A randomised, blinded, placebo-controlled clinical trial with allopurinol in canine leishmaniosis

Koutinas, A. F.; Saridomichelakis, M. N.; Mylonakis, Mathios E.; Leontides, Leonidas; Polizopoulou, Z. S.; Billinis, Charalambos; Argyriadis, Dimitris; Diakou, Αnastasia; Papadopoulos, O.

doi:10.1016/s0304-4017(01)00399-5

Cited by 101 publications

(86 citation statements)

References 35 publications

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“…The altered cutaneous homeostasis and the dysregulation of the immune system, including the depression of cell-mediated immunity, imposed by CVL may predispose to secondary infections, such as bacterial pyoderma (Koutinas et al, 2001). The similar reason may exist in the increased frequency of mycotic isolation from the skin of dogs with CVL.…”

Section: Resultsmentioning

confidence: 96%

Investigation of bacterial and fungal agents from cutaneous lesions in canine Leishmaniasis

Parın

Erbaş

Ural

et al. 2017

IJAR

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The objective of the present study was to determine the bacterial and fungal agents accompanying with cutaneous lesions of dogs suffering from Canine Visceral Leishmaniasis (CVL). Sterile swap samples were taken from cutaneous lesions inspected of ears, dorsal, collar, pectoral, inguinal and interdigital spaces of 20 dogs, in which diagnosis were based on a combination of clinical symptoms, anti-leishmania antibody titers by use of Immunofluorescence antibody test (IFAT), and dogs were further classified according to the Leishvet Guidelines involving serological, hematological, serum biochemical, and urinalysis. Swap samples were inoculated to culture media for isolation and identification of bacterial and mycotic agents. 48 Staphylococcus aureus, 4 Bacillus cereus, 8 Staphylococcus epidermidis, 24 Candida albicans, 24 Microsporium canis and 4 Aspergillus niger were isolated and identified in both group of dogs. Regarding antibiotic susceptibility test, the isolates were found 100% susceptible to cefoperazone and amoxicillin-clavulanic acid, 53% to danofloxacin, 46% of the isolates were susceptible to enrofloxacin, and all of the isolates were resistant (100%) to penicillin G and gentamicin.In conclusion, the antibacterial and antifungal therapy should be provided in accordance with antibiotic susceptibility tests in skin lesions of dogs suffering with CVL.

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Section: Resultsmentioning

confidence: 96%

Investigation of bacterial and fungal agents from cutaneous lesions in canine Leishmaniasis

Parın

Erbaş

Ural

et al. 2017

IJAR

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“…No presente estudo, a proporção de animais parasitologicamente positivos na punção de medula óssea foi de 50% para crista ilíaca e de 46,87% para tíbio-femural, similar ao encontrado por Koutinas et al (2001) e Ferrer et al (1999, que foi de 50 a 83% para amostras colhidas desse órgão, independente da região colhida. Para amostras de linfonodos pré-escapular e poplíteo, encontramos 37,5%, em comparação com 30 a 85% verificados pelos autores acima citados.…”

Section: Discussionunclassified

Leishmania spp. amastigotes in lymph nodes, bone marrow, spleen, skin and blood of naturally infected dogs

Furtado¹,

Viol²,

Babo-Terra³

2015

Pubvet

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Visceral leishmaniasis is an anthropozoonosis caused by the protozoa of the genus Leishmania and is transmitted by sandflies of the genus Lutzomyia. Dogs are considered the major reservoirs of parasite, being important for the maintence of the cycle of the disease. In recent years, the disease has been spreading throughout many regions of Brazil, with a growing and worrying prevalence in Mato Grosso do Sul, mainly in Campo Grande. The diagnosis of canine leishmaniasis should be done based on clinical signs, visualization of the parasite in biopsy samples from bone marrow and lymph nodes, serologic tests such as indirect immunofluorescence (IFA), ELISA, molecular methods such as Polymerase Chain Reaction (PCR) or even tissue culture. The purpose of this work was to observe the presence of amastigote forms of Leishmania spp. In samples obtained through biopsy of lymph nodes, bone marrow, spleen, peripheral blood and skin from ELISA and IFA positive dogs. Among the 32 animals studied, the presence of Leishmania forms was observed in all studied tissues of 32 dogs. It was also verified the presence of inclusions suggestive of Ehrlichia canis in 12 dogs, from witch 4 dos were negative for Leishmania spp. In cytological examinations, reinforcing the need for better confirming methods for canine leishmaniasis.

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“…8,15,21,22 This reduction is thought to be related to attenuated antigenic stimulation resulting from the decrease in parasite load. Our results might indicate that antigens such as rKMPII or rTRYP could be more sensitive than CTLA in detecting early reduction of Leishmania load in treated dogs, although the presence of seropositive dogs for these recombinant proteins at the end of the study could indicate persistence of subclinical infection.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have reported a significant decrease in levels of specific antibodies (mainly IgG) against crude Leishmania antigen in the follow-up of treated infected dogs. 15,16,21 This decrease in concentrations has been described in responsive and in nonresponsive dogs. 8,22 Therefore, seroreactivity against crude total parasite antigen cannot predict the outcome of treatment.…”

Section: Introductionmentioning

confidence: 98%

Dynamics and Predictive Potential of Antibodies against Insect-Derived Recombinant Leishmania infantum Proteins during Chemotherapy of Naturally Infected Dogs

Todolí

Galindo²,

Gómez-Sebastián³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

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A randomised, blinded, placebo-controlled clinical trial with allopurinol in canine leishmaniosis

Cited by 101 publications

References 35 publications

Investigation of bacterial and fungal agents from cutaneous lesions in canine Leishmaniasis

Investigation of bacterial and fungal agents from cutaneous lesions in canine Leishmaniasis

Leishmania spp. amastigotes in lymph nodes, bone marrow, spleen, skin and blood of naturally infected dogs

Dynamics and Predictive Potential of Antibodies against Insect-Derived Recombinant Leishmania infantum Proteins during Chemotherapy of Naturally Infected Dogs

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