A rapid and efficient in vitro shoot regeneration protocol using cotyledons of London plane tree (Platanus acerifolia Willd.)

Bao, Zhiru; Zhang, Yanping; Shao, Changsheng; Zhang, Jiaqi; Li, Guofeng; Bao, Manzhu

doi:10.1007/s10725-017-0303-2

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…Effect of PGRs in DM on direct regeneration was dependent on PGRs type and concentration (Bao et al 2017;, genotype (García-Forte et al 2020) and explant type (Geng et al 2023). According to Xu et al (2022a), CPPU and TDZ, two kinds of cytokinin with high activity, played remarkably positive roles in MNs induction and shoots regeneration from callus.…”

Section: Discussionmentioning

confidence: 99%

Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

fan,

li,

et al. 2024

Preprint

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Tissue culture is preferred for solving the shortcoming of low efficiency in terms of conventional propagation ways in tree peony, an economically important woody plant in China with various purposes. However, callus differentiation is hard to obtain during in vitro regeneration. Meristematic nodule (MN) is a favorable way capable of overcoming this problem, but possesses a lengthy process. Direct organogenesis excluding the callus step is needed to simplify the procedure. This study firstly presented a protocol of direct organogenesis and direct MNs induction and differentiation using cotyledon explant for in vitro regeneration of P.ostii ‘Feng Dan’. The highest direct MNs induction rate (41.67%) and frequency of direct organogenesis (DO) (66.67%) was achieved under the following procedure. The explants were pretreated in dedifferentiation induction medium (DIM) [Murashige and Skoog (MS) medium with 2.27 µMthidiazuron (TDZ)+5.37 µM α-naphthylacetic acid (NAA)] for 10 days, and then the cotyledons without callus induced were transferred to differentiation medium (DM) [Woody plant medium (WPM) containing 2.02 µM N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU)+2.27 µM TDZ and 4.04 µM CPPU+4.54 µM TDZ] respectively, with 6 subcultures, 90 days in total. The regenerated shoots rooted and transplanted successfully. Histological study conﬁrmed the process of DO and direct MNs induction, and revealed that shoots and MNs were originated from increased division of meristematic cell under cortical tissue, as well as from actively divided meristematic cells around vascular center. Moreover, shoots regenerated through MNs differentiation were originated from the epidermal and subepidermal cells. This study is an innovation and supplement in the field of in vitro regeneration in tree peony, and will be conductive to clonal micropropagation, fundamental studies of developmental biology and genetic transformation.

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Section: Discussionmentioning

confidence: 99%

Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

fan,

li,

et al. 2024

Preprint

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“…Considering the important role of the micropropagation technique in regeneration protocols, one of the goals of studies on tissue culture is to provide convenient and efficient methods for rapid organogenesis and mass production of plants under in vitro conditions, which can provide appropriate ground for other research areas such as genetic engineering . Previous studies on various plants such as Antirrhinum majus (Hesami and Daneshvar, 2016b), Tinospora cordifolia (Mridula et al, 2017), Platanus acerifolia (Bao et al, 2017), and Cucumis melo (Sebastiani and Ficcadenti 2016) have shown that cotyledon explants have a high potential for shoot proliferation. However, on the basis of the available literature, to date, there is no report on shoot proliferation of Mulberry by using cotyledon explants.…”

Section: Introductionmentioning

confidence: 99%

Effects of thidiazuron on in vitro shoot regeneration of Morus alba

Rezaei-Zafarghandi¹,

Rahmati‐Joneidabad²

2020

bta

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Mulberry (Morus alba L.) is an ornamental, medicinal, and fruit plant that belongs to the Moraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which enables mass production of pathogen-free plants. Cotyledon has a high potential for shoot regeneration; however, to the best of our knowledge, there are no reports on plant regeneration from cotyledon explants of mulberry. Therefore, this study aimed to evaluate the potential of seedling-derived cotyledon segments to obtain shoot multiplication of mulberry. Various concentrations (0, 0.25, 0.5, 1, 2, 3, and 4 mg/l) of thidiazuron (TDZ) in combination with indole butyric acid (IBA) were used in a completely randomized design in three replications. The results showed that the highest percentage of regeneration frequency (96.67%) and the maximum number of shoots (4.43) were obtained on Murashige and Skoog (MS) medium supplemented with 0.25 mg/l TDZ and 0.025 mg/l IBA. In the rooting experiment, the maximum rooting percentage (83.33%) and the maximum number of roots per shoot (4.36) were obtained on MS medium containing 2 mg/l IBA. In vitro-raised plantlets were placed in pots and kept in room temperature for 30 days, and the plantlets showed more than 90% survival rate. On the basis of our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.

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“…Capsicum and citrus). Here, in this special issue, Bao et al (2017) have developed a rapid and efficient in vitro shoot regeneration protocol for London plane tree (Platanus acerifolia Willd.) using cotyledons as explants.…”

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confidence: 99%

Plant genetic engineering and biotechnology: a sustainable solution for future food security and industry

OuYang

Holford

2017

Plant Growth Regul

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challenges associated with a changing climate. This special issue focuses on plant genetic improvement, especially on the tolerance of plants to stress.Three review papers are included in this special issue. Gerszberg and Hnatuszko-Konka (2017) present an excellent review of the genetic engineering of abiotic stress in tomato. Being adversely affected by various abiotic stresses, tomato provides an excellent research model for the study of these stress tolerance in fleshy fruits. Much effort has been made using genetic engineering to understand stress-related gene expression in tomato, and as such, deserves a comprehensive review. Unlike other reviews that divide this topic into different types of stress, this review focuses on the molecular players involved in stress responses. The review briefly introduces the physiological basis of abiotic stress tolerance in plants then details the approaches to genetic engineering and the achievements in understanding and improving stress tolerance. The review includes: (1) the genetic regulation of various metabolites, including mannitol, glycine betaine, glutathione, unsaturated fatty acids, osmotine, polyamines, and trehalose; (2) the regulation of genes associated with hormone pathways (e.g. ethylene), water channels (e.g. aquaporin) and ion transport; (3) the manipulation of heat shock proteins; (4) the regulation of enzymes in antioxidant systems, such as oxidoreductase, catalase, ascorbate peroxidase, and superoxide dismutase; and (5) the genetic transformation of regulatory genes (e.g. transcription factors and kinases) and other components of signaling systems (e.g. systemin, expansin, and late embryogenesis abundant protein).This review is likely to serve as an informative reference for abiotic stress research in tomato. Singh et al. (2016) present a short review of the roles of cross-talk between microRNA and nitric oxide (NO) in signaling associated with stress responses. MicroRNAs are Genetic engineering and biotechnology are becoming widely used in crop improvement and have provided a means by which increased yields of food and fiber can be produced in an environmentally sustainable manner. In addition, these techniques have allowed us to gain great insights into the networks of genes that result in the production of various bioproducts that can be beneficial for human health and environment. The past 5 years have witnessed substantial breakthroughs in plant genetic engineering and biotechnology. In addition to the manipulation of protein coding genes, microRNAs have proved to be promising targets in crop improvement, and the clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has emerged like a radiant sunrise, and has greatly facilitated the targeted modification of specific traits. Reverse breeding technologies are also providing a means of accelerating breeding, allowing new cultivars to be produced to combat the

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A rapid and efficient in vitro shoot regeneration protocol using cotyledons of London plane tree (Platanus acerifolia Willd.)

Cited by 6 publications

References 28 publications

Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

Effects of thidiazuron on in vitro shoot regeneration of Morus alba

Plant genetic engineering and biotechnology: a sustainable solution for future food security and industry

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