A rapid analysis for chemical grouping of aerobic actinomycetes.

Hasegawa, Takeshi; Takizawa, Masayuki; Tanida, Seiichi

doi:10.2323/jgam.29.319

Cited by 1,090 publications

(710 citation statements)

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“…Morphological properties were observed with a scanning electron microscope (model JSM-5600, JEOL). Isomers of diaminopimelic acid in whole-cell hydrolysates were determined by TLC following the standard methods of Becker et al 16 and Hasegawa et al 17 16S rDNA was amplified by PCR and sequenced directly on an ABI model 377A automatic DNA sequencer using a PRISM Ready Reaction Dye Primer Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA).…”

Section: Taxonomic Studiesmentioning

confidence: 99%

Panowamycins A and B, new antitrypanosomal isochromans produced by Streptomyces sp. K07-0010

et al. 2012

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Two new isochromans, panowamycins A and B, were purified by solvent extraction, silica gel and octadecylsilyl silica gel (ODS) column chromatography followed by preparative HPLC, from a culture broth of Streptomyces sp. K07-0010, together with the known compounds NFAT-133, conglobatin, piericidin C series and dinactin. Structures of panowamycins were elucidated as new analogs of NFAT-133 by spectroscopic studies including various NMR experiments. Panowamycins A and B showed moderate antitrypanosomal activity, with IC 50 values of 0.40 and 3.30 lg ml -1 , respectively.

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Section: Taxonomic Studiesmentioning

confidence: 99%

Panowamycins A and B, new antitrypanosomal isochromans produced by Streptomyces sp. K07-0010

et al. 2012

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“…The meso-isomer of diaminopimelic acid was detected by TLC using the method of Bousfield et al [32], and whole-cell sugars were analysed by the TLC method of Hasegawa et al [33]. The whole-cell sugars contained galactose, glucose, mannose and xylose.…”

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confidence: 99%

Micromonospora terminaliae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of the medicinal plant Terminalia mucronata

Kaewkla

Thamchaipenet

Franco

2017

International Journal of Systematic and Evolutionary Microbiology

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An endophytic actinobacterium, strain TMS7 T , was isolated from the stem of a Thai medicinal plant collected from the grounds of the Phujong-Nayoa National park, Ubon Ratchathani province, Thailand. As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Micromonospora. This strain was a Gram-stain-positive, aerobic actinobacterium with well-developed substrate mycelium with hyphae forming a single microspore was non-motile. Stran TMS7 T was identified according to its 16S rRNA gene sequence as a new member of the genus Micromonospora. The closest phylogenetic members sharing a similarity were Micromonospora chersina DSM 44151 T at 99.4 % and Micromonospora rosaria DSM 803 T , Micromonospora tulbaghiae TVU1 T , Micromonospora inositola DSM 43819 T and Micromonospora endolithica DSM 44398 T all at 99.2 %. Chemotaxonomic data including cell wall components, major menaquinones and major fatty acids confirmed the affiliation of strain TMS7 T to the genus Micromonospora. The results of the phylogenetic analysis, addition to physiological and biochemical studies in combination with DNA-DNA hybridization, allowed the genotypic and phenotypic differentiation of strain TMS7 T and the most closely related species with validly published names. The name proposed for the novel species is Micromonospora terminaliae sp. nov. The type strain is TMS7 T (=DSM 101760 T =NRRL B-65345 T ).

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“…The morphological properties were observed with a scanning electron microscope (model JSM-5600, JEOL, Akishima, Japan) after cultivation on 1/10 V8 juice agar at 27 1C for 18 days. Isomers of diaminopimelic acid in whole-cell hydrolysates were determined by TLC, following the standard methods of Becker et al 23 and Hasegawa et al 24 16S rDNA was amplified by PCR and sequenced directly on an ABI model 377A automatic DNA sequencer using a PRISM Ready Reaction Dye Primer Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA).…”

Section: Taxonomic Studiesmentioning

confidence: 99%

Sinefungin VA and dehydrosinefungin V, new antitrypanosomal antibiotics produced by Streptomyces sp. K05-0178

et al. 2010

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Two new nucleotide antibiotics, named sinefungin VA and dehydrosinefungin V, were separated by cation exchange column chromatography and purified by HPLC from the culture broth of Streptomyces sp. K05-0178, together with the known antibiotics, sinefungin, dehydrosinefungin and KSA-9342. The structures of the two novel sinefungin analogs were elucidated by spectroscopic studies, including various NMR and advanced peptide chemical methods. Sinefungin VA consists of adenosine and ornithylvalylalanine, whereas dehydrosinefungin V consists of 4¢,5¢-dehydroadenosine and ornithylvaline. Sinefungin VA showed potent antitrypanosomal activity with an IC 50 value of 0.0026 lg ml À1 in vitro without cytotoxicity against MRC-5 cells. Dehydrosinefungin V showed moderate antitrypanosomal activity (IC 50 ¼0.15 lg ml À1 ).

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A rapid analysis for chemical grouping of aerobic actinomycetes.

Cited by 1,090 publications

References 0 publications

Panowamycins A and B, new antitrypanosomal isochromans produced by Streptomyces sp. K07-0010

Panowamycins A and B, new antitrypanosomal isochromans produced by Streptomyces sp. K07-0010

Micromonospora terminaliae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of the medicinal plant Terminalia mucronata

Sinefungin VA and dehydrosinefungin V, new antitrypanosomal antibiotics produced by Streptomyces sp. K05-0178

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