A Randomized Comparison of Transcervical and Transabdominal Chorionic-Villus Sampling

Lg, Jackson; Jm, Zachary; Se, Fowler; Rj, Desnick; Ms, Golbus; Dh, Ledbetter; Mj, Mahoney; Pergament, Eugene; Jl, Simpson; Black, Susan H.

doi:10.1056/nejm199208273270903

Cited by 239 publications

(54 citation statements)

References 7 publications

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“…It was done under the ultrasound guidance either through trans abdominal or trans cervical route depending on the position of the placenta and choice of operating obstetrician [19]. Adequate sample was obtained through both the routes [20]. The adequacy of sample was confirmed both by visual inspection and under dissecting microscope.…”

Section: Cvs and Fetal Genotypementioning

confidence: 99%

Prenatal Diagnosis of Sickle Cell Disease by the Technique of PCR

Singh

Shrivastava

Shrikhande

2014

Indian J Hematol Blood Transfus

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Sickle cell disease (SCD) is prevalent in Central India and causes major morbidity and mortality. There is a lack of prenatal diagnostic facility near population affected with SCD. This is the pilot study in our region with the aim to establish prenatal diagnostic facility for the couples carrying sickle cell gene in Central India, in order to help them take an informed decision regarding fetus affected with SCD and also to calculate sensitivity of polymerase chain reaction (PCR) technique in our set up with follow up high performance liquid chromatography (HPLC) of baby's blood sample. Fetal sampling was done by chorionic villous biopsy. Extracted DNA was subjected to amplification refractory mutation system (ARMS-PCR) to detect sickle cell mutation (GAG ? GTG) in the sixth codon of b globin gene. Follow-up HPLC was done to detect baby's Hb pattern. Prenatal diagnosis of sickle cell anemia was offered in total 37 cases out of which one (2.7 %) fetal sample was inadequate. Total 26 (70.27 %) fetuses had AS Hb genotype, 3 (8.11 %) had AA Hb genotype and 3 (8.11 %) had SS Hb genotype while remaining 4 (10.81 %) were given AA/AS Hb genotype. All couples with SS fetuses opted for MTP. Follow up HPLC was performed in 24 cases, out of which 18 (75 %) were correlated and 6 (25 %) were mismatched. In present study sensitivity of ARMS-PCR was 75 %. ARMS-PCR is a simple technique to be established initially for providing rapid prenatal diagnosis to the couples with known sickle cell mutation. The sensitivity of ARMS-PCR can be increased by using suitable techniques to detect maternal cell DNA contamination.

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Section: Cvs and Fetal Genotypementioning

confidence: 99%

Prenatal Diagnosis of Sickle Cell Disease by the Technique of PCR

Singh

Shrivastava

Shrikhande

2014

Indian J Hematol Blood Transfus

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“…However, operators must be skilled in both methods. Both techniques appear to be comparably efficient between 8 and 12 weeks, when the overall success rate after two sampling device insertions is considered to be very near to 100% (Philip et al,2004).This efficiency has been confirmed in three national randomized trials of transabdominal vs.transcervical CVS (Brambati et al,1991;Jackson et al,1992;Smidt-Jensen et al,1992). Although the data appear to confirm that the two techniques are equally effective in obtaining adequate amounts of chorionic tissue, transabdominal needling entailed a significantly smaller proportion of repeated device insertions (3.3 vs. 10.3%) and of low weight specimens (3.2 vs. 4.9%).…”

Section: Transcervical Versus Transabdominal Chorionic Villus Samplingmentioning

confidence: 77%

46 Invasive Prenatal Diagnosis

Merz¹,

Bahlmann²,

Bernaschek³

et al. 2005

Gastroenterologie Compact

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“…We have not practiced transcervical CVS in a comparable number of patients but several randomized trials show almost identical miscarriage rates after transcervical CVS compared with the transabdominal approach. 5,6 It is important to consider is that there is no increased risks of limb reduction defects following CVS at 10 weeks or greater, but there is an increase in Talipes from early amniocentesis. Hence in the first trimester Chorionic villous Sampling appears to be a safer procedure.…”

Section: Discussionmentioning

confidence: 99%