A rainbow trout SRY‐type gene expressed in pituitary glands

Ito, Michihiko; Ishikawa, Masako; Suzuki, Shin; Takamatsu, Nobuhiko; Shiba, Tadayoshi

doi:10.1016/0014-5793(95)01307-5

Cited by 31 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recombinant SOX13 was tested for binding to radiolabeled double-stranded DNA bearing the high-affinity SOX binding motif AACAAT (29,34,35). SOX13(66-604) protein bound the AACAAT sequence but not the CCGCGG sequence ( F i g .…”

Section: A C B Dmentioning

confidence: 99%

Sex-determining region Y-related protein SOX13 is a diabetes autoantigen expressed in pancreatic islets.

et al. 2000

View full text Add to dashboard Cite

The SOX (sex-determining region [SRY]-type high mobility group [HMG] box) family of transcription factors play key roles in determining cell fate during organ development. In this study, we have identified a new human SOX gene, S O X 1 3, as encoding the type 1 diabetes autoantigen, islet cell antigen 12 (ICA12). Sequence analysis showed that SOX13 belongs to the class D subgroup of SOX transcription factors, which contain a leucine zipper motif and a region rich in glutamine. SOX13 autoantibodies occurred at a significantly higher frequency among 188 people with type 1 diabetes (18%) than among 88 with type 2 diabetes (6%) or 175 healthy control subjects (4%). Deletion mapping of the antibody epitopes showed that the autoantibodies were primarily directed against an epitope requiring the majority of the protein. S O X 1 3 R N A was detected in most human tissues, with the highest levels in the pancreas, placenta, and kidney. Immunohistochemistry on sections of human pancreas identified SOX13 in the islets of Langerhans, where staining was mostly cytoplasmic. In mouse pancreas, Sox13 was present in the nucleus and cytoplasm of -cells as well as other islet cell types. Recombinant SOX13 protein bound to the SOX consensus DNA motif AACAAT, and binding was inhibited by homodimer formation. These observations-along with the known molecular interactions of the closely related protein, rainbow trout Sox23-suggest that SOX13 may be activated for nuclear import and DNA binding through heterodimer formation. In conclusion, we have identified ICA12 as the putative transcription factor SOX13 and demonstrated an increased frequency of autoantibody reactivity in sera from type 1 diabetic subjects compared with type 2 diabetic and healthy control subjects. T ype 1 diabetes results from the destruction of pancreatic islet -cells by an autoimmune response to -cell constituents, which is initiated by an unknown environmental agent acting on a susceptible genetic background. Identification of GAD (1), a family of tyrosine phosphatase-like proteins variously designated islet cell antigen 512 (ICA512)/IA-2/IA-2 (2,3), and insulin (4) as autoantigens in type 1 diabetes has led to the proposal of several mechanisms for the initiation and progression of islet autoimmunity. These include molecular mimicry between GAD and coxsackievirus B (5) or between ICA512 and rotavirus (6), hyperexpression of GAD in response to metabolic stress (7), and lack of tolerance to (pro)insulin as a consequence of genetic variation of proinsulin expression levels in the thymus (8,9). The multifactorial nature of type 1 diabetes and the association of numerous environmental agents with the disease (10) suggest that there may be multiple pathways leading to -cell autoimm u n i t y. To attain a better understanding of the pathogenesis of type 1 diabetes, the autoimmune response to islet -c e l l s needs to be fully characterized.In a search for novel diabetes-associated autoantigens, the screen of an islet cDNA expression library with type 1 diabetes sera tha...

show abstract

Section: A C B Dmentioning

confidence: 99%

Sex-determining region Y-related protein SOX13 is a diabetes autoantigen expressed in pancreatic islets.

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Sox10 belongs to the same subgroup of Sox proteins as Sox8 and Sox9, as indicated by the fact that the HMG domains of all three proteins share Ͼ90% identity (Wright et al, 1993). Sequence similarities were apparent even outside the (Wright et al, 1995), mouse Sox8 (Wright et al, 1993), and rainbow trout SoxP1 (Ito et al, 1995). E xact matches between Sox10 and the aligned sequences are marked by asterisks.…”

Section: Identification Of Sox10mentioning

confidence: 99%

“…1C). When amino acid sequences of rat Sox10 and mouse Sox9 (Wright et al, 1995) were aligned with sequences of SoxP1, the putative Sox8 ortholog from rainbow trout (Ito et al, 1995), we detected three additional regions with significant sequence conservation. One of them corresponded to amino acids 233-306 of Sox10 with 61% identity over a stretch of 74 amino acids, and a second one corresponded to the C -terminal region of Sox10 with 41% identity over a stretch of 66 amino acids.…”

Section: Identification Of Sox10mentioning

confidence: 99%

Sox10, a Novel Transcriptional Modulator in Glial Cells

et al. 1998

View full text Add to dashboard Cite

Sox proteins are characterized by possession of a DNA-binding domain with similarity to the high-mobility group domain of the sex determining factor SRY. Here, we report on Sox10, a novel protein with predominant expression in glial cells of the nervous system. During development Sox10 first appeared in the forming neural crest and continued to be expressed as these cells contributed to the forming PNS and finally differentiated into Schwann cells. In the CNS, Sox10 transcripts were originally confined to glial precursors and later detected in oligodendrocytes of the adult brain. Functional studies failed to reveal autonomous transcriptional activity for Sox10. Instead, Sox10 functioned synergistically with the POU domain protein Tst-1/Oct6/SCIP with which it is coexpressed during certain stages of Schwann cell development. Synergy depended on binding to adjacent sites in target promoters, was mediated by the N-terminal regions of both proteins, and could not be observed between Sox10 and several other POU domain proteins. Interestingly, Sox10 also modulated the function of Pax3 and Krox-20, two other transcription factors involved in Schwann cell development. We propose a role for Sox10 in conferring cell specificity to the function of other transcription factors in developing and mature glia.

show abstract

“…The SRY gene belongs to a rapidly growing family of genes that are related by sequence homology to the HMG box, named Sox genes (SRY-like HMG-box gene). The members of the Sox gene family have been conserved through evolution, and have been found in a wide variety of species including humans [7], mice [36], marsupials [11], birds [14], turtles [26], Xenopus [23], alligators, lizards, Drosophila [6] and fishes [12,15,29,30,32]. Although recent studies show that some Sox genes have important developmental roles, many of them have not been identified.…”

Section: Introductionmentioning

confidence: 99%

SRY-related genes in the genome of the rice field eel (Monopterus albus)

et al. 2002

View full text Add to dashboard Cite

-The mammalian sex determining gene, SRY, is the founding member of the new growing family of Sox (SRY-like HMG-box gene) genes. Sox genes encode transcription factors with diverse roles in development, and a few of them are involved in sex determination and differentiation. We report here the existence of Sox genes in the rice field eel, Monopterus albus, and DNA sequence information of the HMG box region of five Sox genes. The Sox1, Sox4 and Sox14 genes do not have introns in the HMG box region. The Sox9 gene and Sox17 gene, which each have an intron in the conserved region, show strong identity at the amino acid level with the corresponding genes of mammals and chickens. Similar structure and identity of the Sox9 and Sox17 genes among mammals, chickens and fish suggest that these genes have evolutionarily conserved roles, potentially including sex determination and differentiation. fish / Sox / cloning / sex determination

show abstract

A rainbow trout SRY‐type gene expressed in pituitary glands

Cited by 31 publications

References 20 publications

Sex-determining region Y-related protein SOX13 is a diabetes autoantigen expressed in pancreatic islets.

Sex-determining region Y-related protein SOX13 is a diabetes autoantigen expressed in pancreatic islets.

Sox10, a Novel Transcriptional Modulator in Glial Cells

SRY-related genes in the genome of the rice field eel (Monopterus albus)

Contact Info

Product

Resources

About