A Randomized Controlled Trial Comparing Live Birth From Single Euploid Frozen Blastocyst Transfer Using Standardized Timing Versus Timing by Endometrial Receptivity Analysis

Doyle, Nicole; Jahandideh, Samad; Hill, Micah J.; Widra, E.A.; Levy, Michael J.; Devine, Kate

doi:10.1016/j.fertnstert.2021.07.283

Cited by 10 publications

(3 citation statements)

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“…Although the timing for embryonic transfer is essential, it is not a limited time interval [ 49 ]. Recently, data from two large trials and a meta-analysis showed no clinical benefits associated with endometrial receptivity analysis of the WOI shift [ 50 , 51 , 52 ]. The nature of the WOI shift in different menstruation cycles may be governed by normal inter-cycle variation instead of pathological changes [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Cervical Secretion Methylation Is Associated with the Pregnancy Outcome of Frozen-Thawed Embryo Transfer

Lee

et al. 2023

IJMS

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The causes of implantation failure remain a black box in reproductive medicine. The exact mechanism behind the regulation of endometrial receptivity is still unknown. Epigenetic modifications influence gene expression patterns and may alter the receptivity of human endometrium. Cervical secretions contain endometrial genetic material, which can be used as an indicator of the endometrial condition. This study evaluates the association between the cervical secretion gene methylation profile and pregnancy outcome in a frozen-thawed embryonic transfer (FET) cycle. Cervical secretions were collected from women who entered the FET cycle with a blastocyst transfer (36 pregnant and 36 non-pregnant women). The DNA methylation profiles of six candidate genes selected from the literature review were measured by quantitative methylation-specific PCR (qMSP). Bioinformatic analysis of six selected candidate genes showed significant differences in DNA methylation between receptive and pre-receptive endometrium. All candidate genes showed different degrees of correlation with the pregnancy outcomes in the logistic regression model. A machine learning approach showed that the combination of candidate genes’ DNA methylation profiles could differentiate pregnant from non-pregnant samples with an accuracy as high as 86.67% and an AUC of 0.81. This study demonstrated the association between cervical secretion methylation profiles and pregnancy outcomes in an FET cycle and provides a basis for potential clinical application as a non-invasive method for implantation prediction.

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Section: Discussionmentioning

confidence: 99%

Cervical Secretion Methylation Is Associated with the Pregnancy Outcome of Frozen-Thawed Embryo Transfer

Lee

et al. 2023

IJMS

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“…Given the controversy generated by the aforementioned trial, a recent large randomized, controlled trial of the use of PET for transfer of euploid blastocysts was performed that avoided the heterogeneity of the first trial as well as the high dropout rates and differences between intention to treat and per protocol analyses. The trial was presented at the 2021 meeting of the American Society of Reproductive Medicine [85]. All patients underwent PGT-A and randomization occurred only if patients had at least one euploid blastocyst.…”

Section: Endometrial Receptivity Arraymentioning

confidence: 99%

Evaluation of endometrial receptivity and implantation failure

Bui

Timmons

Young

2022

Current Opinion in Obstetrics &Amp; Gynecology

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Purpose of reviewTo succinctly review the basic mechanisms of implantation and luteal phase endometrial differentiation, the etiologies of impaired endometrial function and receptivity, and the current methods that exist to evaluate and treat impaired endometrial receptivity. Recent findingsHuman embryo implantation requires bidirectional communication between blastocyst and a receptive endometrium. Etiologies of impaired endometrial receptivity are varied. Some of these include delayed endometrial maturation, structural abnormalities, inflammation, and progesterone resistance. Current methods to evaluate endometrial receptivity include ultrasonography, hysteroscopy, and endometrial biopsy. Treatments are limited, but include operative hysteroscopy, treatment of endometriosis, and personalized timing of embryo transfer.

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“…A large, randomized controlled trial demonstrated no benefit of ERA-guided embryo transfer in patients who underwent standard-timing frozen euploid blastocyst transfer over patients who underwent ERA-timed frozen euploid blastocyst transfer (3). The subject of this commentary, the study by Cozzolino et al (4) differed by evaluating patients who had a prior failed embryo transfer and also included donor egg cycles.…”

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confidence: 99%