (Schmidtke et al, 2001 Sequence analysis of the genome region P1 coding for capsid proteins (purified PCR products) was performed with the Beckman Coulter Genom-Lab System (PubMed GenBank number: JX946654 and JX946654) following the manufacturer's instructions. Sequencing primers are summarized in Table S1.…”

“…Plaque reduction assays (with approximately 30-40 plaque-forming units of wt-CVB3 97927 or its variants and up to 8.0 µg/ml of pleconaril) and cytopathic effect (CPE) inhibition assays were performed in HeLa cell monolayers as described previously (Schmidtke et al, 2001). …”

Initial evidence on differences among Enterovirus 71, Coxsackievirus A16 and Coxsackievirus B4 in binding to cell surface heparan sulphate

“…The colour change was then recorded using a microplate reader at 540 nm. The virus inhibition percentages were measured using the following equation: T -V c /C c -Vc, where T = the optical density (OD) of compound-treated cells, V c = OD of virus control, C c = OD of cell control [19]. The antiviral activity curve was then generated by plotting virus inhibition percentages against compound concentrations.…”