A rapid and safe procedure for preparing the murine toxins of the plague bacillus (Yersinia pestis)

Seguin, M C; Harrison, Daniel N.; Williams, J. E.

doi:10.1016/0041-0101(87)90162-0

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…These results suggest that Y. pestis isolates in north-east Brazil display an overall similarity regarding the outer membrane electrophoretic profile and no clear geographic variants can be established based on this parameter. It should be noted that the classification of the Brazilian Y. pestis geographic variants carried out by Hudson and his group took into consideration the expression of the murine toxin, a cytoplasmic protein (3,6,17).…”

Section: Resultsmentioning

confidence: 99%

Electrophoretic characterisation of the outer membrane proteins ofYersinia pestisisolated in north-east Brazil

1989

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SUMMIARYThe outer membrane proteins of 38 Yersinia pestis isolates from all known plague foci of north-east Brazil were analysed by SDS-PAGE. Approximately 20 bands were consistently found in all strains analysed and 11 were selected for comparative studies. Although qualitative differences among the electrophoretic profiles of outer membrane proteins of wild Y. pestis isolates were not observed, quantitative alterations were clearly noted for most of these proteins. No particular quantitative alteration of the clectrophoretic profile of outer membrane proteins could be associated with the period of isolation and geographic origin of the isolates. The 64 kDa outer membrane protein was significantly expressed in higher amounts among Y. pestis strains isolated from a recent plague outbreak. The possible use of electrophoretic profiles of outer membrane proteins of wild Y. pestis isolates as a tool for epidemiological studies and for the analysis of virulence determinants is discussed.

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Section: Resultsmentioning

confidence: 99%

Electrophoretic characterisation of the outer membrane proteins ofYersinia pestisisolated in north-east Brazil

1989

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“…91001, EV, Otten, 0614F, and Tjiusidej(R) were 10 5 cells mL −1 , while MII and M23 yielded negative results. Y. pestis M23 is a F1-negative strain that lacks the virulence determinant of the F1 envelope antigen (Burrows and Bacon, 1958;Seguin et al, 1987). Although its F1 genes (caf1, caf1A, caf1M, and caf1R) are 100% identical to those of Y. pestis 91001, Y. pestis MII is an attenuated strain with fewer surface proteins than those of other Y. pestis vaccine strains (Lei et al, 1997).…”

Section: Inclusivity Evaluation For the Upt-lf Assaymentioning

confidence: 99%

Calibration of an Upconverting Phosphor-Based Quantitative Immunochromatographic Assay for Detecting Yersinia pestis, Brucella spp., and Bacillus anthracis Spores

Zhang

Zhao

et al. 2020

Front. Cell. Infect. Microbiol.

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Yersinia pestis, Brucella spp., and Bacillus anthracis are pathogens that can cause infectious zoonotic diseases with high mortality rates. An upconverting phosphor-based quantitative immunochromatographic (UPT-LF) assay, a point-of-care testing method suitable for resource-limited areas, was calibrated to quantitatively detect pathogenic bacteria. The bacterial purity or activity were ensured via staining methods and growth curves, respectively. Growth assays showed that the classic plate-counting method underestimated bacterial numbers compared with the bacterial counting method recommended by the reference material of the National Institutes for Food and Drug Control, China. The detection results of the UPT-LF assay differed significantly between the bacterial cultures in liquid and solid media and between different strains. Accelerated stability assessments and freeze-thaw experiments showed that the stability of the corresponding antigens played an important role in calibrating the UPT-LF assay. In this study, a new calibration system was developed for quantitative immunochromatography for detecting pathogenic bacteria. The results demonstrated the necessity of calibration for standardizing point-of-care testing methods.

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A rapid and safe procedure for preparing the murine toxins of the plague bacillus (Yersinia pestis)

Cited by 2 publications

References 11 publications

Electrophoretic characterisation of the outer membrane proteins ofYersinia pestisisolated in north-east Brazil

Electrophoretic characterisation of the outer membrane proteins ofYersinia pestisisolated in north-east Brazil

Calibration of an Upconverting Phosphor-Based Quantitative Immunochromatographic Assay for Detecting Yersinia pestis, Brucella spp., and Bacillus anthracis Spores

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