A rapid, antibiotic susceptibility test for multidrug-resistant, Gram-negative bacterial uropathogens using the biochemical assay, DETECT

Jackson, Nicole; Borges, Clarissa Araújo; Tarlton, Nicole J.; Resendez, Angel; Milton, Aubrianne K; Boer, Tara R. de; Butcher, Cheyenne R.; Murthy, Niren; Riley, Lee W.

doi:10.1016/j.mimet.2021.106160

Cited by 12 publications

(13 citation statements)

References 53 publications

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“…The increasing prevalence of multidrug-resistant bacterial strains, particularly for the most common clinical pathogen P. aeruginosa in human isolates, presents a significant challenge in the identification of new treatment strategies, which can result in high rates of morbidity and mortality in hospitals and other healthcare facilities. Fortunately, a number of rapid methods have been developed to identify such MDR species and antibiotic susceptibility within a couple of hours from clinical isolates that have benefited from the reduction in increasing mortality [ 32 – 35 ]. Common characteristics for P. aeruginosa to represent the MDR behavior in clinical strains are the presence of drug resistance genes or its mutant variety in bacterial plasmid or in its genome, high expression of a β-lactamase group of enzymes, and up-regulation of drug efflux pump have been documented [ 36 – 39 ].…”

Section: Discussionmentioning

confidence: 99%

Association of multidrug resistance behavior of clinical Pseudomonas aeruginosa to pigment coloration

et al. 2022

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Pseudomonas aeruginosa is an adaptable bacterial pathogen that infects a variety of organs, including the respiratory tract, vascular system, urinary tract, and central nervous system, causing significant morbidity and mortality. As the primary goal of this study, we wanted to determine how pigment color production differed between clinical strains of P. aeruginosa, and whether or not that variation was associated with multidrug resistance or the ability to form biofilms. We screened in total 30.1% of yellow, 39.8% green and 30.1% of no pigment-producing P. aeruginosa strains from a total of 143 various clinical isolates. Yellow pigment-producing strains presented significant resistance to antibiotics groups, including β-lactam (91.5%), aminoglycosides (70.5%), and carbapenems (51.9%) compared to green and non-pigmented strains. Notably, 16.3% of yellow pigment-producing strains were resistant to colistin which is used as a last-resort treatment for multidrug-resistant bacteria, whereas only 2.3% of non-pigmented and 1.8% of green pigmented strains were resistant to colistin. Aside from that, yellow pigment-producing strains were frequent producers of enzymes belonging to the lactamase family, including ESBL (55.6%), MBL (55.6%), and AmpC (50%). Compared to the green groups (7.14%) and non-pigmented groups (28.5%), they had a higher frequency of efflux positive groups (64.2%). Notably, when compared to non-pigmented groups, green pigment-producing strains also displayed antibiotic susceptibility behavior similar to yellow pigment-producing strains. The majority of yellow pigment-producing strains outperformed the green and non-pigmented strains in terms of MIC levels when compared to the other two groups of strains. Despite the fact that previous studies have demonstrated a direct correlation between multidrug resistance behaviors and biofilm production, no such statistically significant association between pigment and biofilm formation was found in our investigation. Our research has demonstrated that the correlation of bacterial pigments on their susceptibility to antimicrobial agents. Yellow pigment-producing P. aeruginosa strains posed a significant problem due to the lack of alternative agents against such transformed strains, which may be associated with the development of multidrug resistance. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Association of multidrug resistance behavior of clinical Pseudomonas aeruginosa to pigment coloration

et al. 2022

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“…The increasing incidence of MDR bacterial strains particularly for most common clinical pathogen P. aeruginosa in human isolates poses a significant challenge to identify new treatment strategies that can lead to high morbidity and mortality in hospitals. Fortunately, a number of rapid methods have been developed to identify such MDR species along with antibiotic susceptibility within a couple of hours from clinical isolates that have benefited to reduce increasing mortality [32][33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Multidrug Resistance Behaviors of Clinical Pseudomonas aeruginosa Strains Associated to Pigment Coloration

Kothari¹,

Yadav²,

Rajput³

et al. 2021

Preprint

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Pseudomonas aeruginosa is an adaptable bacterial pathogen that infects various organs, including the respiratory tract, vascular system, urinary tract, and central nervous system leading to high morbidity and mortality. Our primary focus of this study was to characterize P. aeruginosa clinical strains on the basis of pigment color production, determine its association to multidrug resistance behavior and ability to form biofilm. We identified yellow (30.1%), green (39.8%) and no pigment (30.1%) producing strains from a total of 143 clinical isolates. Yellow pigment producing strains presented significant resistance to a class of antibiotics including β-lactam (91.5%), aminoglycosides (70.5%), and carbapenems (51.9%) compared to green and non-pigmented strains. Importantly, 16.3% of yellow pigment producing strains was resistant to colistin where only 2.3% of non-pigmented and 1.8% of green pigmented strains were resistant to this agent. Moreover, yellow pigment producing strain were frequent producers of β-lactamase group of enzymes, ESBL (55.6%), MBL (55.6%), and AmpC (50%) and displayed higher frequency of efflux positive group (64.2%) compared to green (7.14%) and non-pigmented one (28.5%). Notably, green pigment producing strains when compared to non-pigmented groups also displayed antibiotic susceptibility behavior similar to yellow pigment producing strains. Although yellow pigment producing strains were strong biofilm producers, no significant association was identified between pigment and biofilm formation. Among pigmented and non-pigmented strains, majority of yellow pigment producing strains have shown MIC levels greater than the green and non-pigmented strains. Our study has demonstrated the impact of pigment coloration on susceptibility to antimicrobial agents where yellow pigment producing strains represent considerably a serious problem as due to lack of alternative agents against such transformed strain may collectively be associated with multidrug resistance development.

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“…One significant concern is the resistance of Enterobacteriaceae to later generations of cephalosporins due to the acquisition of plasmids encoding extended-spectrum β-lactamases (ESBLs) [3]. The treatment options for ESBL producing bacteria are limited as they confer resistance to many available antibiotics [4]. The most common of the ESBL variants is the CTX-M group of enzymes capable of hydrolyzing third-generation cephalosporins such as cefotaxime.…”

Section: Introductionmentioning

confidence: 99%

Application of a solid-phase microextraction-gas chromatography-mass spectrometry/metal oxide sensor system for detection of antibiotic susceptibility in urinary tract infection-causing Escherichia coli – A proof of principle study

Drabińska

Hewett

White

et al. 2022

Advances in Medical Sciences

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A rapid, antibiotic susceptibility test for multidrug-resistant, Gram-negative bacterial uropathogens using the biochemical assay, DETECT

Cited by 12 publications

References 53 publications

Association of multidrug resistance behavior of clinical Pseudomonas aeruginosa to pigment coloration

Association of multidrug resistance behavior of clinical Pseudomonas aeruginosa to pigment coloration

Multidrug Resistance Behaviors of Clinical Pseudomonas aeruginosa Strains Associated to Pigment Coloration

Application of a solid-phase microextraction-gas chromatography-mass spectrometry/metal oxide sensor system for detection of antibiotic susceptibility in urinary tract infection-causing Escherichia coli – A proof of principle study

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