A rapid and reliable assay to determine flumequine, marbofloxacin, difloxacin, and sarafloxacin in commonly consumed meat by micellar liquid chromatography

Péris-Vicente, Juan; Iborra-Millet, Jesus; Albiol-Chiva, Jaume; Carda‐Broch, Samuel; Esteve‐Romero, Josep

doi:10.1002/jsfa.9314

Cited by 18 publications

(15 citation statements)

References 27 publications

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“…A stirring batch solid-to-liquid extraction [37] (SBSLE) procedure was used to extract the quinolones from the solid samples [7,32]. Five grams were introduced in an Erlenmeyer flask containing 50 mL of mobile phase; the solution was shaken in a magnetic stirrer for 1 h and ultrasonicated for 15 min.…”

Section: Sample Processingmentioning

confidence: 99%

“…The supernatant was decanted, filtered through a 0.45-µm Nylon membrane filter using a Büchner funnel with the help of a vacuum pump and injected. For fortified samples, the proper amount of quinolone standard solution was injected into the crushed sample, and the mixture was left for 24 h at room temperature to stimulate a slow elimination of the solvent and maximize the integration of the matrix and the analytes [32]. Therefore, these artificially contaminated samples were similar to the incurred ones.…”

Section: Sample Processingmentioning

confidence: 99%

“…General chromatographic conditions were taken from previously published methods about the determination of quinolone residues in food matrices such as fish flesh [29], honey [30,31], and meat [7,32]: flow rate, 1 mL/min; isocratic mode; non-controlled temperature; stationary phase, C18; surfactant, SDS; buffer, 0.01 M phosphate salt; pH, 3; sacrificial base, TEA at 0.5%; and the optimal values of fluorescence excitation/emission wavelength. The wavelength program used to register the signal was designed to determine each quinolone at their optimal detection conditions.…”

Section: Optimization Of the Chromatographic Conditionsmentioning

confidence: 99%

“…Therefore, they are washed harmlessly away to elute with the solvent front rather than precipitating into the column, reducing the probabilities of overlapping with the analytes. Besides, protein-bound drugs are released and can be determined [7,[29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

et al. 2019

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A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (R2 > 0.9991), limit of detection and decision limit (0.01–0.05 mg/kg), limit of quantification (0.025–0.150 mg/kg), detection capability (<0.4 times decision limit), trueness (−14.2% to +9.8%), precision (<14.0%), robustness, and stability. The procedure was environmentally friendly, safe, easy-to-conduct, inexpensive, and had a high sample throughput, thus it is useful for routine analysis as a screening method in a laboratory for food residue control.

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Section: Sample Processingmentioning

confidence: 99%

Section: Sample Processingmentioning

confidence: 99%

Section: Optimization Of the Chromatographic Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

et al. 2019

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“…The aim of the research was the development of a method based on micellar liquid chromatography (MLC) to determine MBZ in milk and other dairy products such as curd, butter, and cheese to check the absence of residues in the food and ensure consumer protection, and farming waste (urine and dung), since its presence in excretions is proof the drug has been administered to the bovine animal. MLC allows direct injection of samples into the chromatographic system without any pretreatment; using sodium dodecyl sulfate (SDS) as a surfactant in mobile phase makes the method rapid, sensitive, cost effective, and eco-friendly, as well as serves as an alternative to conventional HPLC system [20][21][22][23]. These are some of the main reasons behind selecting MLC to carry out the present research work.…”

Section: Introductionmentioning

confidence: 99%

Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

et al. 2020

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Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)-6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25-50 ppm), sensitivity (limit of detection 0.1-0.2 ppm; limit of quantification, 0.3-0.6 ppm), trueness (92.5-102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control.

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Solvent‐free mixed micellar mobile phases: An advanced green chemistry approach for reversed‐phase HPLC determination of some antihypertensive drugs

Ibrahim

Elmansi

Belal

2020

J of Separation Science

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Minimizing the amount of organic solvents without loss in chromatographic performance has been an important step toward greening analytical methodologies. Mobile‐phase composition is the key for maintaining separation efficiency in liquid chromatography while decreasing the procedure hazardousness. If sodium dodecyl sulfate is mixed with Brij‐35 in the mobile phase, they could be used as a green alternative for using organic modifiers. In this research, the effect of changing the relative amounts of both surfactants was studied on the chromatographic performance and separation efficiency of ten antihypertensive drugs belonging to different categories. The use of surfactants has many advantages including low cost and toxicity, safe environmental disposal, unique selectivity besides high solubilization capabilities. The optimum separation was maintained using a mobile phase (0.01 M Brij‐35, 0.08 M sodium dodecyl sulfate and 0.01 M sodium dihydrogen phosphate/pH 5) on reversed‐phase C18 core–shell column at flow rate 1.5 mL/min and temperature 30°C. The method was successfully applied for the determination of the drugs in various marketed dosage forms. International Conference of Harmonization guidelines were followed to validate the developed method. Additionally, the method was verified on the Green Analytical Procedure Index in regards to the greenness and found to be an excellent green alternative method.

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A rapid and reliable assay to determine flumequine, marbofloxacin, difloxacin, and sarafloxacin in commonly consumed meat by micellar liquid chromatography

Abstract: The method shows practical advantages such as simplicity, low cost, eco-friendliness, safety, and applicability for routine analysis, and is useful for surveillance programs. © 2018 Society of Chemical Industry.

Cited by 18 publications

References 27 publications

Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

Solvent‐free mixed micellar mobile phases: An advanced green chemistry approach for reversed‐phase HPLC determination of some antihypertensive drugs

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