A Rapid and Simple Method for Microscopy-Based Stomata Analyses

Eisele, Jochen F.; Fäßler, Florian; Bürgel, Patrick; Chaban, Christina

doi:10.1371/journal.pone.0164576

Cited by 71 publications

(54 citation statements)

References 21 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ImageJ was used to analyze microscopic images 1 and150 stomata were observed from the 15 images. The stomatal aperture was measured by the width of inner pore, and the proportion of open stomata was calculated by division of the number of open stomata by that of total stomata (Eisele et al, 2016).…”

Section: Stomatal Phenotype Observation and Measurementmentioning

confidence: 99%

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

et al. 2020

View full text Add to dashboard Cite

Salt stress impedes plant growth and development, and leads to yield loss. Recently, a halophyte species Mesembryanthemum crystallinum has become a model to study plant photosynthetic responses to salt stress. It has an adaptive mechanism of shifting from C 3 photosynthesis to crassulacean acid metabolism (CAM) photosynthesis under stresses, which greatly enhances water usage efficiency and stress tolerance. In this study, we focused on investigating the morphological and physiological changes [e.g., leaf area, stomatal movement behavior, gas exchange, leaf succulence, and relative water content (RWC)] of M. crystallinum during the C 3 to CAM photosynthetic transition under salt stress. Our results showed that in M. crystallinum seedlings, CAM photosynthesis was initiated after 6 days of salt treatment, the transition takes place within a 3-day period, and plants became mostly CAM in 2 weeks. This result defined the transition period of a facultative CAM plant, laid a foundation for future studies on identifying the molecular switches responsible for the transition from C 3 to CAM, and contributed to the ultimate goal of engineering CAM characteristics into C 3 crops.

show abstract

Section: Stomatal Phenotype Observation and Measurementmentioning

confidence: 99%

Physiological Changes in Mesembryanthemum crystallinum During the C3 to CAM Transition Induced by Salt Stress

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To measure the stomatal apertures of tomato plants pre-treated with B. amyloliquefaciens 54 or its derivatives, fully expanded tomato leaves were detached, and then incubated in MES buffer (pH 6.2) for detection [76]. Stomatal aperture images were recorded under microscopy (Zeiss Axio Observe 3m and Zen Standard Software, Carl Zeiss AG, Oberkochen, Germany).…”

Section: Stomata Assaysmentioning

confidence: 99%

Biofilms Positively Contribute to Bacillus amyloliquefaciens 54-induced Drought Tolerance in Tomato Plants

Wang

Jiang

Zhang

et al. 2019

IJMS

View full text Add to dashboard Cite

Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus amyloliquefaciens 54, from 30 potential PGPR to induce drought tolerance in tomato plants was examined via greenhouse screening. The results indicated that B. amyloliquefaciens 54 significantly enhanced drought tolerance by increasing survival rate, relative water content and root vigor. Coordinated changes were also observed in cellular defense responses, including decreased concentration of malondialdehyde and elevated concentration of antioxidant enzyme activities. Moreover, expression levels of stress-responsive genes, such as lea, tdi65, and ltpg2, increased in B. amyloliquefaciens 54-treated plants. In addition, B. amyloliquefaciens 54 induced stomatal closure through an abscisic acid-regulated pathway. Furthermore, we constructed biofilm formation mutants and determined the role of biofilm formation in B. amyloliquefaciens 54-induced drought tolerance. The results showed that biofilm-forming ability was positively correlated with plant root colonization. Moreover, plants inoculated with hyper-robust biofilm (∆abrB and ∆ywcC) mutants were better able to resist drought stress, while defective biofilm (∆epsA-O and ∆tasA) mutants were more vulnerable to drought stress. Taken altogether, these results suggest that biofilm formation is crucial to B. amyloliquefaciens 54 root colonization and drought tolerance in tomato plants.

show abstract

“…To see stomata and measure the sizes of their openings, leaf surfaces may be examined by making varnish replicas or by examining the epidermis of the leaf after it has been peeled or shaved from the leaf (Figure 2). In some cases, stains and other chemical treatments are used [10]. There are many videos and written instructions for these methods available on the Internet.…”

Section: Methodsmentioning

confidence: 99%