A RAPD based study revealing a previously unreported wide range of mesophilic and thermophilic spore formers associated with milk powders in China

The heat resistance and spoilage potential of aerobic mesophilic and thermophilic spore forming bacteria isolated from Chinese milk powders

“…Table 1 The result of preliminary screening of the mesophilic and thermophilic isolates for five different enzymes on slective media. The RAPD pattern corresponds to previously published patter for these isolates (Sadiq et al, 2016) Table 2 Thermal screening of mesophilic and thermophilic sporeformers at 100°C, 110°C, 115°C, 120°C and 125°C for a fixed time period of 30 min. The value represents the means of three repetitions.…”

“…Lipolytic activity (p-nitrophenol/min/ml) of mesophilic and thermophilic sporeformers recovered from Chinese milk powders. The name of each isolate is followed by its RAPD pattern (Sadiq et al, 2016). For simplicity, only statistically different values are shown for the strains belonging to one RAPD group or sub-group.…”

“…The presence of a high number of T. vulgaris isolates has been reported twice in Chinese milk powders (Yuan et al, 2012;Sadiq et al, 2016).…”

“…In our previous study, we found a wide range of MP and TP spore-forming bacteria in Chinese milk powders (Sadiq et al, 2016) that belonged to 31 different RAPD groups and 24 species (including some groups). The heat resistance of many of these isolates and their potential to affect the quality of milk powders is unknown.…”

“…All representative strains of 31 different RAPD groups as well as sub-groups, representing 738 different isolates recovered from Chinese milk powders (Sadiq et al, 2016), were studied. A total of 178 strains (see Table 1) were randomly selected (1-10 from each RAPD group or sub-group, depending on the number of previously recovered strains belonging to a particular group) to determine their enzymatic potential based on screening on different selective media.…”