A rapid and simple UHPLC-ESI-MS/MS method for the screening of propyl propane thiosulfonate, a new additive for animal feed

Abad, Paloma; Arroyo‐Manzanares, Natalia; Garcı́a-Campaña, Ana M.

doi:10.1039/c6ay00219f

Cited by 16 publications

(19 citation statements)

References 29 publications

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“…The MS was working with ESI in positive mode (ESI+) under MRM conditions (Table 3). The target scan time established for each transition was 0.1 s. The ionization source parameters were: source temperature 500 • C; curtain gas (nitrogen) 30 psi; ion spray voltage 5500 V; and GAS 1 and GAS 2 (both of them nitrogen) were set to 50 psi (Abad et al, 2016). Under these conditions, CSSP, GSSP and PTSO were determined in 4.0 min.…”

Section: Microbiological Determinationsmentioning

confidence: 99%

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Effect of Allium Extract Supplementation on Egg Quality, Productivity, and Intestinal Microbiota of Laying Hens

Abad¹,

Arroyo‐Manzanares

Ariza³

et al. 2020

Animals

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The use of allium extract containing propyl propane thiosulfonate (PTSO) as hen feed supplement was evaluated to demonstrate its positive effect on egg production and intestinal microbiota modulation. The study was carried out on 90 laying hens whose feed was supplemented with allium extract for 28 days. Nutritional properties of eggs were not affected, whereas an improvement in productivity was observed based on the increase weight of eggs. In addition, a modulator effect on intestinal microbiota was confirmed by the increase in Lactobacillus spp. and Bifidobacterium spp., as well as by the reduction in Enterobacteriaceae populations. Finally, the preservation of egg composition was checked by monitoring the content of PTSO, using a new analytical method consisting of the use of solid phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Consequently, based on current results, Allium spp. extract rich in organosulfur compounds such as PTSO added to the diet had a beneficial effect on the microbiota and would seem to be a possible alternative to increase productivity, while not affecting the biochemical composition of egg. However, further studies on the effects of allium extract as feed supplement are necessary.

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Section: Microbiological Determinationsmentioning

confidence: 99%

“…Finally, an analytical method was developed to control egg biochemical composition by monitoring PTSO residues [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Allium Extract Supplementation on Egg Quality, Productivity, and Intestinal Microbiota of Laying Hens

Abad¹,

Arroyo‐Manzanares

Ariza³

et al. 2020

Animals

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“…The analysis was performed according to the method previously development in our lab. 23 Under these conditions, PTSO, CSSP, and GSSP were determined in 5.5 min, being characterized by two precursor−product ion transitions.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Previously, in our lab, several analytical methods for PTSO analysis in animal feed were developed but none of them for milk or its derivative products. 22,23 Thus, analytical methods for dairy product quality control should be developed. As stated above, the presence of PTSO in milk samples could alter its organoleptic properties, such as taste and flavor.…”

Section: ■ Introductionmentioning

confidence: 99%

Use of Onion Extract as a Dairy Cattle Feed Supplement: Monitoring Propyl Propane Thiosulfonate as a Marker of Its Effect on Milk Attributes

Abad¹,

Arroyo‐Manzanares

Gil³

et al. 2017

J. Agric. Food Chem.

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Onion extract is used as a feed supplement for the diet of dairy cows, acting as inhibitor of methane production; however, its properties could alter sensory attributes of milk. In this work, we propose a method to evaluate the influence of this extract on milk properties, using propyl propane thiosulfonate (PTSO) as a marker. PTSO is extracted using a quick, easy, cheap, effective, rugged, and safe procedure and monitored by high-performance liquid chromatography with ultraviolet detection. The method was applied to milk samples obtained from 100 dairy cows fed during 2 months with enriched feed. In addition, a milk tasting panel was established to evaluate the PTSO residue that should not be exceeded to guarantee milk sensory attributes. It was established that a value of PTSO lower than 2 mg kg does not alter milk organoleptic properties. This fact makes onion extract an interesting alternative as a feed supplement to control the methane emissions without any influence on milk attributes.

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“…In addition, to ensure the concentration of PTSO in feeds, UHPLC-ESI-MS/MS analyses were performed, according to the method described by Abad et al [33].…”

Section: Feed Serum and Feces Analysesmentioning

confidence: 99%

Effect of Alliaceae Extract Supplementation on Performance and Intestinal Microbiota of Growing-Finishing Pig

Sánchez

Ariza²,

Sánchez

et al. 2020

Animals

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The aim of the present study was to ascertain whether an Allium spp. extract rich in organosulfur compounds, such as propyl thiosulfonate (PTSO), added to the feed of growing-finishing pigs at 5 g/kg enhances growth performance or affects the fecal microbiome, the levels of short-chain fatty acids, or the antioxidant capacity of the animals. Fifty male growing pigs (large white) of 23.07 ± 2.87 kg average body weight were randomly allotted to two treatments in a 103-day trial. The trial was divided into two periods, an initial growing phase (56-days) and a finishing phase (47-days). Two dietary treatments for each phase (growing and finishing) were used: a control diet (CON) and an experimental diet consisting of the control diet to which 5 g/kg of Allium spp. extract was added to substitute sepiolite (GAR). Throughout the study, body weight, average daily gain (kg/day, ADG), feed intake (kg/day), and feed conversion ratio (kg/kg) were measured, while the backfat thickness and muscle depth were determined at the end of the study. Besides, feces samples were taken for bacterial counts by means of real-time PCR and short-chain fatty acid (SCFA) profile determination, and the antioxidant capacity was assessed in serum and saliva. In the animals receiving Allium spp. extract (5 g/kg) in the feed, ADG increased (p < 0.05) throughout the trial, Salmonella spp. and Clostridium spp. counts in feces had decreased (p < 0.05) when measured on day 56, and, by day 103, Salmonella spp., Clostridium spp., and Enterobacteriaceae counts had decreased (p < 0.05) and Lactobacillus spp. counts had increased (p < 0.01) in feces. Regarding the SCFA profile in feces and antioxidant capacity measured in serum and saliva, supplementation with Allium spp. extract significantly increased the levels of propionic, isobutyric, and isovaleric acids and the percentage of total branched fatty acids, while the c2/c3 and (c2 + c4)/c3 ratios were lower (p < 0.05) in feces; the Trolox equivalent antioxidant capacity and the cupric reducing antioxidant capacity levels in serum were significantly higher in the same pigs on day 103 than on day 0. Consequently, based on the current results, Allium spp. extract rich in organosulfur compounds, added to the diet at 5 g/kg, had a beneficial effect on the microbiota and would seem to be a possible alternative for increasing the growth performance of growing-finishing pigs. However, further studies on the effects of Allium spp. supplementation on carcass quality are necessary.

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A rapid and simple UHPLC-ESI-MS/MS method for the screening of propyl propane thiosulfonate, a new additive for animal feed

Abstract: Allium derivative propyl propane thiosulfonate (PTSO) is a new additive for animal feed which improves animal health when used as an alternative to antibiotic growth promoters.

Cited by 16 publications

References 29 publications

Effect of Allium Extract Supplementation on Egg Quality, Productivity, and Intestinal Microbiota of Laying Hens

Effect of Allium Extract Supplementation on Egg Quality, Productivity, and Intestinal Microbiota of Laying Hens

Use of Onion Extract as a Dairy Cattle Feed Supplement: Monitoring Propyl Propane Thiosulfonate as a Marker of Its Effect on Milk Attributes

Effect of Alliaceae Extract Supplementation on Performance and Intestinal Microbiota of Growing-Finishing Pig

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