A rapid analytical method for the quantification of paclitaxel in rat plasma and brain tissue by high‐performance liquid chromatography and tandem mass spectrometry

Abstract

This rapid LC/MS/MS method was validated to be sensitive, specific, precise and accurate for the quantification of paclitaxel in rat plasma and brain tissue homogenate. Application of the method to study samples provided sufficient proof of blood-brain barrier penetration of paclitaxel, allowing further investigation of its influence on the central nervous system.

Enhanced Oral Bioavailability of Morin Administered in Mixed Micelle Formulation with PluronicF127 and Tween80 in Rats

“…The concentrations of paclitaxel were analyzed using a modified LC-MS/MS method reported by Li et al 22) Briefly, Agilent 6430 Triple Quad LC/MS-MS system coupled with an Agilent 1260 series HPLC system was also used. The separation was performed on a Polar RP column (4.6 mm i.d.×150 mm, 5 µm, Phenomenex) using a mobile phase that consisted of methanol and DDW (90 : 10, v/v) with 0.1% formic acid at a flow rate of 0.2 mL/min.…”

An ultrasensitive LC–MS/MS method with liquid phase extraction to determine paclitaxel in both cell culture medium and lysate promising quantification of drug nanocarriers release in vitro

“…However, the addition of 0.1% formic acid to the mixture leads to a [M + H] + ion predominance. Further study showed that an acidic 2 mM ammonium acetate/water/ACN buffer, adjusted to pH 3.2 with formic acid, increased the signal-to-noise ratio of [M + H] + and suppressed the Na + adducted ion significantly [21][22][23][24][25]. For PTx, the base peak of the product ion spectrum of the [M + H] + (m/z 854.3) is detected at m/z 285.95.…”

“…This makes us thinking about to what extend LC-MS/MS reported methods were suitable to quantify PTx in biological medium such as complete cell culture medium supplemented with 10% of fetal bovine serum. It is worth noting that these methods recorded a high sensisitivity of PTx quantification ranging from 0.1 ng/mL to 0.2 pg/mL [19][20][21]24,25]. However, none of these methods is actually used to quantify PTx release from nanocarriers in complete cell culture medium, in order to determine the drug release profile before exposing to cells.…”

“…Indeed, due to its high hydrophobicity, most of in vitro studies of PTx release from nanocarriers were performed in simulated physiological medium supplemented with surfactant Tween 80 [11][12][13][14][15][16][17][18]. Numerous analytical methods using liquid phase extraction and solid phase extraction combined to the LC-MS/MS technique were developed for ultrasensitive quantifi- cation of PTx in biological samples such as cells, plasma, urine, feces or tissues [19][20][21][22][23][24][25][26][27][28][29][30]. Nevertheless, most of the published works use high-performance liquid chromatography or spectrophotometric UV-vis absorbance [11][12][13][14][15]17] and radio labeled [16] detection to determine the in vitro profile of PTx release from nanocarriers.…”

Enzymatic tumour tissue digestion coupled to SPE–UPLC–Tandem Mass Spectrometry as a tool to explore paclitaxel tumour penetration

“…Although mechanical tissue homogenization has proven to be reliable in e.g. the accurate quantification of brain PTX concentrations [9], in our application, given the vast heterogeneity in tumour tissue composition and tumour tissue density it was regarded impracticable. For example, several of the tumours we encountered contained a high degree of dense fibrous-tissue, making it impossible, using mechanical techniques, to homogenize the tumour to a satisfactory degree (i.e.…”