A rapid and sensitive HPLC–MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats

Yang, Xinye; Zhou, Guanshen; Zou, Pingping; Ning, Ying; Zan, Ke; Tu, Peng‐Fei; Jiang, Yong

doi:10.1016/j.jchromb.2011.07.002

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…2e), and one xanthone, polygalaxanthone III which illustrated [M-H] − at m/z 567.1335 as precursor ion and a series shown product ions was characterized by comparing with a reference standard (Fig. 2f) [18][19][20].…”

Section: Othersmentioning

confidence: 99%

Characterization of multiple constituents in Kai-Xin-San prescription and rat plasma after oral administration by liquid chromatography with quadrupole time-of-flight tandem mass spectrometry

Zhang

et al. 2015

J. Sep. Science

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A sensitive and reliable ultra high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry method was established to separate and identify the chemical constituents of Kai-Xin-San prescription, a classic traditional Chinese medicine formula that plays an important role in the treatment of Alzheimer's disease. The detection was performed on an Agilent 6520 Accurate-Mass quadrupole time-of-flight mass spectrometer equipped with an electrospray ionization source in negative modes. With the optimized conditions, a total of 54 compounds were identified or tentatively characterized. Out of the 54 compounds, six compounds were identified by comparing the retention time and mass spectrometry data with reference standards, the rest were characterized by analyzing mass spectrometry data and retrieving the literature data. Results indicated ginsenosides, polygala saponins, terpenoids, and oligosaccharide esters were the major effective constituents in Kai-Xin-San prescription. There were 26 prototype ingredients that were assigned for identification in rat plasma. It is also concluded that the developed ultra high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry method with high sensitivity and resolution is suitable for identifying and characterizing the chemical constituents of Kai-Xin-San prescription, and the analysis provides a helpful chemical basis for further research on Kai-Xin-San prescription and the clinical diagnostics of Alzheimer's disease.

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Section: Othersmentioning

confidence: 99%

Characterization of multiple constituents in Kai-Xin-San prescription and rat plasma after oral administration by liquid chromatography with quadrupole time-of-flight tandem mass spectrometry

Zhang

et al. 2015

J. Sep. Science

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“…Compared our results with the previous literature (Wang et al, ), the t 1/2 of demethylbellidifolin in rats after a single dose of pure substance was shorter than that of G. acuta extract (demethylbellidifolin, t 1/2 = 24.9 h), suggesting that other components in the extract may influence the pharmacokinetic behaviors of demethylbellidifolin. The absolute oral bioavailability of demethylbellidifolin in rats was low with a value of 3.6%, which was comparable with those of other xanthones, such as α ‐mangostin, neomangiferin, gambogic acid and sibiricaxanthone F (Li et al, ; Yang et al, ; Yang, Liu, Shang, Qin, & Xia, ; Zheng, Ou, Zhang, Li, & Li, ). Owing to the poor bioavailability of demethylbellidifolin, more attention should be paid to the in vivo metabolites of demethylbellidifolin that might exist in plasma in the further studies.…”

Section: Resultsmentioning

confidence: 64%

Validated LC–MS/MS method for quantitation of demethylbellidifolin in rat plasma and its application to pharmacokinetic and bioavailability studies

Zhang

Yan

2017

Biomedical Chromatography

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Demethylbellidifolin, a major xanthone compound of Swertia davidi Franch, shows many beneficial pharmacological effects including antioxidation, anti-inflammation, anti-fibrosis and cardiovascular protection effects. In this research, a rapid and sensitive LC-MS/MS method for the quantitative analysis of demethylbellidifolin in rat plasma was developed. The demethylbellidifolin and internal standard of aurantio-obtusin were extracted from 50 μL of rat plasma samples with ethyl acetate, then the dried residue was reconstituted and injected in an HPLC system with Zorbax SB-C analytical column (2.1 × 100 mm, 3.5 μm) and eluted with the mobile phase consisting of methanol and 0.2% formic acid aqueous solution (80:20, v/v). Quantification was performed using a TSQ Quantum Ultra mass spectrometer in negative ESI using selected reaction monitoring mode of the transitions m/z 259.1 → 215.1 for demethylbellidifolin and 329.0 → 314.2 for the IS. Excellent linearity was observed between 1.92 and 960 ng/mL with a limit of quantitation of 1.92 ng/mL. Intra- and inter-day precision (RSD) values of quality control samples were both <8.3%. This study was successfully utilized for the pharmacokinetic profiles of demethylbellidifolin in rats after oral or intravenous administration. The oral bioavailability of demethylbellidifolin was 3.6%.

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“…About the 92 compounds, there were 53 from Radix Polygalae, 36 from Ginseng Radixet Rhizoma, and 3 from Poria. Including 24 compounds were unambiguously identified by comparing the retention time and MS peaks with the reference standards; the other 68 compounds were identified using MassHunter Data Acquisition Workstation software to carefully study their MS spectra, MS/MS spectra and comparing with the literature data . Generally, a mass error below 5 ppm is regarded as acceptable certainty.…”

Section: Resultsmentioning

confidence: 99%

“…Including 24 compounds were unambiguously identified by comparing the retention time and MS peaks with the reference standards; the other 68 compounds were identified using MassHunter Data Acquisition Workstation software to carefully study their MS spectra, MS/MS spectra and comparing with the literature data. [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] Generally, a mass error below 5 ppm is regarded as acceptable certainty. The MS data of the compounds are listed in Table 2, and their chemical structures are shown in the Supporting Information.…”

Section: Uhplc-q-tof-ms Analysis Of Kxsmentioning

confidence: 99%

Identification and determination of the major constituents in Kai‐Xin‐San by UPLC‐Q/TOF MS and UFLC‐MS/MS method

Sui

et al. 2016

J. Mass Spectrom.

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In order to have overall chemical material information of Kai-Xin-San (KXS), the reliable ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometer (UHPLC-Q-TOF-MS) and ultra-fast liquid chromatography mass spectrometer (UFLC-MS/MS) methods were developed for the identification and determination of the major constituents in KXS. Moreover, the UHPLC-Q-TOF-MS method was also applied to screen for multiple absorbed components in rat plasma after oral administration of KXS. The UHPLC-Q-TOF-MS method was achieved on Agilent 6520 Q-TOF mass and operated in the negative ion mode. Good separation was performed on a ZORBAX Eclipse Plus C18 column with a gradient elution at a flow rate of 0.2 ml/min. A total of 92 compounds in KXS were identified or tentatively characterized based on their exact molecular weights, fragmentation patterns, and literature data. A total of 26 compounds including 23 prototype components and three metabolites were identified in rat plasma after oral administration of KXS. Then, 16 major bioactive constituents were chosen as the benchmark substances to evaluate the quality of KXS. Their quantitative analyses were performed by a triple quadrupole tandem mass spectrometer (MS/MS) operating in multiple-reaction monitoring mode(MRM). The analysis was completed with a gradient elution at a flow rate of 0.4 ml/min within 35 min. The simple and fast method was validated and showed good linearity, precision, and recovery. Furthermore, the method was successful applied for the determination of 16 compounds in KXS. All results would provide essential data for identification and quality control of active chemical constituents in KXS. Copyright © 2016 John Wiley & Sons, Ltd.

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A rapid and sensitive HPLC–MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats

Cited by 11 publications

References 25 publications

Characterization of multiple constituents in Kai-Xin-San prescription and rat plasma after oral administration by liquid chromatography with quadrupole time-of-flight tandem mass spectrometry

Characterization of multiple constituents in Kai-Xin-San prescription and rat plasma after oral administration by liquid chromatography with quadrupole time-of-flight tandem mass spectrometry

Validated LC–MS/MS method for quantitation of demethylbellidifolin in rat plasma and its application to pharmacokinetic and bioavailability studies

Identification and determination of the major constituents in Kai‐Xin‐San by UPLC‐Q/TOF MS and UFLC‐MS/MS method

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