A rapid and consistent near infrared spectroscopic assay for biomass enzymatic digestibility upon various physical and chemical pretreatments in Miscanthus

Ammonium oxalate-extractable uronic acids positively affect biomass enzymatic digestibility by reducing lignocellulose crystallinity in Miscanthus

“…The procedure of plant cell wall fractionation was described by Peng et al (2000) with minor modification by Huang et al (2012). The well-mixed powder of biomass sample was initially ground under the potassium phosphate buffer (pH 7.0).…”

Diverse cell wall composition and varied biomass digestibility in wheat straw for bioenergy feedstock

“…Alkali (NaOH) pretreatment and enzymatic hydrolysis of the residues were performed as previously described by Huang et al [30] with minor modification. NaOH pretreatment: The well-mixed powder of biomass sample (0.3 g) was added with 6 mL 1% (w/v) NaOH, shaken at 150 rpm for 2 h at 50 C, and centrifuged at 3000 g for 5 min.…”

High-Throughput Profiling of the Fiber and Sugar Composition of Sugarcane Biomass

“…Applying these models to an at-line analyzer allows rapid, high-throughput prediction of lignocellulosic constituents for new samples [16]. The prediction of biomass composition using NIR spectral data has been developed for many species, such as Miscanthus [17], switchgrass [18], poplar [19], sugarcane (on three selected genotypes) [11], and in broad-based multispecies models (including corn, sorghum, rice, and wheat) [16]. In the Australian sugarcane industry, NIR spectroscopic models have been developed for evaluation of many sugar mill products, such as prepared cane, bagasse, raw sugar, molasses, juice and syrups, massecuite, magma, and mud [4,12,20].…”