A randomised clinical trial to determine the effect of a toothpaste containing enzymes and proteins on gum health over 3 months

Daly, Sinéad; Seong, Joon; Newcombe, Robert G.; Davies, Malcolm; Nicholson, James M.; Edwards, M I; West, Nicola

doi:10.1016/j.jdent.2018.12.002

Cited by 28 publications

(22 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lactoferrin, presented in the BM toothpaste, is a protein that possesses iron binding/transferring, antibacterial, antiviral, antifungal, anti-inflammatory, and anticarcinogenic properties [18]. A recent randomized clinical trial has demonstrated that a toothpaste containing enzymes and proteins was effective at preventing gingivitis compared to toothbrushing with a commercially available fluoride toothpaste [19].…”

Section: Discussionmentioning

confidence: 99%

Effects of Active Oxygen Toothpaste in Supragingival Biofilm Reduction: A Randomized Controlled Clinical Trial

Cunha

Auersvald

Deliberador

et al. 2019

International Journal of Dentistry

View full text Add to dashboard Cite

Gingivitis is still considered a major risk factor for the occurrence and progression of periodontal disease. The aim of the present study was to compare the long-term (1, 12, and 18 weeks) antiplaque and antigingivitis efficacies of two commercially available toothpastes, Colgate Total® (CT) and BlueM® (BM), against attached supragingival dental plaque and gingival inflammation in an experimental gingivitis model. A parallel double-blinded randomized clinical trial including 39 dental students who refrained from all plaque control methods (manual or chemical) for 7 days was conducted. After the establishment of clinical gingivitis, participants were randomized into two experimental groups (CT and BM). Plaque index (PI) and gingival index (GI) were then calculated according to Turesky’s modified Quigley and Hein index. Participants were assessed in four time periods (preclinical trial phase (W −1), gingivitis phase (W0), one week (W1), twelve weeks (W12), and eighteen weeks (W18)). Participants’ stimulated saliva was collected and cultured (either aerobically or anaerobically, 37°C, 48 hours) in each time period (W −1, W0, W1, W12, and W18) for the count of viable colonies. Obtained data were analyzed using 2-way ANOVA and Tukey’s test (α = 0.05). No significant differences were found (p>0.05) between experimental groups at W −1. Significant differences between groups were observed at W0 (p<0.05) for the parameter time period, but not for the interaction between parameters (time period ∗ toothpastes). Lower bacterial counts were observed in both groups after one week of toothbrushing; however, no significant differences were found between investigated dentifrices. Intra- and intergroup comparisons revealed that significant differences were not found (p>0.05) between dentifrices at W1, W12, and W18 for both GI and PI. The present study demonstrated that toothpastes containing active oxygen and lactoferrin (BM) have comparable antiplaque and antigingivitis efficacies with triclosan-containing toothpastes (CT).

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Active Oxygen Toothpaste in Supragingival Biofilm Reduction: A Randomized Controlled Clinical Trial

Cunha

Auersvald

Deliberador

et al. 2019

International Journal of Dentistry

View full text Add to dashboard Cite

show abstract

“…Commercially available dentifrices containing lactoperoxidase have been subjected to clinical trials involving participants from various age groups with various clinical conditions, e.g., malodor [159,160], xerostomia [161], caries [3], and chronic periodontitis [4], as well as healthy subjects [101,141,162,163,164]. The usefulness of LPO preparation was also determined during pilot studies in neonates under mechanical ventilation in order to avoid ventilator-associated pneumonia (VAP) [165].…”

Section: Clinical Applicationmentioning

confidence: 99%

“…Enzymatic toothpastes are frequently used in clinical trials in addition to tablets. Daly et al observed that the use of enzymatic paste in healthy subjects for 13 weeks significantly affected such parameters of gingival health, as modified gingival index, bleeding index, and plaque index, as compared to the control group using fluoride-only paste [164]. The same paste showed efficacy in increasing the permeability of the cell membrane (in the presence of 7.8 μg/mL paste proteins) and inhibiting the planktonic growth of F. nucleatum (in the presence of 1.4 mg/mL paste proteins).…”

Section: Clinical Applicationmentioning

confidence: 99%

The Significance of Lactoperoxidase System in Oral Health: Application and Efficacy in Oral Hygiene Products

Magacz

Kędziora

Sapa

et al. 2019

IJMS

View full text Add to dashboard Cite

Lactoperoxidase (LPO) present in saliva are an important element of the nonspecific immune response involved in maintaining oral health. The main role of this enzyme is to oxidize salivary thiocyanate ions (SCN−) in the presence of hydrogen peroxide (H2O2) to products that exhibit antimicrobial activity. LPO derived from bovine milk has found an application in food, cosmetics, and medical industries due to its structural and functional similarity to the human enzyme. Oral hygiene products enriched with the LPO system constitute an alternative to the classic fluoride caries prophylaxis. This review describes the physiological role of human salivary lactoperoxidase and compares the results of clinical trials and in vitro studies of LPO alone and complex dentifrices enriched with bovine LPO. The role of reactivators and inhibitors of LPO is discussed together with the possibility of using nanoparticles to increase the stabilization and activity of this enzyme.

show abstract

“…The worldwide prevalence of gingivitis is reported to be 46%, showing evidence of gingival bleeding and calculus across all the age groups. As per the global burden of disease study 2010, periodontitis is listed as sixth most prevalent human disease with standardized prevalence of 11.2% 5 . Poor oral hygiene due to accumulation of plaque is one of the most significant risk factor for gingivitis 6 .…”

Section: Introductionmentioning

confidence: 99%

“…The test toothpaste is fluoridated toothpaste that has a triple enzymatic system which includes lactoperoxidase, amyloglucosidase and glucose oxidase. Previous studies on enzymatic toothpaste have shown promising results 5,10 . However, the control toothpaste used in those studies was different than previously set standard choice of control toothpaste 11 .…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of efficacy of enzymatic toothpaste over standard fluoridated toothpaste in reduction of plaque and gingivitis

Subramanya

Prabhuji

Vardhan

et al. 2020

Int J Dental Hygiene

View full text Add to dashboard Cite

Objectives To compare and evaluate the efficacy of enzymatic toothpaste v/s standard fluoridated toothpaste in reduction of plaque and gingivitis during maintenance phase after non‐surgical periodontal therapy (NSPT). Materials and Methods A total of 52 participants belonging to age 20–50 years eligible for inclusion were randomly chosen from the outpatient department. Computer‐generated randomization was followed to recruit the subjects into two groups. 26 subjects were assigned to control group who were given with control toothpaste (standard fluoridated toothpaste), while 26 subjects were assigned to test group (enzymatic toothpaste) and given with test toothpaste. The amount of dental plaque and gingivitis were assessed baseline visit and at 6‐week follow‐up visit using Turesky‐Gilmore‐Glickman modification of the Quigley‐Hein plaque index (TQHPI) 1970, Gingival Index (GI) – (Löe and Sillness 1963) and Full Mouth Bleeding Score (FMBS), respectively. Data analysis was done using IBM SPSS® version 22. Results Intergroup comparison of TQHPI, GI and FMBS among test and control group has shown that test group had statistically significant lower values than the control group at the follow‐up visit. (p = 0.006*, p = 0.001*, p = 0.001*, respectively). Conclusions Within the limitations of our study, it can be concluded that test toothpaste has shown to be efficient than the previously set standard control fluoridated toothpaste in terms of reduction of plaque and gingivitis during maintenance phase after non‐surgical periodontal therapy (NSPT).

show abstract

A randomised clinical trial to determine the effect of a toothpaste containing enzymes and proteins on gum health over 3 months

Cited by 28 publications

References 50 publications

Effects of Active Oxygen Toothpaste in Supragingival Biofilm Reduction: A Randomized Controlled Clinical Trial

Effects of Active Oxygen Toothpaste in Supragingival Biofilm Reduction: A Randomized Controlled Clinical Trial

The Significance of Lactoperoxidase System in Oral Health: Application and Efficacy in Oral Hygiene Products

Comparative evaluation of efficacy of enzymatic toothpaste over standard fluoridated toothpaste in reduction of plaque and gingivitis

Contact Info

Product

Resources

About