A rapid and sensitive method for simultaneous determination of insulin and A21-desamido insulin by high-performance liquid chromatography

Moslemi, Payam; Najafabadi, Abdolhossein Rouholamini; Tajerzadeh, Hosnieh

doi:10.1016/s0731-7085(03)00336-4

Cited by 47 publications

(43 citation statements)

References 23 publications

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“…Th e mean diameter of nanoparticles was determined by atomic force microscopy (Brucker Nano GmbH, Berlin, Germany). Th e amount of insulin entrapped within polymeric nanoparticles was determined by high performance liquid chromatography (HPLC) (Jasco 807-IT; JASCO, Tokyo, Japan) (Moslemi et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Oral delivery of insulin-loaded nanoparticles in diabetic rabbits and in sheep

Olya¹,

Khorvash²,

Rahmani³

et al. 2014

CZECH J ANIM SCI

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Th e objective of this study was to produce Eudragit ® RS 100 microparticles (ERS) containing insulin (ILNP), and to evaluate the potential of these nanoparticles as a drug carrier for oral administration in alloxan-induced diabetic rabbits and in sheep. After oral administration of ILNP to diabetic rabbits, a significant hypoglycemic eff ect was observed and this eff ect lasted for 2 days. Concentrations of blood glucose were signifi cantly decreased in ILNP-treated sheep compared to those in control and ERS groups on day 5 of the experiment. Orally ILNP-treated sheep exhibited higher concentrations of progesterone compared to control and ERS in all sampling times. Sheep in the ILNP group had lower concentrations of cortisol than the animals in the control group on days 1 and 3. However, other blood hormone parameters were not aff ected by the treatments. Th e results show that encapsulation of insulin into nanoparticles allows the preservation of its biological activity when going through digestive system of rabbit and sheep to the blood stream.

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Section: Methodsmentioning

confidence: 99%

Oral delivery of insulin-loaded nanoparticles in diabetic rabbits and in sheep

Olya¹,

Khorvash²,

Rahmani³

et al. 2014

CZECH J ANIM SCI

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“…The insulin HPLC analysis method was developed from a reported method [16]. Insulin was analysed by reversed phase-HPLC (Agilent LC1100 Series, Agilent Technologies; Germany) using a Jupiter 5 m C18 300Å column (150 4.60 mm; Phenomenex, Torrance, CA) maintained at 25ºC and a mobile phase consisting of 0.1% trifluoroacetic acid (TFA) in water and 0.1% TFA in ACN in a ratio of 70.5:29.5 (v/v) in the isocratic mode.…”

Section: Hplc Analysis Of Insulinmentioning

confidence: 99%

“…Like many peptides, insulin is unstable in an acid environment and transforms into side-products such as the hydrolysis product desamido-insulin [16]. In order to determine if the intrinsic instability of insulin could distort the results of the drug loading and in vitro release studies, insulin stability in the drug extraction and release mediums was investigated.…”

Section: Stability Of Insulinmentioning

confidence: 99%

Formulation of Nano and Micro PLGA Particles of the Model Peptide Insulin: Preparation, Characterization, Stability and Deposition in Human Skin

Wang¹,

Chen²,

Benson³

2008

TODDJ

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Abstract:The primary objective of this study was to develop a particulate formulation for peptide delivery that would provide enhanced peptide stability, controlled release and the potential for targeting to specific tissues. Biodegradable hydrophobic particles were prepared from poly (D,L-lactide-co-glycolide) (PLGA) by both solvent evaporation and solvent diffusion methods. Bovine insulin was chosen as a model peptide for formulation development and evaluation. By forming a complex between insulin and protamine, 50% incorporation of the model peptide in PLGA particles was achieved and a sustained release of insulin was observed over one week with improved stability of insulin in the PLGA matrix. The formation of an insulin-protamine complex and the method of manufacture are important determinants of the physicochemical characteristics of the particles formed. Preliminary evaluation of the deposition of the particles within skin was determined by fluorescent images following topical application to excised human skin. Microparticles with size above 7 μm remained on the surface of the skin. Nanoparticles (<1 μm) showed permeation into the viable epidermis and dermis with deposition concentrated around the hair follicles and sebaceous glands.

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“…Some instrumental analytical methods based on LC or CE were also developed for the analysis of pharmaceutical formulations but only for human insulin quantification in formulations without protamine [12][13][14][15]. Recently, CE and MEKC were applied to the separation of various insulin analogues [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of insulin and its analogues in pharmaceutical formulations by micellar electrokinetic chromatography

Lamalle

Servais

Radermecker

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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a b s t r a c tA simple and efficient MEKC method was developed to simultaneously determine human insulin, its five analogues, the main degradation products and the excipients usually present in injection formulations. A very fast method with a total analysis time of 3 min was then successfully validated for the analysis of human insulin and the quality control of commercial formulations was carried out.

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A rapid and sensitive method for simultaneous determination of insulin and A21-desamido insulin by high-performance liquid chromatography

Cited by 47 publications

References 23 publications

Oral delivery of insulin-loaded nanoparticles in diabetic rabbits and in sheep

Oral delivery of insulin-loaded nanoparticles in diabetic rabbits and in sheep

Formulation of Nano and Micro PLGA Particles of the Model Peptide Insulin: Preparation, Characterization, Stability and Deposition in Human Skin

Simultaneous determination of insulin and its analogues in pharmaceutical formulations by micellar electrokinetic chromatography

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