A raft technique for chemotaxis: A versatile method suitable for clinical studies

Addison, I E; Babbage, J

doi:10.1016/0022-1759(76)90033-8

Cited by 40 publications

(9 citation statements)

References 3 publications

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“…Because wNe wished to combine measurement of phagocytosis and killing in the same test we chose a 2:1 ratio. Addison & Babbage (1976). It is a membrane-migration technique allowing the simultaneous comparison of several samples.…”

Section: Methodsmentioning

confidence: 99%

Neutrophil function during chemotherapy for Hodgkin's disease

Gandossini¹,

Souhami²,

Babbage³

et al. 1981

Br J Cancer

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Summary.-Simultaneous measurement of neutrophil migration, phagocytic activity, candidacidal and bactericidal activity were made during quadruple chemotherapy of advanced Hodgkin's disease (HD). Measurements were also made in normal individuals, hospital patients not on chemotherapy, untreated patients with advanced HD and patients off chemotherapy for over a year.Neutrophil migratory activity was usually normal in untreated HD patients and those on chemotherapy, but > 200O of all tests showed depressed values, some of which were corrected by plasma. Similar results were found with neutrophil phagocytosis. Abnormalities in these functions were found in both early and late cycles, but there was a tendency for migration to deteriorate during later chemotherapy cycles. Neutrophil candidacidal and bactericidal activity were frequently depressed in patients on treatment and there was deterioration in candidacidal activity during the chemotherapy cycle. These abnormalities of killing activity were frequently corrected in control plasma.Neutrophil function is normal in most patients with advanced HD and in patients in remission. In a minority of patients on treatment there are marked functional defects, especially in killing activity. These defects are partly cell-associated and partly plasma-related. Susceptibility to infection during chemotherapy of HD may be partly due to defective neutrophil function.

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Section: Methodsmentioning

confidence: 99%

Neutrophil function during chemotherapy for Hodgkin's disease

Gandossini¹,

Souhami²,

Babbage³

et al. 1981

Br J Cancer

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“…Polymorphonuclear and monocyte migration, assessed by a raft technique (Addison & Babbage, 1976) was found to be normal. The neutrophil phagocytosis index (Bridges, Dasilva& Valdimarsson, 1980) was reduced at 2'i (normal 3'i-4-9), but intrinsic ability of neutrophils to kill Candida albicans was normal.…”

Section: Investigationsmentioning

confidence: 96%

Idiopathic pyoderma gangrenosum and impaired lymphocyte function: failure of azathioprine and corticosteroid therapy

1981

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A 58-year-old man with chronic "idiopathic' pyoderma gangrenosum, recurrent secondary staphylococcal infection, nail candidiasis, markedly impaired lymphocyte function and a serum blocking factor is described. Despite an initially favourable response to immunosuppressive therapy, the patient later relapsed whilst on high dose systemic corticosteroids and azathioprine. Immune function remains abnormal 3 years cessation of azathioprine. Attempted immunostimulation with Corynebacterium parvum proved unsuccessful. A striking but transient increase in lymphocyte function followed parenteral iron therapy for an unexplained iron-deficiency anaemia. Gradual reduction in prednisone dosage to 5 mg daily has coincided with a period of clinical resolution and modest improvement in lymphocyte reactivity. Prolonged immunosuppressive therapy may be inadvisable in chronic idiopathic pyoderma gangrenosum associated with defective cellular immunity.

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“…Migration was measured using Addison's "raft" adaptation (Addison and Babbage, 1976) of Boyden's chamber technique (Boyden, 1962). Aliquots of the above cell suspensions were sedimented on the bench centrifuge and resuspended in the same media at concentrations of 2x1O6/ml, exce t for the W-256 cells which were resuspended at 10 /ml.…”

Section: Measurement Of Migrationmentioning

confidence: 99%

Some effects of products from necrotic regions of tumours on the in vitro migration of cancer and peritoneal exudate cells

Turner

Weiss

1980

Intl Journal of Cancer

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The invasion of normal tissues by cancer cells and the infiltration of tumours by macrophages to some degree involves active translatory movements by these cells. As necrosis is a common occurrence in solid tumours, we have studied the interactions of saline extracts from the necrotic regions of rat Walker-256 tumours and mouse Gardner lymphosarcomas on the transmembrane, in vitro migration of Walker and Gardner cancer cells, and rat and mouse peritoneal macrophages. Necrotic extracts enhanced cell migration independently of chemotactic activity, an effect which was partially reduced by Trasylol, an inhibitor of neutral proteases. Rat liver lysosomal preparations which contain lower levels of neutral proteases than necrotic extract, inhibited or had no effects on cell migration, and the lysosomal stabilizer hydrocortisone did not inhibit the action of necrotic extracts. The results indicate that necrosis may enhance the migration of cancer cells out of tumours and the migration of macrophages into them. The extracts act partially through their neutral protease content. In contrast to the enhancement of cell detachment by necrotic extracts, which previous work has shown to be partially mediated by their indirect effect in causing lysosomal labilization and which is partially inhibited by hydrocortisone, this indirect mechanism is not demonstrable in the enhancement of cell migration. The results indicate that the consequences of necrosis are worthy of consideration in attempts at understanding the biology of solid tumours.

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A raft technique for chemotaxis: A versatile method suitable for clinical studies

Cited by 40 publications

References 3 publications

Neutrophil function during chemotherapy for Hodgkin's disease

Neutrophil function during chemotherapy for Hodgkin's disease

Idiopathic pyoderma gangrenosum and impaired lymphocyte function: failure of azathioprine and corticosteroid therapy

Some effects of products from necrotic regions of tumours on the in vitro migration of cancer and peritoneal exudate cells

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