A Rapid and Sensitive PCR-Based Assay for Concurrent Detection of Bacteria Causing Common and Halo Blights in Bean Seed

Occurrence and Importance of Xanthomonas axonopodis pv. Phaseoli in Common Bean (Phaseolus vulgaris L) Seed Produced under Different Seed Production System in Central Rift Valley of Ethiopia

“…Although several techniques like serology [29,30] and polymerase chain reaction [31,32] have been developed for detection of CBB pathogen in seed, isolation on semis elective media remains the most widely used detection method [33][34][35]. In the present study, the morphological characteristics of the bacterium on the XCP1 medium, biochemical and pathogenicity test results confirmed that the pathogen recovered from seed was X. axonopodis pv.…”

Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds

“…PCR products were amplified for seeds from PI-80 and PI-50, but not for PI-10. In these seed samples, no amplification was found for the longest soaking time (72 h) for the PI-80 sample, but it was found between 1 and 12 h for the PI-50 sample (Table 1) Difficulties associated with PCR inhibitors when using seed-soaking liquid have been reported and they require a DNA extraction step prior to PCR (Audy et al, 1996;Kulik, 2008). Examples of assays that required a DNA extraction step include a real-time PCR assay to detect S. sclerotiorum on oilseed rape (Brassica napus L.) petals (Yin et al, 2009) and a qPCR assay to detect airborne S. sclerotiorum ascospores (Rogers et al, 2009).…”

“…Alternatives, such as PCR-based assays, are time-effective, versatile, and sensitive in identifying low quantities of DNA and amplifying specific products (Mullis and Faloona, 1987); they have also been used to detect several seed-borne pathogens (Audy et al, 1996;Blakemore and Reeves, 2002;Jaccoud-Filho et al, 2002;Taylor et al, 2006;Landa et al, 2007;Kulik, 2008;Jaccoud-Filho and Dabul, 2011). Due to its high specificity, PCR has been used to identify races of some fungal species, as shown by Jiménez-Gasco and Jiménez-Díaz (2003) for some races of Fusarium oxysporum f. sp.…”

Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds

“…A combination of specific primers was used to detect two bacteria specifically, but the other tested pathogenic bacteria did not give positive results. The method is sensitive, can detect as few as 1 infected seed in 10.000 seeds, and has a great potential for the detection of these two bacteria in commercial seeds (Audy et al, 1996). The presence of Psp was recorded on only two out of 23 common bean seed samples analyzed from the Novi Sad area in Serbia.…”

“…Various PCR assays have been proposed for the detection of Psp (Prosen et al, 1993;Schaad et al, 1995Schaad et al, , 2001Mosqueda and Herrera, 1997). All of them rely on the detection of DNA sequences involved in the biosynthesis of phaseolotoxin (Prosen et al, 1993;Schaad et al, 1995;Audy et al, 1996;Güven et al, 2004).…”