A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the estimation of rivastigmine in human plasma

Bhatt, Jignesh; Subbaiah, Gunta; Kambli, Sandeep; Shah, Bhavin; Nigam, Samita; Patel, Mehul; Saxena, Ashish; Baliga, Ashok; Parekh, Hetal; Yadav, Gunvat

doi:10.1016/j.jchromb.2007.01.003

Cited by 44 publications

(34 citation statements)

References 6 publications

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“…summation approach of multiple MRM transition pairs now being increasingly considered in the LC/MS/MS analysis to increase the sensitivity of many compounds (Beaudry and Vachon, 2009;Trivedi et al, 2008;Link et al, 2007;Bhatt et al, 2007;Nitin et al, 2003). Interestingly, for both compounds, one of the MRM transition pair (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, a higher sensitivity has been achieved for several important compounds and/or metabolites such as capsaicin (Beaudry and Vachon, 2009), torcetrapib (Trivedi et al, 2008), bulaquine and primaquine (Nitin et al, 2003), midazolam and 4-hydroxymidazolam (Link et al, 2007), and rivastigmine (Bhatt et al, 2007) by the application of the summation of response of multiple transition pairs. In an elegant overview on this topic, several important considerations during the validation of assays involving summation of transition pairs have been discussed.…”

Section: Introductionmentioning

confidence: 99%

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Sensitivity enhancement and matrix effect evaluation during summation of multiple transition pairs—case studies of clopidogrel and ramiprilat

Swamy

Kamath

Shekar

et al. 2009

Biomedical Chromatography

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Sensitivity enhancement via summation of multiple MRM transition pairs is gaining popularity in tandem mass spectrometric assays. Numerous validation experiments describing the assays for two model substrates, clopidogrel and ramiprilat, were performed. The quantitation of clopidogrel was achieved by the summation of transition pairs m/z 322.2 to m/z 212.0 and m/z 322.2 to m/z 184.0, while that of ramiprilat was achieved by the summation of transition pairs m/z 389.2 to m/z 206.1 and m/z 389.2 to m/z156.1. The use of summation approach achieved sensitivities of >2 fold for both compounds as compared with the reported single MRM transition pair assays. The validation experiments addressed some important assay development issues, such as: (a) lack of impact of matrix effect; (b) unequivocal verification of the percentage contribution of each MRM transition pair towards sensitivity; (c) sensitivity enhancement factor achieved by summation approach of MRM transition pairs; and (d) accurate prediction of quality control samples using summation approach vs a single MRM transition pair. In summary, the appropriateness of using two MRM transition pairs for quantitation was demonstrated for both clopidogrel and ramiprilat. Additionally, pharmacokinetic application of the MRM transition pair assays using a summation approach was established for the two compounds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sensitivity enhancement and matrix effect evaluation during summation of multiple transition pairs—case studies of clopidogrel and ramiprilat

Swamy

Kamath

Shekar

et al. 2009

Biomedical Chromatography

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“…The most important ones are the reduction of the reagents cost, the low injection volume requirements, and the avoidance of disposing large volumes of organic waste. In particular, when the study described here is compared to previous studies, where HPLC-MS and GC-MS were used for the analysis of rivastigmine, the required injection volume of plasma for a single analysis is reduced from microliters [40][41][42][43] to nanoliters.…”

Section: Applicationmentioning

confidence: 99%

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Kapnissi‐Christodoulou¹

2012

Electrophoresis

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In addition, hydrogen bonding, dipole-dipole, and dispersive interactions can contribute to the solute partitioning between the two phases [4][5][6].CE was originally considered as a powerful analytical tool for the analysis of biological macromolecules. It has though, over the years, been extensively used for the separation of other compounds, such as chiral drugs, food additives, pesticides, inorganic ions, organic acids, and others. In this chapter, the ability of CE, and particularly CZE and MEKC, to be used for the qualitative and quantitative determination of compounds in pharmaceutical, biological and natural samples is investigated. In each approach, a number of studies are reported and discussed. These studies involve establishment of optimum separation conditions, method validation, optimization of sample-preparation procedure and application for the determination of the analytes under study in real samples. The first part of this chapter involves the determination of polyphenolic compounds using CZE with UVVis detector in red and white wines, while the second part involves the determination of pharmaceutical compounds in biological samples, such as blood and urine, using the hyphenated technique CE-MS (mass spectrometry). The third and final part emphasizes the importance of MEKC in chiral analysis since it has been known that usually only one enantiomer is active, while the other may be less active, inactive or has adverse effects. Determination of polyphenolic compounds in natural samplesPolyphenolic compounds exist in a variety of natural products, such as fruits, vegetables, beverages (tea, wine and juices), honey, cacao and herbs. They attract a lot of interest due to their beneficial implication in human health. They have been widely studied due to their antioxidant capacity and their association with several pathological conditions, such as hypertension, cardiovascular disease, dementia, and even cancer [7][8][9]. Therefore, due to their health significance, numerous analytical methods have, over the last decades, been developed for their separation, identification and quantitation in natural products [10][11][12][13].According to literature, the simplest CE method, CZE, proved to be the best method for the determination of polyphenolic compounds in wine samples [14][15][16][17]. In such studies, and in each case, when the optimum CZE method was applied to different red and white wines, it was established that red wines have higher levels of polyphenolic compounds than white wines and that the polyphenolic composition varies among different wines.

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“…The use of this concept increased the sensitivity and was found to give a reproducible linear response over the calibration range with targeted LLOQ. The approach of using two product ions for quantitation was reported earlier by Nitin et al (2003) for the quantitation of bulaquine and primaquine in monkey plasma, Bhatt et al (2007) for the estimation of rivstigmine in human plasma and Link et al (2007) for the quantitation of midazolam…”

Section: Mass Spectrometrymentioning

confidence: 99%

Development and validation of a sensitive LC‐MS/MS method with electrospray ionization using multiple ions for quantitation of torcetrapib in hamster and dog plasma

Trivedi¹,

Dubey²,

Mullangi³

et al. 2007

Biomedical Chromatography

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A highly sensitive and specific LC-MS/MS method has been developed and validated for the estimation of torcetrapib (TTB) with 100 microL hamster/dog plasma using DRL-16126 as an internal standard (IS). The API-4000 Q Trap LC-MS/MS was operated under multiple-reaction monitoring mode using the electrospray ionization technique. The assay procedure involved extraction of TTB and IS from plasma with acetonitrile, which yielded consistent recoveries of 65.73 and 94.01% for TTB and 79.68 and 90.70% for IS in hamster and dog plasma, respectively. The total chromatographic run time was 3.0 min and the elution of TTB and IS occurred at approximately 2.25 and 2.20 min, respectively. The resolution of peaks was achieved with 0.01 m ammonium acetate:acetonitrile (15:85, v/v) at a flow rate of 0.40 mL/min on an Inertsil ODS-3 column. The method was proved to be accurate and precise at linearity range of 1.00-200 ng/mL with a correlation coefficient (r) of > or = 0.993. The method was rugged with 1.00 ng/mL as the lower limit of quantitation. TTB was stable in the battery of stability studies. The application of the assay to preclinical pharmacokinetic studies confirmed the utility of the assay to derive hamster/dog pharmacokinetic parameters.

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A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the estimation of rivastigmine in human plasma

Cited by 44 publications

References 6 publications

Sensitivity enhancement and matrix effect evaluation during summation of multiple transition pairs—case studies of clopidogrel and ramiprilat

Sensitivity enhancement and matrix effect evaluation during summation of multiple transition pairs—case studies of clopidogrel and ramiprilat

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Development and validation of a sensitive LC‐MS/MS method with electrospray ionization using multiple ions for quantitation of torcetrapib in hamster and dog plasma

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