A RALDH-like enzyme involved in Fusarium verticillioides development

Díaz-Sánchez, Violeta; Limón, M. Carmen; Schaub, Patrick; Al‐Babili, Salim; Ávalos, Javier

doi:10.1016/j.fgb.2015.12.005

Cited by 7 publications

(11 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Fusarium genomes contain a gene for an aldehyde dehydrogenase highly similar to RALDH enzymes converting retinal to retinoic acid in mammals. This enzyme, that was called CarY, was investigated in F. verticillioides and found to exhibit such enzymatic activity in vitro [ 41 ]. Targeted mutation of the carY gene in F. verticillioides had no effect on carotenogenesis, but resulted in diverse developmental alterations.…”

Section: Genes and Enzymes Of Carotenoid Metabolism In Fmentioning

confidence: 99%

Carotenoid Biosynthesis in Fusarium

Ávalos

Pardo-Medina

Parra-Rivero

et al. 2017

JoF

Self Cite

View full text Add to dashboard Cite

Many fungi of the genus Fusarium stand out for the complexity of their secondary metabolism. Individual species may differ in their metabolic capacities, but they usually share the ability to synthesize carotenoids, a family of hydrophobic terpenoid pigments widely distributed in nature. Early studies on carotenoid biosynthesis in Fusarium aquaeductuum have been recently extended in Fusarium fujikuroi and Fusarium oxysporum, well-known biotechnological and phytopathogenic models, respectively. The major Fusarium carotenoid is neurosporaxanthin, a carboxylic xanthophyll synthesized from geranylgeranyl pyrophosphate through the activity of four enzymes, encoded by the genes carRA, carB, carT and carD. These fungi produce also minor amounts of β-carotene, which may be cleaved by the CarX oxygenase to produce retinal, the rhodopsin’s chromophore. The genes needed to produce retinal are organized in a gene cluster with a rhodopsin gene, while other carotenoid genes are not linked. In the investigated Fusarium species, the synthesis of carotenoids is induced by light through the transcriptional induction of the structural genes. In some species, deep-pigmented mutants with up-regulated expression of these genes are affected in the regulatory gene carS. The molecular mechanisms underlying the control by light and by the CarS protein are currently under investigation.

show abstract

Section: Genes and Enzymes Of Carotenoid Metabolism In Fmentioning

confidence: 99%

Carotenoid Biosynthesis in Fusarium

Ávalos

Pardo-Medina

Parra-Rivero

et al. 2017

JoF

Self Cite

View full text Add to dashboard Cite

show abstract

“…Five different ADHs, ADHComp11367 (accession number KU577907) (Q9LLR2, W8SQV6 and M8AU02), ADHComp3898 (accession number KU577905) (A0A061E1Z5, A0A0B2PSM8, Q8RVW2 and W9RMTS), ADHComp54788 (accession number KU577904) (A0A059BY89 and I1QYB5), ADHComp20158 (accession number KU577906) (K9N4H5) and ADHComp2946 (accession number KU577903) (A0A072TS23 and Q9LRI6), were identified. Phylogenetic analyses revealed that one ADH ( ADHComp54788 ) was closely related to carD , involved in the conversion of β-apo-4′-carotenal in β-apo-4′-carotenol [ 30 ], and showed its highest expression levels at anthesis, while the four others ADHs were more closely related to retinal aldehyde dehydrogenases [ 31 , 32 ], being ADHComp11367 and ADHComp2946 with the highest expression level at anthesis stage, whereas ADHComp3898 and ADHComp20158 at orange and white stages, respectively ( Figure S2 ).…”

Section: Resultsmentioning

confidence: 99%

Unraveling Massive Crocins Transport and Accumulation through Proteome and Microscopy Tools during the Development of Saffron Stigma

Gómez-Gómez

Parra-Vega

Rivas-Sendra

et al. 2017

IJMS

View full text Add to dashboard Cite

Abstract:Crocins, the glucosides of crocetin, are present at high concentrations in saffron stigmas and accumulate in the vacuole. However, the biogenesis of the saffron chromoplast, the changes during the development of the stigma and the transport of crocins to the vacuole, are processes that remain poorly understood. We studied the process of chromoplast differentiation in saffron throughout stigma development by means of transmission electron microscopy. Our results provided an overview of a massive transport of crocins to the vacuole in the later developmental stages, when electron dense drops of a much greater size than plastoglobules (here defined "crocinoplast") were observed in the chromoplast, connected to the vacuole with a subsequent transfer of these large globules inside the vacuole. A proteome analysis of chromoplasts from saffron stigma allowed the identification of several well-known plastid proteins and new candidates involved in crocetin metabolism. Furthermore, expressions throughout five developmental stages of candidate genes responsible for carotenoid and apocarotenoid biogenesis, crocins transport to the vacuole and starch metabolism were analyzed. Correlation matrices and networks were exploited to identify a series of transcripts highly associated to crocetin (such as 1-Deoxy-D-xylulose 5-phosphate synthase (DXS), 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), carotenoid isomerase (CRTISO), Crocetin glucosyltransferase 2 (UGT2), etc.) and crocin (e.g., ζ-carotene desaturase (ZDS) and plastid-lipid-associated proteins (PLAP2)) accumulation; in addition, candidate aldehyde dehydrogenase (ADH) genes were highlighted.

show abstract

“…Carotenoids were extracted and analyzed as previously described [ 18 , 19 ]. Briefly, carotenoids were extracted with acetone from freeze dried mycelia and were separated by thin layer chromatography developed in light petroleum/diethyl ether/acetone (4:1:1; v/v/v).…”

Section: Methodsmentioning

confidence: 99%

CoIN: co-inducible nitrate expression system for secondary metabolites in Aspergillus nidulans

Wiemann

Soukup

Folz

et al. 2018

Fungal Biol Biotechnol

View full text Add to dashboard Cite

BackgroundSequencing of fungal species has demonstrated the existence of thousands of putative secondary metabolite gene clusters, the majority of them harboring a unique set of genes thought to participate in production of distinct small molecules. Despite the ready identification of key enzymes and potential cluster genes by bioinformatics techniques in sequenced genomes, the expression and identification of fungal secondary metabolites in the native host is often hampered as the genes might not be expressed under laboratory conditions and the species might not be amenable to genetic manipulation. To overcome these restrictions, we developed an inducible expression system in the genetic model Aspergillus nidulans.ResultsWe genetically engineered a strain of A. nidulans devoid of producing eight of the most abundant endogenous secondary metabolites to express the sterigmatocystin Zn(II)2Cys6 transcription factor-encoding gene aflR and its cofactor aflS under control of the nitrate inducible niiA/niaD promoter. Furthermore, we identified a subset of promoters from the sterigmatocystin gene cluster that are under nitrate-inducible AflR/S control in our production strain in order to yield coordinated expression without the risks from reusing a single inducible promoter. As proof of concept, we used this system to produce β-carotene from the carotenoid gene cluster of Fusarium fujikuroi.ConclusionUtilizing one-step yeast recombinational cloning, we developed an inducible expression system in the genetic model A. nidulans and show that it can be successfully used to produce commercially valuable metabolites.Electronic supplementary materialThe online version of this article (10.1186/s40694-018-0049-2) contains supplementary material, which is available to authorized users.

show abstract

A RALDH-like enzyme involved in Fusarium verticillioides development

Cited by 7 publications

References 54 publications

Carotenoid Biosynthesis in Fusarium

Carotenoid Biosynthesis in Fusarium

Unraveling Massive Crocins Transport and Accumulation through Proteome and Microscopy Tools during the Development of Saffron Stigma

CoIN: co-inducible nitrate expression system for secondary metabolites in Aspergillus nidulans

Contact Info

Product

Resources

About